##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926789_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16631
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.85869761289159	31.0	26.0	31.0	2.0	34.0
2	26.125248030785883	31.0	26.0	31.0	2.0	34.0
3	26.06842643256569	31.0	26.0	31.0	2.0	34.0
4	29.297216042330586	35.0	28.0	37.0	2.0	37.0
5	29.661836329745654	35.0	30.0	37.0	2.0	37.0
6	28.827671216403104	35.0	28.0	37.0	2.0	37.0
7	28.99783536768685	35.0	28.0	37.0	2.0	37.0
8	28.683903553604715	35.0	27.0	37.0	2.0	37.0
9	30.44134447718117	37.0	28.0	39.0	2.0	39.0
10	30.697432505561903	37.0	30.0	39.0	2.0	39.0
11	30.705429619385484	37.0	30.0	39.0	2.0	39.0
12	30.782815224580602	37.0	30.0	39.0	2.0	39.0
13	30.731044435091096	37.0	30.0	39.0	2.0	39.0
14	31.79087246707955	38.0	31.0	40.0	2.0	41.0
15	31.25981600625338	38.0	30.0	40.0	2.0	41.0
16	31.236125308159462	38.0	30.0	40.0	2.0	41.0
17	31.46323131501413	38.0	31.0	40.0	2.0	41.0
18	31.39582706992965	38.0	30.0	40.0	2.0	41.0
19	30.755937706692322	38.0	29.0	40.0	2.0	41.0
20	30.92291503818171	38.0	30.0	40.0	2.0	41.0
21	30.866273825987612	38.0	30.0	40.0	2.0	41.0
22	30.95959353015453	38.0	30.0	40.0	2.0	41.0
23	30.914436894955205	38.0	30.0	40.0	2.0	41.0
24	29.71811676988756	37.0	25.0	40.0	2.0	41.0
25	30.221393782694967	38.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.9690129299687165
1101	2	3.9430222298373643
1101	3	-1.0522058408814523
1101	4	1.1793887696301724
1101	5	2.2707431407130336
1101	6	-1.71282321237436
1101	7	1.0947967021861658
1101	8	0.9805224886176873
1101	9	2.06604276429751
1101	10	-6.27921844918918
1101	11	-1.5701742621976145
1101	12	2.395341963905448
1101	13	1.9920415214144285
1101	14	3.057039784818951
1101	15	1.2771308653058782
1101	16	3.610217610738758
1101	17	2.8975886730280997
1101	18	4.846853414018618
1101	19	4.848098294968278
1101	20	5.596266688030706
1101	21	5.216686531388245
1101	22	5.443616874528924
1101	23	5.779530027887908
1101	24	4.841823397995977
1101	25	5.5157510987707035
1102	1	1.575073536029322
1102	2	1.8975676843828175
1102	3	-0.828720992396601
1102	4	0.44454028478168794
1102	5	0.721500716470608
1102	6	-1.0158535154046646
1102	7	1.000099732489197
1102	8	0.31006794316314057
1102	9	0.9524064006611432
1102	10	0.7965391265683976
1102	11	0.05482573780238553
1102	12	0.06200863057211592
1102	13	0.8935566729295807
1102	14	0.7918882696674352
1102	15	1.5650096531846671
1102	16	1.5117327622539136
1102	17	1.5945583699978023
1102	18	0.11958068674589128
1102	19	0.760977082847063
1102	20	1.7364182031822217
1102	21	1.1409289556306703
1102	22	1.2163441472561978
1102	23	0.9916512400091229
1102	24	1.815308246480825
1102	25	2.2771147351343366
1103	1	2.589566289652513
1103	2	2.462785075687165
1103	3	2.6078732105019498
1103	4	2.295989560144008
1103	5	0.4407036150213308
1103	6	1.411682716479394
1103	7	3.175824370170357
1103	8	2.652459247510965
1103	9	3.6154498789220106
1103	10	3.1081333294669484
1103	11	3.0385213899762995
1103	12	2.9170810943402294
1103	13	2.7450059482918974
1103	14	3.766525950826857
1103	15	3.2968937111556826
1103	16	4.145790733268406
1103	17	4.10905112362099
1103	18	4.532624165006759
1103	19	2.3461220103832936
1103	20	4.191128348109753
1103	21	4.394552144036471
