##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926792_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 789766 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.701210231891473 31.0 30.0 31.0 23.0 34.0 2 28.761509105228637 31.0 30.0 31.0 23.0 34.0 3 28.331957820417692 31.0 28.0 31.0 16.0 34.0 4 32.2014267517214 35.0 32.0 37.0 23.0 37.0 5 32.06409620064677 35.0 32.0 37.0 22.0 37.0 6 31.686536265172215 35.0 32.0 37.0 17.0 37.0 7 31.548938546354236 35.0 32.0 37.0 17.0 37.0 8 31.683247949392605 35.0 32.0 37.0 17.0 37.0 9 33.873316906526746 37.0 34.0 39.0 24.0 39.0 10 34.02113410807758 38.0 34.0 39.0 25.0 39.0 11 34.18102577219075 38.0 35.0 39.0 26.0 39.0 12 34.06746681928571 38.0 35.0 39.0 25.0 39.0 13 34.175964779440996 38.0 35.0 39.0 26.0 39.0 14 35.23986978421456 39.0 36.0 41.0 26.0 41.0 15 34.85189283914476 39.0 35.0 41.0 22.0 41.0 16 34.93405768290861 39.0 35.0 41.0 24.0 41.0 17 35.160561482768315 39.0 36.0 41.0 25.0 41.0 18 35.20688026580025 40.0 36.0 41.0 25.0 41.0 19 34.612307949443256 39.0 34.0 41.0 18.0 41.0 20 34.90624817984061 39.0 35.0 41.0 23.0 41.0 21 34.89040677871673 39.0 35.0 41.0 22.0 41.0 22 34.9123816421573 39.0 35.0 41.0 22.0 41.0 23 34.91601689614392 39.0 36.0 41.0 23.0 41.0 24 33.64811729043793 39.0 33.0 41.0 10.0 41.0 25 34.235007584525036 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.043814635072941144 1101 2 -0.09937545975899553 1101 3 -1.2682244846042607 1101 4 -0.39071458036267614 1101 5 -0.3038482823799349 1101 6 -2.0306621905702613 1101 7 -0.5327158050817857 1101 8 0.0756294366499688 1101 9 0.014866282045737478 1101 10 -9.184933874828218 1101 11 -3.6719524416398848 1101 12 -1.264697140253979 1101 13 -0.2971762492154255 1101 14 -0.10377383960658904 1101 15 -1.6573528744704689 1101 16 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2214 23 -0.5504131845340225 2214 24 -1.6129967354031152 2214 25 -0.5628147077062309 2215 1 0.665616121086785 2215 2 0.2709806072835619 2215 3 0.9052519964338153 2215 4 0.9362407690689238 2215 5 1.224227719782725 2215 6 1.6034194148560523 2215 7 1.0026135446447242 2215 8 -0.08715309633415558 2215 9 0.43290626322775694 2215 10 0.09758857684538924 2215 11 0.14265536501240916 2215 12 0.03799846490552028 2215 13 0.46417948781034823 2215 14 0.35122099949267493 2215 15 0.3128894421069077 2215 16 0.48971394530308743 2215 17 0.11478626794853142 2215 18 -0.6487077817999491 2215 19 0.6181144111941634 2215 20 -3.1076789371814293 2215 21 -1.2411917799114462 2215 22 -0.49491136654750534 2215 23 -0.3658318464662287 2215 24 -0.4905938010698492 2215 25 -0.25560708680535527 2216 1 -0.9796467485736748 2216 2 -1.4028904969681548 2216 3 -0.44819692321170734 2216 4 -0.9422136949177506 2216 5 -0.7616393020235073 2216 6 -2.1678357787206544 2216 7 -9.705522153670703 2216 8 -11.174679573391899 2216 9 -6.212533120150987 2216 10 -3.907426223150992 2216 11 -2.9259292665265164 2216 12 -2.489676283675582 2216 13 -2.446282231342746 2216 14 -2.320688652639518 2216 15 -3.5165920827905772 2216 16 -2.4686610648803935 2216 17 -2.676045541633904 2216 18 -2.3824458897539316 2216 19 -1.8083597046531423 2216 20 -2.632260735448881 2216 21 -5.0905661745599105 2216 22 -3.7183466176948414 2216 23 -3.4639785655740667 2216 24 -3.483519674360153 2216 25 -3.2202477682388313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36336.0 3 6.0 4 284.0 5 1692.0 6 2134.0 7 1728.0 8 1390.0 9 1246.0 10 1197.0 11 1230.0 12 1135.0 13 1182.0 14 1186.0 15 1308.0 16 1331.0 17 1476.0 18 1663.0 19 2187.0 20 2808.0 21 3582.0 22 4523.0 23 5759.0 24 6617.0 25 7636.0 26 8863.0 27 9677.0 28 10878.0 29 12718.0 30 15351.0 31 19021.0 32 24695.0 33 32848.0 34 45177.0 35 63851.0 36 93575.0 37 152706.0 38 210770.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29778619423918 18.74881008892285 12.18126248613357 25.772141230704403 2 13.667527639248684 20.296636120526017 43.570284653512424 22.465551586712873 3 19.81040202075535 24.2249713421995 29.07016849772259 26.89445813932256 4 10.996006244044844 17.178979058122327 39.843797514545784 31.981217183287043 5 11.867530076002144 38.57061258917682 36.92987311023667 12.63198422458437 6 30.6943721496319 39.06896101905923 18.50983610502146 11.726830726287407 7 27.477451162176376 31.16121117294597 22.501847849002164 18.8594898158755 8 23.226612620575104 39.48831456450736 21.599134449811803 15.68593836510573 9 25.773609756715338 15.61454388875521 20.461367003963982 38.15047935056547 10 13.129814890345266 30.617192626013644 36.23432191904018 20.018670564600914 11 34.43080519684461 22.52578096406812 25.132561582406005 17.910852256681267 12 22.612257097120626 25.183000344471235 33.47652529837288 18.728217260035258 13 29.072035175433932 22.662421705025825 25.839051114302432 22.426492005237815 14 19.10212962118679 23.500172233873336 28.573852871746553 28.823845273193317 15 22.075236340380805 33.36094067587334 23.18256938244401 21.38125360130185 16 20.299871074941173 29.327158101635497 30.08183828390362 20.29113253951971 17 20.424665878935016 29.866934140821595 29.132013439563124 20.57638654068026 18 19.892635162174347 28.920627523288978 31.531044369361382 19.655692945175293 19 22.896484293369436 27.58953912947664 27.811811730673995 21.702164846479924 20 22.275370082609253 28.81319012538927 29.380044350390882 19.531395441610595 21 21.826390339962895 27.849350024378044 27.62367899499142 22.700580640667642 22 21.25874462405082 30.113927064389813 28.399575496309655 20.227752815249715 23 20.942172330558165 29.37950057115937 28.573165387995775 21.105161710286694 24 22.223938908483035 28.001165095265534 29.65420732363687 20.120688672614563 25 21.50110872888128 28.727665539997187 29.256765399047417 20.514460332074115 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 194.0 1 194.0 2 267.5 3 341.0 4 341.0 5 341.0 6 533.0 7 725.0 8 725.0 9 725.0 10 1118.5 11 1512.0 12 1512.0 13 1512.0 14 2717.5 15 3923.0 16 3923.0 17 3923.0 18 6879.5 19 9836.0 20 9836.0 21 9836.0 22 15665.5 23 21495.0 24 21495.0 25 21495.0 26 31900.5 27 42306.0 28 42306.0 29 42306.0 30 56230.0 31 70154.0 32 70154.0 33 70154.0 34 79655.5 35 89157.0 36 89157.0 37 89157.0 38 94675.5 39 100194.0 40 100194.0 41 100194.0 42 103403.5 43 106613.0 44 106613.0 45 106613.0 46 106543.5 47 106474.0 48 106474.0 49 106474.0 50 99195.5 51 91917.0 52 91917.0 53 91917.0 54 77595.5 55 63274.0 56 63274.0 57 63274.0 58 52059.5 59 40845.0 60 40845.0 61 40845.0 62 31810.5 63 22776.0 64 22776.0 65 22776.0 66 16999.0 67 11222.0 68 11222.0 69 11222.0 70 7840.5 71 4459.0 72 4459.0 73 4459.0 74 3021.0 75 1583.0 76 1583.0 77 1583.0 78 1063.0 79 543.0 80 543.0 81 543.0 82 349.5 83 156.0 84 156.0 85 156.0 86 104.5 87 53.0 88 53.0 89 53.0 90 30.0 91 7.0 92 7.0 93 7.0 94 6.0 95 5.0 96 5.0 97 5.