FastQCFastQC Report
Thu 26 May 2016
SRR1926789_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926789_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16631
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA5593.3611929529192475No Hit
GTACATGGGAAGCAGTGGTATCAAC4982.994408033191029No Hit
CCCATGTACTCTGCGTTGATACCAC4632.7839576694125427No Hit
GAGTACATGGGAAGCAGTGGTATCA2691.6174613673260778No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA2531.521255486741627No Hit
CATGTACTCTGCGTTGATACCACTG1871.1244062293307677No Hit
GCGTTGATACCACTGCTTCCCATGT1530.91996873308881No Hit
ACGCAGAGTACATGGGAAGCAGTGG1370.8237628525043594No Hit
ACTCTGCGTTGATACCACTGCTTCC1230.7395827069929649No Hit
GTATCAACGCAGAGTACATGGGAAG1200.7215441043833805No Hit
GGTATCAACGCAGAGTACATGGGAA1170.703505501773796No Hit
TATCAACGCAGAGTACATGGGAAGC930.5591966808971198No Hit
GCTTCCCATGTACTCTGCGTTGATA890.5351452107510072No Hit
GTGGTATCAACGCAGAGTACATGGG810.4870422704587818No Hit
GTACTCTGCGTTGATACCACTGCTT740.4449521977030846No Hit
CAGTGGTATCAACGCAGAGTACATG730.4389393301665564No Hit
GAACAAAAAAAAAAAAAAAAAAAAA720.43292646263002826No Hit
GTATCAACGCAGAGTACTTTTTTTT660.3968492574108593No Hit
ATACCACTGCTTCCCATGTACTCTG620.37279778726474655No Hit
GATACCACTGCTTCCCATGTACTCT570.3427334495821057No Hit
GGGAAGCAGTGGTATCAACGCAGAG550.33070771450904934No Hit
GCAGTGGTATCAACGCAGAGTACAT540.32469484697252116No Hit
CTTCCCATGTACTCTGCGTTGATAC540.32469484697252116No Hit
CTGCTTCCCATGTACTCTGCGTTGA530.318681979435993No Hit
ACATGGGAAGCAGTGGTATCAACGC500.3006433768264085No Hit
AAACAAAAAAAAAAAAAAAAAAAAA500.3006433768264085No Hit
CATGGGAAGCAGTGGTATCAACGCA480.2886176417533522No Hit
CCACTGCTTCCCATGTACTCTGCGT470.282604774216824No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA440.2645661716072395No Hit
GGTATCAACGCAGAGTACTTTTTTT430.2585533040707113No Hit
GCAGAGTACATGGGAAGCAGTGGTA410.24652756899765496No Hit
TATCAACGCAGAGTACTTTTTTTTT400.24051470146112683No Hit
AAGCAGTGGTATCAACGCAGAGTAC390.2345018339245986No Hit
GTTGATACCACTGCTTCCCATGTAC380.22848896638807048No Hit
ATGGGAAGCAGTGGTATCAACGCAG330.19842462870542965No Hit
TACCACTGCTTCCCATGTACTCTGC320.19241176116890144No Hit
GAAAACAAAAAAAAAAAAAAAAAAA290.17437315855931693No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA280.16836029102278877No Hit
CCATGTACTCTGCGTTGATACCACT270.16234742348626058No Hit
CTGCGTTGATACCACTGCTTCCCAT270.16234742348626058No Hit
AAAAAGTACTCTGCGTTGATACCAC250.15032168841320426No Hit
ACGCAGAGTACTTTTTTTTTTTTTT250.15032168841320426No Hit
TTCCCATGTACTCTGCGTTGATACC240.1443088208766761No Hit
ACACAAAAAAAAAAAAAAAAAAAAA230.13829595334014794No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA230.13829595334014794No Hit
ATCAACGCAGAGTACATGGGAAGCA230.13829595334014794No Hit
GTACATGGTAAGCAGTGGTATCAAC230.13829595334014794No Hit
TGATACCACTGCTTCCCATGTACTC190.11424448319403524No Hit
ACCATGTACTCTGCGTTGATACCAC180.10823161565750707No Hit
GTACATGGGTAAGCAGTGGTATCAA180.10823161565750707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAAA503.9719143E-617.129911
CAGTGGT1351.8189894E-1112.65060313
AGTGGTA1403.45608E-1112.19879514
GTGGTAT1456.366463E-1111.77814815
TGGTATC1552.0736479E-1011.01826716
GAAGCAG1406.031769E-910.8433739
GGAAGCA1354.0907253E-810.57401858
AAGCAGT1656.166374E-1010.350492510
GGGAAGC1406.983828E-810.1963747
TGGGAAG1406.983828E-810.1963746
AGCAGTG1701.038643E-910.04606611
CATGGGA1552.8157956E-89.8236044
GCAGTGG1751.718945E-99.75903612
ATGGGAA1501.9171603E-79.5166165
GGTATCA1907.1140676E-98.98858617
GTATCAA1907.134622E-88.48922118
TATCAAC2001.634271E-78.06475819
GTACATG2551.7007551E-97.8372131
TACATGG2457.052222E-97.76866632
ACATGGG2251.2050077E-77.6132933
Produced by FastQC (version 0.11.5)