1103	22	5.216344147256198
1103	23	4.846723703777236
1103	24	5.393206797205462
1103	25	4.065158213395204
1104	1	1.7634793331307712
1104	2	2.2888720322089036
1104	3	1.8252645148497741
1104	4	3.1220765166657465
1104	5	3.6580949193691588
1104	6	1.3682044556098276
1104	7	4.001911326692092
1104	8	4.782894030119664
1104	9	4.0502324876176665
1104	10	4.803785503379988
1104	11	1.3863474769328192
1104	12	1.395341963905448
1104	13	2.3537016004658113
1104	14	3.0708737769138175
1104	15	3.992545885068722
1104	16	2.6240516028336245
1104	17	2.6307902540557713
1104	18	2.5761024258763285
1104	19	2.693948097339817
1104	20	3.1476500872401907
1104	21	3.351073883166901
1104	22	3.3033006689953304
1104	23	2.6728106602989783
1104	24	2.8714676667706804
1104	25	2.891245169916946
1105	1	-0.030987070031283537
1105	2	1.7612040480191808
1105	3	0.9477941591185477
1105	4	1.7702978605392659
1105	5	0.5434704134403034
1105	6	0.24172224217109317
1105	7	3.276614884004349
1105	8	2.798704306799504
1105	9	1.2933154915702332
1105	10	1.4025997326290032
1105	11	1.0207348287114755
1105	12	0.486251054814538
1105	13	1.9920415214144285
1105	14	1.8297670575462242
1105	15	2.958949047124058
1105	16	2.246581247102398
1105	17	3.21577049120992
1105	18	4.301398868564071
1105	19	0.9390073858773675
1105	20	3.641721233485253
1105	21	2.534868349570065
1105	22	3.4436168745289244
1105	23	2.7795300278879083
1105	24	-1.6127220565494795
1105	25	0.9702965533161532
1106	1	0.8504358548699038
1106	2	0.4193068148175989
1106	3	0.7383079931106451
1106	4	1.4264243427527035
1106	5	0.09287752806481109
1106	6	1.6290740208272219
1106	7	-2.0850451950470372
1106	8	0.8263722909892266
1106	9	0.2676237919654909
1106	10	1.4124811555539054
1106	11	0.3428692160632565
1106	12	0.5692550073837097
1106	13	1.6580494265527719
1106	14	-0.7117349187383581
1106	15	2.036024145938285
1106	16	0.9718776897901407
1106	17	1.7612250366644666
1106	18	0.9674067737024146
1106	19	1.1722089669050355
1106	20	2.669389217674972
1106	21	1.5684651875147289
1106	22	0.12938762551706873
1106	23	-0.28371107883145896
1106	24	-4.867662768011929
1106	25	-1.717450482256968
1107	1	-1.6782597973040119
1107	2	-2.622432315617182
1107	3	-3.2703876590632674
1107	4	-2.1787930485516434
1107	5	-3.7401659501960616
1107	6	-3.6491868487379975
1107	7	-6.60156693417747
1107	8	-4.484932056836861
1107	9	-4.2175935993388585
1107	10	-4.290127540098272
1107	11	-2.7701742621976138
1107	12	-4.0046580360945505
1107	13	-4.369776660403751
1107	14	-4.544778396999231
1107	15	-6.588323680148669
1107	16	-4.111600571079421
1107	17	-4.838774963335535
1107	18	-3.860419313254109
1107	19	-4.617356250486271
1107	20	-2.721915130151114
1107	21	-2.4924043777026625
1107	22	-3.1036558527438025
1107	23	-4.155015426657545
1107	24	-4.116358420185843
1107	25	-4.607885264865665
1108	1	-4.091593130637342
1108	2	-4.65798787117274
1108	3	-3.388906177581788
1108	4	-4.805459715218312
1108	5	-1.9683140983442087
1108	6	-5.738075737626886
1108	7	-4.671196563807101
1108	8	-3.5278950197998213
1108	9	-3.4087047104499675
1108	10	-2.9997571697279
1108	11	-3.7553594473828014
1108	12	-3.975028406464922
1108	13	-4.282369252996343
1108	14	-4.772926545147378
1108	15	-5.564619976444966
1108	16	-3.6641931636720138
1108	17	-4.498034222594793
