FastQCFastQC Report
Thu 26 May 2016
SRR1926792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926792_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789766
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT23630.29920254860300394No Hit
CTGTAGGACGTGGAATATGGCAAGA22590.28603409111053146No Hit
GTCCTACAGTGTGCATTTCTCATTT17940.22715589174514983No Hit
GTCCTAAAGTGTGTATTTCTCATTT16780.21246799684969978No Hit
GTATCAACGCAGAGTACTTTTTTTT15550.19689376346917947No Hit
CTTTAGGACGTGAAATATGGCGAGG14970.18954981602145446No Hit
CTGTAGGACCTGGAATATGGCGAGA14290.18093967073791475No Hit
GGTATCAACGCAGAGTACTTTTTTT9710.12294780985760338No Hit
CTGAAGGACCTGGAATATGGCGAGA9120.1154772426262969No Hit
CACACACACACACACACACACACAC8760.11091893041736414No Hit
GATATACACTGTTCTACAAATCCCG7900.10002962902935805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGT250.006071220618.9773358
TCCAACG1200.015.02562518
CCAACGT456.8120006E-414.76201819
TAGGACC4800.014.4774274
CCAACGA903.680907E-813.70758819
CCGGTTT655.5071934E-513.13815412
GGCGAGG3500.013.01467819
TCGTCAA603.979673E-412.7070672
ATAAGGT604.0139712E-412.6941523
GTAGGAC12000.012.5354743
GGACCTG8350.012.3125226
TGTAGGA12700.012.3068452
CTGTAGG12500.012.2758071
AGGACCT8650.012.21565155
GTGTTAG1251.3351382E-912.199561
GTCCTAC12650.012.1302441
CGGTTTC550.003093680412.07648613
TCTGTAC1353.5652192E-1011.9889213
CTAGGAC1209.664291E-911.9007673
GTCGTCA657.800218E-411.7303461
Produced by FastQC (version 0.11.5)