FastQCFastQC Report
Thu 26 May 2016
SRR1926790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866641
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18210.2101216074476052No Hit
CTGTAGGACGTGGAATATGGCAAGA16970.19581349139955298No Hit
GTATCAACGCAGAGTACTTTTTTTT15880.18323619584118453No Hit
GTCCTAAAGTGTGTATTTCTCATTT13890.1602739773447137No Hit
CTTTAGGACGTGAAATATGGCGAGG13520.15600462013682712No Hit
GTCCTACAGTGTGCATTTCTCATTT10820.12484985132251994No Hit
GGTATCAACGCAGAGTACTTTTTTT9230.10650315413187238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAC903.5191988E-813.7550583
ATCTTAT603.9720253E-412.7102261
GACTAAG603.9720253E-412.7102261
TAGAACC1051.9233994E-812.6969774
GTCTAGG1301.8553692E-1012.4657991
CTAGGAC1650.012.1198423
TGTGCGT550.00309678912.07508410
TCTAACC657.8579393E-411.7202863
TGTAGGA9900.011.3615142
TCTAGGA1850.011.3354912
TAGGACC4550.011.3017054
TATACAA855.188412E-511.2032155
GTCCTAC9200.011.1905261
GTCCTAA3850.011.1420821
AATACTA600.005768532411.1098545
GTAGGAC10050.011.0861653
GACAGTC600.005916906511.072027
TTATACA1552.9831426E-1011.0586584
CTGTAGG9750.011.04812051
GGACGTG8400.010.99585156
Produced by FastQC (version 0.11.5)