1108	18	-4.787826720661517
1108	19	-3.5492081023381203
1108	20	-7.129322537558522
1108	21	-5.025737711035994
1108	22	-4.598470667558615
1108	23	-4.897237648879766
1108	24	-4.66154360537103
1108	25	-3.7367741537545527
1109	1	0.8343327952885815
1109	2	0.7123824991976306
1109	3	-2.203720992396601
1109	4	-0.10175601151460612
1109	5	-0.6720178020479111
1109	6	-1.0343720339231837
1109	7	-0.6711965638071007
1109	8	-0.8982653901701916
1109	9	-1.2235195252647841
1109	10	-0.6293867993575297
1109	11	-1.1998038918272442
1109	12	-1.419472850909365
1109	13	-0.986072956700049
1109	14	-0.6988524710733017
1109	15	-2.046101457926447
1109	16	-0.6271561266349792
1109	17	-1.016552741113312
1109	18	-1.750789683624479
1109	19	-1.4751340282640477
1109	20	-1.0552484634844461
1109	21	-1.3220340073322916
1109	22	-1.7836558527438022
1109	23	-1.489830241472358
1109	24	-3.1430250868525107
1109	25	-3.1812185981989956
1110	1	0.32174020269598813
1110	2	0.37756768438281796
1110	3	1.8896123409367327
1110	4	1.5812069514483547
1110	5	2.379834049803943
1110	6	-0.48918684873799734
1110	7	0.5584330658225305
1110	8	0.8750679431631418
1110	9	-0.8575935993388555
1110	10	1.4298724599017305
1110	11	-0.8501742621976156
1110	12	-0.2446580360945525
1110	13	-1.6497766604037523
1110	14	0.09522160300076976
1110	15	-1.748323680148669
1110	16	-0.6316005710794208
1110	17	-0.758774963335533
1110	18	-0.34041931325410957
1110	19	-0.417356250486268
1110	20	-0.601915130151113
1110	21	-0.09240437770266041
1110	22	-0.3036558527438018
1110	23	-0.19501542665754457
1110	24	-2.1163584201858434
1110	25	-1.367885264865663
1111	1	-0.46196350100771255
1111	2	-0.880210093394961
1111	3	0.7592419705663609
1111	4	-1.1758300855886823
1111	5	0.32798219795208894
1111	6	0.5582205586694116
1111	7	-1.041566934177471
1111	8	0.6943272024224001
1111	9	0.6653693636241051
1111	10	-1.4071645771353083
1111	11	-0.19980389182724423
1111	12	-0.64169507313159
1111	13	-0.6527396233667133
1111	14	0.48633271411188517
1111	15	-2.046101457926447
1111	16	-1.2197487192275709
1111	17	-0.6091453337059036
1111	18	0.4714325385977425
1111	19	1.339680786550769
1111	20	-0.018211426447411583
1111	21	0.7150030297047465
1111	22	1.1793071102191597
1111	23	1.1397993881572681
1111	24	1.671789727962306
1111	25	0.7447073277269283
1112	1	0.5631687741245628
1112	2	0.7904248272399599
1112	3	-1.8703876590632689
1112	4	1.1826355228769287
1112	5	1.7869769069467978
1112	6	1.1150988655477185
1112	7	1.7441473515368138
1112	8	2.6136393717345676
1112	9	1.3809778292325703
1112	10	1.4155867456160145
1112	11	0.7869685949452432
1112	12	1.2881991067625904
1112	13	1.8102233395962486
1112	14	2.589507317286486
1112	15	3.767390605565616
1112	16	2.9641137146348626
1112	17	2.225510750950182
1112	18	1.5124378296030372
1112	19	2.409786606656585
1112	20	1.8495134412774554
1112	21	2.343309908011623
1112	22	3.0020584329704825
1112	23	1.932127430485309
1112	24	3.416498722671303
1112	25	2.9021147351343366
1113	1	-2.827225314545391
1113	2	-2.930018522513734
1113	3	-4.8359049004425785
1113	4	-1.3112068416550926
1113	5	-1.1305107777822663
1113	6	-1.4009109866690324
1113	7	-0.7312221065912645
1113	8	-1.2201044706299626
1113	9	-2.9182832545112696
1113	10	-1.990817195270683
