FastQCFastQC Report
Thu 26 May 2016
SRR1926791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926791_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870436
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19600.22517451024544022No Hit
CTGTAGGACGTGGAATATGGCAAGA17380.1996700504115179No Hit
GTATCAACGCAGAGTACTTTTTTTT14030.16118359075222072No Hit
GTCCTAAAGTGTGTATTTCTCATTT13800.15854123680546303No Hit
CTTTAGGACGTGAAATATGGCGAGG13720.15762215717180816No Hit
GTCCTACAGTGTGCATTTCTCATTT13400.15394583863718872No Hit
CTGTAGGACCTGGAATATGGCGAGA10670.12258224613871668No Hit
GGTATCAACGCAGAGTACTTTTTTT8950.10282203401513725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3800.014.7875224
GTAGGAC9700.013.9450063
GGCGTGT551.9796171E-413.8007978
GACGTGG4800.013.63985357
TGTAGGA10700.013.5437182
AGGCGTG852.7340866E-713.3956627
CGTCAAG500.001468279513.3361413
AAGGGTA500.00147019613.3338345
TCCAACG655.5147935E-513.13654318
CTGTAGG10900.012.9453351
CGTGGAA5250.012.8314579
AAAGGCG905.232032E-712.6988895
GGACGTG8750.012.2997826
AGGACGT8950.012.2377575
TCGTCAA550.002985441612.1342642
GTCGTCA550.002985441612.1342641
ACGTGGA5400.012.1236188
GGACCTG6300.012.0941796
TGCCGGT550.003099625712.07361610
TAGGACG9300.011.7771954
Produced by FastQC (version 0.11.5)