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23956463053613347 2 0.1421940169619862 3 0.14561275111868577 4 0.0676149644325028 5 0.02317142039540826 6 0.022158462126756533 7 0.2993291683865854 8 0.2899593044015569 9 0.01975268623870868 10 0.03646649767146218 11 0.016840431216334963 12 0.018992967537219886 13 0.01570085316410177 14 0.01798000926856816 15 0.015320993813357374 16 0.020259165373034543 17 0.019879306022290145 18 0.015447613596938841 19 0.024690857798385854 20 0.018359868619312554 21 0.015320993813357374 22 0.017473530134242295 23 0.01823324883573109 24 0.016587191649172033 25 0.015574233380520308 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 789766.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.85360305743033 #Duplication Level Percentage of deduplicated Percentage of total 1 94.8128647806785 81.40026057618188 2 3.9758584900577687 6.82683553235868 3 0.541484087360408 1.394650796944662 4 0.18493260973503953 0.6350852347426704 5 0.117849105456743 0.505888516027823 6 0.07399248241686908 0.38115127287910633 7 0.052465139034549066 0.3153024854717549 8 0.032454589609998676 0.22290747630149027 9 0.022404521229306387 0.17311579850813777 >10 0.15320853695753958 2.5469559466460683 >50 0.016538967152030078 1.0036929109923436 >100 0.01358421772205816 2.4722049930923458 >500 0.0014765453682246126 0.8361534166432057 >1k 8.859272209347674E-4 1.2857950432098295 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2394 0.30312776189402935 No Hit CTGTAGGACGTGGAATATGGCAAGA 1919 0.24298336469283305 No Hit GTCCTACAGTGTGCATTTCTCATTT 1727 0.2186723662451916 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1632 0.20664348680495237 No Hit CTTTAGGACGTGAAATATGGCGAGG 1302 0.1648589582230686 No Hit CTGTAGGACCTGGAATATGGCGAGA 1169 0.14801852700673362 No Hit GTATCAACGCAGAGTACTTTTTTTT 868 0.10990597214871241 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.266197835814659E-4 0.0 8 0.0 0.0 0.0 1.266197835814659E-4 0.0 9 0.0 0.0 0.0 1.266197835814659E-4 0.0 10 0.0 0.0 0.0 1.266197835814659E-4 0.0 11 0.0 0.0 0.0 1.266197835814659E-4 0.0 12 0.0 0.0 0.0 1.266197835814659E-4 0.0 13 0.0 0.0 0.0 1.266197835814659E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 375 0.0 14.217281 4 ATAGGAC 105 1.8981154E-8 12.708543 3 GCCGGTT 75 1.50461365E-5 12.643367 11 TGTCCAC 235 0.0 12.510447 10 GGCGAGG 380 0.0 12.477007 19 TCTATAC 115 5.0513336E-9 12.43227 3 TAAGGGT 85 3.8425933E-6 12.320648 4 CGGTTTC 70 1.10847715E-4 12.191818 13 ATTTAGA 265 0.0 11.880641 1 GGACCTG 650 0.0 11.861747 6 AGGACCT 720 0.0 11.501745 5 CCTACAC 150 1.6552804E-10 11.437689 3 GTCCTAC 1150 0.0 11.365657 1 TAATACT 145 1.1696102E-9 11.161966 4 CCGGTTT 85 5.415549E-5 11.155911 12 TCTAGAC 60 0.005728784 11.119975 3 TGTAGAA 515 0.0 11.104553 2 GTTATAT 105 3.2865446E-6 10.903446 1 GCCTATA 70 0.0014380031 10.903445 1 TCCTAGG 105 3.3243905E-6 10.893037 2 >>END_MODULE