1113	11	-2.742588055301063
1113	12	-2.604658036094552
1113	13	-2.4483973500589222
1113	14	-3.2109852935509515
1113	15	-3.440737473252117
1113	16	-2.4509109159070093
1113	17	-2.58360254954243
1113	18	-3.5873158649782475
1113	19	-2.6628734918655788
1113	20	-3.377087543944217
1113	21	-3.3820595501164554
1113	22	-2.88710412860587
1113	23	-2.399153357692029
1113	24	-1.2004963512203268
1113	25	-1.7789197476242826
1114	1	0.8279470992477123
1114	2	1.48377458093454
1114	3	0.9571985478332827
1114	4	0.2060345376552526
1114	5	-0.6822349157133019
1114	6	1.4611579788482096
1114	7	1.5446399623742515
1114	8	1.0557575983355534
1114	9	1.6334408834197625
1114	10	1.733320735763801
1114	11	1.6022395309058304
1114	12	0.6367212742502772
1114	13	0.3102233395962486
1114	14	1.4097043616214613
1114	15	-1.440737473252117
1114	16	0.8594339116791971
1114	17	0.4508802090782602
1114	18	1.3092358591596849
1114	19	2.3371265081344212
1114	20	2.3125676284695764
1114	21	2.583457691262854
1114	22	2.250826905876888
1114	23	-0.675015426657545
1114	24	2.7305381315382924
1114	25	1.2555630109964042
1115	1	-1.4368312258754372
1115	2	1.5047105415256752
1115	3	1.951040912365304
1115	4	3.111206951448356
1115	5	4.17983404980394
1115	6	5.472241722690573
1115	7	4.244147351536814
1115	8	4.75649651459171
1115	9	4.738120686375428
1115	10	2.951301031330299
1115	11	4.28696859494524
1115	12	4.7167705353340175
1115	13	4.988794768167679
1115	14	4.19665017442934
1115	15	4.695962034137047
1115	16	4.964113714634863
1115	17	5.118367893807321
1115	18	5.333866401031607
1115	19	3.552643749513731
1115	20	4.88522772699174
1115	21	5.164738479440192
1115	22	5.216344147256198
1115	23	4.03927028762817
1115	24	3.666498722671303
1115	25	4.759257592277191
1116	1	-3.4619635010077126
1116	2	-4.324654537839404
1116	3	-3.907424696100307
1116	4	-6.509163418922014
1116	5	-4.597943727973838
1116	6	-3.775112774663924
1116	7	-3.6711965638071007
1116	8	-6.268635760540562
1116	9	-4.593889895635154
1116	10	-5.110868280839011
1116	11	-3.644248336271687
1116	12	-4.493546924983441
1116	13	-3.763850734477824
1116	14	-4.143296915517748
1116	15	-6.305360717185707
1116	16	-3.701230200709052
1116	17	-4.5721082966688655
1116	18	-4.417456350291147
1116	19	-2.660319213449231
1116	20	-4.203396611632595
1116	21	-2.618330303628589
1116	22	-4.302174371262321
1116	23	-4.860200611842732
1116	24	-2.7726547164821405
1116	25	-3.2182556352360336
1201	1	4.24174020269599
1201	2	2.977567684382816
1201	3	2.6496123409367307
1201	4	3.181206951448356
1201	5	2.939834049803938
1201	6	2.070813151262005
1201	7	2.998433065822528
1201	8	3.1150679431631403
1201	9	3.8224064006611407
1201	10	3.3098724599017295
1201	11	3.2298257378023827
1201	12	3.115341963905447
1201	13	3.390223339596247
1201	14	3.7352216030007703
1201	15	3.0116763198513326
1201	16	1.928399428920578
1201	17	2.641225036664469
1201	18	2.6995806867458896
1201	19	1.9026437495137323
1201	20	3.1180848698488894
1201	21	2.987595622297338
1201	22	3.536344147256198
1201	23	3.564984573342457
1201	24	2.323641579814158
1201	25	3.752114735134338
1202	1	1.2871947481505366
1202	2	0.26120404801918085
1202	3	-1.5067512954269056
1202	4	0.17938876963017236
1202	5	0.4980158679857567
1202	6	0.33263133308018666
