FastQCFastQC Report
Thu 26 May 2016
SRR1926791_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926791_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870436
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19590.22505962529123336No Hit
CTGTAGGACGTGGAATATGGCAAGA15210.17474001534862987No Hit
GTCCTAAAGTGTGTATTTCTCATTT14620.16796180305042532No Hit
GTCCTACAGTGTGCATTTCTCATTT14460.1661236437831156No Hit
CTTTAGGACGTGAAATATGGCGAGG11240.12913068852850756No Hit
CTGTAGGACCTGGAATATGGCGAGA9250.10626858264134295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG307.6303823E-419.0326375
AGGCCGT400.00521886814.2736566
GTATTAG551.8881809E-413.877931
TAGGACC3500.012.5122134
TTTATAC1155.0567905E-912.4319463
ACGAAAT550.00310674212.0699212
GGCGAGG3100.011.93154919
CCCTATA657.7998865E-411.7306562
TAGGACT1552.3646862E-1111.6698754
GTATAAC907.1003797E-611.6613161
GTCCAAG907.1003797E-611.6613161
TGTAGGA10050.011.5701622
GTAGGAC9650.011.5559113
GTACTAA751.9904008E-411.4492921
GTATAAT1001.8287064E-611.4492921
CCTAATA752.0097307E-411.4373882
GTCCTAC10100.011.3359321
GACGTGG4300.011.2861467
CTGTAGG10150.011.186091
TACTAAC600.005730698411.1196842
Produced by FastQC (version 0.11.5)