FastQCFastQC Report
Thu 26 May 2016
SRR1926790_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926790_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866641
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18240.21046777154554194No Hit
CTGTAGGACGTGGAATATGGCAAGA14780.1705435122501705No Hit
GTCCTAAAGTGTGTATTTCTCATTT14230.16419717045466348No Hit
GTCCTACAGTGTGCATTTCTCATTT10830.12496523935516551No Hit
CTTTAGGACGTGAAATATGGCGAGG10820.12484985132251994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG852.5612462E-713.4676681
TAGGACC3550.012.8739454
TCTTATA751.4276702E-512.7083962
CTTATAC701.0568598E-412.2545253
GTATAAG802.7435655E-511.9244991
TCTACAC1459.094947E-1111.8319553
TCATAAC657.798866E-411.7308283
TGTAGGA7750.011.1915872
GTATTAG600.00569301711.1295311
GTCCTAC9200.011.0949671
CTACACT1303.1506715E-810.9861794
CTGTAGG7950.010.919541
CCTAGAC700.001449898910.8929123
GCCTTAG803.624936E-410.7320491
TCTATAC909.191208E-510.590333
TTGGACA909.2890186E-510.5792834
TAGCCCT2001.8189894E-1210.4734914
GTAGGAC7750.010.4536813
TCCTACA10700.010.3329942
CTTAGAC1201.4574434E-610.3255723
Produced by FastQC (version 0.11.5)