1202	7	0.5038876112770758
1202	8	1.0714315795267773
1202	9	0.7024064006611432
1202	10	0.1298724599017298
1202	11	0.11164391962056897
1202	12	-0.4228398542763685
1202	13	-1.1897766604037514
1202	14	0.2388579666371342
1202	15	0.32258541076042135
1202	16	-0.7534187528976055
1202	17	-0.14786587242644345
1202	18	-0.8804193132541087
1202	19	-0.5609926141226325
1202	20	0.5962666880307026
1202	21	1.3075956222973382
1202	22	-0.0563831254710756
1202	23	0.23407548243336507
1202	24	-2.839994783822206
1202	25	-1.6206125375929368
1203	1	-1.6782597973040119
1203	2	-1.6624323156171812
1203	3	-0.8303876590632697
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2211	13	-2.7268136974407895
2211	14	-1.6988524710733017
2211	15	-0.6757310875560769
2211	16	-2.8493783488572006
2211	17	-3.1647008892614608
2211	18	-3.269308202142998
2211	19	-4.80846736159738
2211	20	-4.536729944965927
2211	21	-4.062774748073032
2211	22	-4.265137334225283
2211	23	-4.600941352583472
2211	24	-2.7726547164821405
2211	25	-3.7367741537545527
2212	1	-1.6915931306373437
2212	2	-2.202432315617184
2212	3	-4.303720992396602
2212	4	-3.8387930485516435
2212	5	-1.186832616862727
2212	6	-0.34918684873799677
2212	7	0.2250997324891948
2212	8	0.13506794316313986
2212	9	-0.7309269326721903
2212	10	-0.8367942067649352
2212	11	-0.20350759553094733
2212	12	-0.37132470276121765
2212	13	0.17689000626291573
2212	14	-0.791445063665897
2212	15	-2.0683236801486693
2212	16	-0.7049339044127549
2212	17	-2.838774963335535
2212	18	-2.6804193132541094
2212	19	-1.7973562504862706
2212	20	-4.521915130151115
2212	21	-3.6257377110359954
2212	22	-3.1169891860771344
2212	23	-2.808348759990878
2212	24	-1.34302508685251
2212	25	-2.747885264865662
2213	1	-0.1868312258754372
2213	2	0.3261391129542446
2213	3	0.9153266266510158
2213	4	-3.8173644771230713
2213	5	-1.3201659501960599
2213	6	-3.8849011344522815
2213	7	-0.3272812198917556
2213	8	-1.4577891996940018
2213	9	-1.4047364564817144
2213	10	0.7013010313303027
2213	11	-0.4987456907690415
2213	12	0.466770535334021
2213	13	0.45308048245339094
2213	14	0.732364460143625
2213	15	-0.2326093944343839
2213	16	0.606970857492005
2213	17	0.654082179521609
2213	18	1.5838664010316066
2213	19	1.1597866066565885
2213	20	0.528084869848886
2213	21	1.3790241937259076
2213	22	0.25205843297048247
2213	23	0.824984573342455
2213	24	-0.3335012773287005
2213	25	1.0092575922771942
2214	1	-1.2065356593729746
2214	2	-1.930018522513734
2214	3	0.3709916512815603
2214	4	-1.4491378761378506
2214	5	-0.6132693984719211
2214	6	-1.4353937452897192
2214	7	-0.0070841755567805365
2214	8	-4.495966539595479
2214	9	-2.470007392442305
2214	10	-2.473575815960338
2214	11	-1.6046570208183049
2214	12	-6.121899415404897
2214	13	-3.3449490741968546
2214	14	-2.1420197763095743
2214	15	-3.440737473252117
2214	16	-2.416428157286319
2214	17	-4.031878411611395
2214	18	-3.0011089684265215
2214	19	-1.1456321125552336
2214	20	-3.6874323715304236
2214	21	-2.6579216190819714
2214	22	-1.4733110251575958
2214	23	-1.0198430128644418
2214	24	-1.269461868461704
2214	25	-2.744436989003596
2215	1	-1.4034210876265902
2215	2	-1.3604968317462145
2215	3	-0.8381295945471408
2215	4	-0.9452446614548684
2215	5	0.11531792077168035
2215	6	-0.9137029777702566
