FastQCFastQC Report
Thu 26 May 2016
SRR1926789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926789_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16631
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC5393.240935602188684No Hit
GTACATGGGAAGCAGTGGTATCAAC5233.1447297216042327No Hit
GTATCAACGCAGAGTACTTTTTTTT4392.639648848535867No Hit
GGTATCAACGCAGAGTACTTTTTTT3672.2067223859058385No Hit
GAGTACATGGGAAGCAGTGGTATCA2641.5873970296434372No Hit
TATCAACGCAGAGTACTTTTTTTTT2611.5693584270338525No Hit
CATGTACTCTGCGTTGATACCACTG2091.2566893151343876No Hit
ACGCAGAGTACATGGGAAGCAGTGG1931.1604834345499369No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1921.1544705670134088No Hit
TATCAACGCAGAGTACATGGGAAGC1681.0101617461367327No Hit
GCGTTGATACCACTGCTTCCCATGT1630.9800974084540918No Hit
GTATCAACGCAGAGTACATGGGAAG1470.8838915278696411No Hit
GGTATCAACGCAGAGTACATGGGAA1430.8598400577235283No Hit
ACTCTGCGTTGATACCACTGCTTCC1300.7816727797486621No Hit
GTACTTTTTTTTTTTTTTTTTTTTT900.5411580782875353No Hit
GAGTACTTTTTTTTTTTTTTTTTTT850.5110937406048944No Hit
GTGGTATCAACGCAGAGTACATGGG840.5050808730683664No Hit
GCTTCCCATGTACTCTGCGTTGATA840.5050808730683664No Hit
CATGGGAAGCAGTGGTATCAACGCA830.49906800553183817No Hit
ATACCACTGCTTCCCATGTACTCTG800.48102940292225366No Hit
GTACTCTGCGTTGATACCACTGCTT740.4449521977030846No Hit
GCAGTGGTATCAACGCAGAGTACAT740.4449521977030846No Hit
ACATGGGAAGCAGTGGTATCAACGC680.40887499248391557No Hit
GATACCACTGCTTCCCATGTACTCT670.40286212494738743No Hit
CAGTGGTATCAACGCAGAGTACATG650.39083638987433106No Hit
GGGAAGCAGTGGTATCAACGCAGAG650.39083638987433106No Hit
CCACTGCTTCCCATGTACTCTGCGT600.36077205219169023No Hit
GCAGAGTACATGGGAAGCAGTGGTA570.3427334495821057No Hit
GCAGAGTACTTTTTTTTTTTTTTTT530.318681979435993No Hit
ATCAACGCAGAGTACTTTTTTTTTT480.2886176417533522No Hit
GTTGATACCACTGCTTCCCATGTAC480.2886176417533522No Hit
AAGCAGTGGTATCAACGCAGAGTAC480.2886176417533522No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA470.282604774216824No Hit
CTGCTTCCCATGTACTCTGCGTTGA470.282604774216824No Hit
GTGGTATCAACGCAGAGTACTTTTT460.2765919066802959No Hit
TACCACTGCTTCCCATGTACTCTGC460.2765919066802959No Hit
CTTCCCATGTACTCTGCGTTGATAC460.2765919066802959No Hit
ATGGGAAGCAGTGGTATCAACGCAG430.2585533040707113No Hit
CCATGTACTCTGCGTTGATACCACT420.2525404365341832No Hit
ATCAACGCAGAGTACATGGGAAGCA370.2224760988515423No Hit
AAAAAGTACTCTGCGTTGATACCAC300.18038602609584511No Hit
TTCCCATGTACTCTGCGTTGATACC300.18038602609584511No Hit
AACGCAGAGTACTTTTTTTTTTTTT250.15032168841320426No Hit
CAACGCAGAGTACTTTTTTTTTTTT240.1443088208766761No Hit
GTACATGGTAAGCAGTGGTATCAAC220.13228308580361975No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA200.12025735073056341No Hit
GGAAGCAGTGGTATCAACGCAGAGT200.12025735073056341No Hit
TCAACGCAGAGTACTTTTTTTTTTT190.11424448319403524No Hit
TGGGAAGCAGTGGTATCAACGCAGA180.10823161565750707No Hit
CCCCATGTACTCTGCGTTGATACCA180.10823161565750707No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG402.4797634E-416.6939414
TACATGG2759.094947E-128.325292
GAAGCAG1854.5486922E-78.2005869
AGCAGTG2103.9863153E-88.12736611
GCAGTGG2155.9459126E-87.938358312
GTACATG2903.274181E-117.89467051
TCAACGC2301.9041181E-87.8803695
ATCAACG2301.9041181E-87.8803694
ACATGGG2801.364242E-107.83593033
AACGCAG2302.1267624E-87.83289677
GGAAGCA1951.0128151E-67.7800438
GGGAAGC1951.0128151E-67.7800437
AGTACTT1355.482089E-47.72601514
GAGTACT1355.482089E-47.72601513
CAACGCA2352.7932401E-87.7127026
AAGCAGT2103.6151505E-77.67584610
AGTGGTA2155.266793E-77.497338314
TGGGAAG2051.9648578E-67.44538076
TGGTATC2207.598701E-77.326944416
GTGGTAT2207.598701E-77.326944415
Produced by FastQC (version 0.11.5)