2215	7	-1.1060830632097307
2215	8	-1.9197707665142794
2215	9	-2.458883921919501
2215	10	-2.0152888304208503
2215	11	-3.0540452299395504
2215	12	-1.0562709393203598
2215	13	-1.5930024668553635
2215	14	-1.3021977518379373
2215	15	-1.7844527124067326
2215	16	-1.7490199259181303
2215	17	-1.5290975439806935
2215	18	-2.2513870551895927
2215	19	-1.1167110891959453
2215	20	-3.721915130151114
2215	21	-2.982726958347822
2215	22	-4.17075262693735
2215	23	-2.675015426657545
2215	24	-1.8311971298632628
2215	25	-2.2027239745430833
2216	1	0.7244988233856446
2216	2	-1.8610530052723533
2216	3	-1.4910773142356817
2216	4	-2.000862014068886
2216	5	0.2487995670453209
2216	6	-0.12504891770351279
2216	7	-5.351911761763677
2216	8	-6.806311367181689
2216	9	-3.470007392442305
2216	10	-0.7839206435465478
2216	11	-0.9150018484045113
2216	12	-0.4667270016117939
2216	13	-1.5518456259209934
2216	14	-1.5902956383785387
2216	15	-3.164875404286601
2216	16	-3.0026350538380413
2216	17	-2.652568066783811
2216	18	-2.1390400029092795
2216	19	-2.2835631470379916
2216	20	-3.4115703025649076
2216	21	-4.451025067357833
2216	22	-4.335379990674838
2216	23	-3.1577740473472
2216	24	-3.0970480753582557
2216	25	-3.330643885555318
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1788.0
3	0.0
4	10.0
5	68.0
6	139.0
7	117.0
8	61.0
9	65.0
10	46.0
11	59.0
12	58.0
13	64.0
14	61.0
15	47.0
16	63.0
17	57.0
18	51.0
19	80.0
20	101.0
21	166.0
22	161.0
23	175.0
24	214.0
25	196.0
26	221.0
27	240.0
28	233.0
29	246.0
30	279.0
31	365.0
32	468.0
33	632.0
34	764.0
35	1146.0
36	1858.0
37	2841.0
38	3491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.711635750421586	21.30209588051072	9.75668513611178	22.229583232955914
2	14.517974348166435	27.789486361172997	30.968868549406874	26.723670741253684
3	21.948575901728187	34.09405672306858	22.093093273920637	21.86427410128259
4	12.987481945113144	24.32595089070775	27.238805970149254	35.44776119402985
5	12.551118595140725	43.67332210728891	28.897041135434208	14.878518162136157
6	30.424585037286505	35.8672119316815	16.32788068318499	17.380322347847006
7	23.885004821600774	38.67526518804243	18.297974927675988	19.141755062680808
8	26.355140186915886	36.68375037684655	18.82423876997287	18.1368706662647
9	26.329083473658887	23.201828241520328	16.15347606446957	34.31561222035121
10	17.234119345524544	35.37656400384985	25.48724735322425	21.902069297401347
11	27.788802694088638	33.45961873834867	15.61128149618137	23.140297071381323
12	23.08756314649988	36.25811883569882	22.438056290594176	18.21626172720712
13	28.173672499849662	28.997534427806844	18.828552528714894	24.0002405436286
14	21.133028626413278	33.130863603560265	19.834014914601877	25.902092855424584
15	29.9236273979193	30.26640206867521	18.233207047928317	21.57676348547718
16	19.659610295886456	34.47798893432764	24.945874428674525	20.91652634111138
17	23.514553764734185	35.12148183786385	21.638200625451045	19.725763771950927
18	23.76571050574298	30.945937819472007	24.16260749293403	21.125744181850983
19	22.840991099350493	35.03728650469088	24.560981477026704	17.560740918931923
20	23.59131637500752	32.01034337602982	23.164351434241386	21.23398881472127
21	20.933309278970473	34.03090985627518	24.03030849720368	21.005472367550666
22	22.364543869144267	33.62799927836912	18.71429430512959	25.293162547357028
23	19.274717344238635	34.14722155400529	22.93721433726245	23.640846764493624
24	21.342233447591557	37.46467015454928	20.55445306392447	20.63864333393469
25	21.871429430512958	31.439052258103313	19.55018341451681	27.139334896866917
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	625.0
1	625.0
2	546.0
3	467.0
4	467.0
5	467.0
6	398.0
7	329.0
8	329.0
9	329.0
10	256.0
11	183.0
12	183.0
13	183.0
14	170.0
15	157.0
16	157.0
17	157.0
18	171.5
19	186.0
20	186.0
21	186.0
22	227.5
23	269.0
24	269.0
25	269.0
26	334.0
27	399.0
28	399.0
29	399.0
30	466.0
31	533.0
32	533.0
33	533.0
34	579.0
35	625.0
36	625.0
37	625.0
38	682.0
39	739.0
40	739.0
41	739.0
42	937.5
43	1136.0
44	1136.0
45	1136.0
46	2238.5
47	3341.0
48	3341.0
49	3341.0
50	2971.5
51	2602.0
52	2602.0
53	2602.0
54	2072.0
55	1542.0
56	1542.0
57	1542.0
58	1345.5
59	1149.0
60	1149.0
61	1149.0
62	1040.5
63	932.0
64	932.0
65	932.0
66	809.0
67	686.0
68	686.0
69	686.0
70	553.5
71	421.0
72	421.0
73	421.0
74	301.0
75	181.0
76	181.0
77	181.0
78	135.5
79	90.0
80	90.0
81	90.0
82	60.0
83	30.0
84	30.0
85	30.0
86	18.5
87	7.0
88	7.0
89	7.0
90	4.5
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16234742348626058
2	0.1443088208766761
3	0.1443088208766761
4	0.09019301304792256
5	0.018038602609584512
6	0.018038602609584512
7	0.2345018339245986
8	0.2765919066802959
9	0.018038602609584512
10	0.04209007275569719
11	0.01202573507305634
12	0.018038602609584512
13	0.01202573507305634
14	0.018038602609584512
15	0.01202573507305634
16	0.018038602609584512
17	0.018038602609584512
18	0.01202573507305634
19	0.018038602609584512
20	0.01202573507305634
21	0.01202573507305634
22	0.01202573507305634
23	0.018038602609584512
24	0.01202573507305634
25	0.01202573507305634
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	16631.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.14941975828272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.16105214582372	60.693884913715344
2	4.577757268112598	5.964764596235945
3	0.8214120904476233	1.6054356322530212
4	0.3784033225657591	0.9861102759906198
5	0.18458698661744347	0.601286753652817
6	0.09229349330872173	0.36077205219169023
7	0.07383479464697738	0.33672058204557753
8	0.07383479464697738	0.38482352233780287
9	0.027688047992616517	0.16234742348626058
>10	0.3876326718966313	6.283446575671937
>50	0.11998154130133826	5.309362034754375
>100	0.09229349330872173	13.949852684745355
>500	0.009229349330872173	3.3611929529192475
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	559	3.3611929529192475	No Hit
GTACATGGGAAGCAGTGGTATCAAC	498	2.994408033191029	No Hit
CCCATGTACTCTGCGTTGATACCAC	463	2.7839576694125427	No Hit
GAGTACATGGGAAGCAGTGGTATCA	269	1.6174613673260778	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	253	1.521255486741627	No Hit
CATGTACTCTGCGTTGATACCACTG	187	1.1244062293307677	No Hit
GCGTTGATACCACTGCTTCCCATGT	153	0.91996873308881	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	137	0.8237628525043594	No Hit
ACTCTGCGTTGATACCACTGCTTCC	123	0.7395827069929649	No Hit
GTATCAACGCAGAGTACATGGGAAG	120	0.7215441043833805	No Hit
GGTATCAACGCAGAGTACATGGGAA	117	0.703505501773796	No Hit
TATCAACGCAGAGTACATGGGAAGC	93	0.5591966808971198	No Hit
GCTTCCCATGTACTCTGCGTTGATA	89	0.5351452107510072	No Hit
GTGGTATCAACGCAGAGTACATGGG	81	0.4870422704587818	No Hit
GTACTCTGCGTTGATACCACTGCTT	74	0.4449521977030846	No Hit
CAGTGGTATCAACGCAGAGTACATG	73	0.4389393301665564	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	72	0.43292646263002826	No Hit
GTATCAACGCAGAGTACTTTTTTTT	66	0.3968492574108593	No Hit
ATACCACTGCTTCCCATGTACTCTG	62	0.37279778726474655	No Hit
GATACCACTGCTTCCCATGTACTCT	57	0.3427334495821057	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	55	0.33070771450904934	No Hit
GCAGTGGTATCAACGCAGAGTACAT	54	0.32469484697252116	No Hit
CTTCCCATGTACTCTGCGTTGATAC	54	0.32469484697252116	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	53	0.318681979435993	No Hit
ACATGGGAAGCAGTGGTATCAACGC	50	0.3006433768264085	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	50	0.3006433768264085	No Hit
CATGGGAAGCAGTGGTATCAACGCA	48	0.2886176417533522	No Hit
CCACTGCTTCCCATGTACTCTGCGT	47	0.282604774216824	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA	44	0.2645661716072395	No Hit
GGTATCAACGCAGAGTACTTTTTTT	43	0.2585533040707113	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	41	0.24652756899765496	No Hit
TATCAACGCAGAGTACTTTTTTTTT	40	0.24051470146112683	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	39	0.2345018339245986	No Hit
GTTGATACCACTGCTTCCCATGTAC	38	0.22848896638807048	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	33	0.19842462870542965	No Hit
TACCACTGCTTCCCATGTACTCTGC	32	0.19241176116890144	No Hit
GAAAACAAAAAAAAAAAAAAAAAAA	29	0.17437315855931693	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA	28	0.16836029102278877	No Hit
CCATGTACTCTGCGTTGATACCACT	27	0.16234742348626058	No Hit
CTGCGTTGATACCACTGCTTCCCAT	27	0.16234742348626058	No Hit
AAAAAGTACTCTGCGTTGATACCAC	25	0.15032168841320426	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	25	0.15032168841320426	No Hit
TTCCCATGTACTCTGCGTTGATACC	24	0.1443088208766761	No Hit
ACACAAAAAAAAAAAAAAAAAAAAA	23	0.13829595334014794	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA	23	0.13829595334014794	No Hit
ATCAACGCAGAGTACATGGGAAGCA	23	0.13829595334014794	No Hit
GTACATGGTAAGCAGTGGTATCAAC	23	0.13829595334014794	No Hit
TGATACCACTGCTTCCCATGTACTC	19	0.11424448319403524	No Hit
ACCATGTACTCTGCGTTGATACCAC	18	0.10823161565750707	No Hit
GTACATGGGTAAGCAGTGGTATCAA	18	0.10823161565750707	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAAAAA	50	3.9719143E-6	17.12991	1
CAGTGGT	135	1.8189894E-11	12.650603	13
AGTGGTA	140	3.45608E-11	12.198795	14
GTGGTAT	145	6.366463E-11	11.778148	15
TGGTATC	155	2.0736479E-10	11.018267	16
GAAGCAG	140	6.031769E-9	10.843373	9
GGAAGCA	135	4.0907253E-8	10.5740185	8
AAGCAGT	165	6.166374E-10	10.3504925	10
GGGAAGC	140	6.983828E-8	10.196374	7
TGGGAAG	140	6.983828E-8	10.196374	6
AGCAGTG	170	1.038643E-9	10.046066	11
CATGGGA	155	2.8157956E-8	9.823604	4
GCAGTGG	175	1.718945E-9	9.759036	12
ATGGGAA	150	1.9171603E-7	9.516616	5
GGTATCA	190	7.1140676E-9	8.988586	17
GTATCAA	190	7.134622E-8	8.489221	18
TATCAAC	200	1.634271E-7	8.064758	19
GTACATG	255	1.7007551E-9	7.837213	1
TACATGG	245	7.052222E-9	7.7686663	2
ACATGGG	225	1.2050077E-7	7.613293	3
>>END_MODULE