##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926789_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16631 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.383681077505862 31.0 30.0 33.0 25.0 34.0 2 29.530815946124708 31.0 30.0 33.0 25.0 34.0 3 29.592507967049485 31.0 30.0 34.0 25.0 34.0 4 33.16793939029523 35.0 33.0 37.0 27.0 37.0 5 33.240695087487225 35.0 35.0 37.0 28.0 37.0 6 33.136912993806746 36.0 35.0 37.0 27.0 37.0 7 33.1866995370092 37.0 35.0 37.0 27.0 37.0 8 33.11701040226084 37.0 35.0 37.0 27.0 37.0 9 34.587938187721726 39.0 35.0 39.0 27.0 39.0 10 34.40472611388371 39.0 35.0 39.0 27.0 39.0 11 34.4066502314954 39.0 35.0 39.0 26.0 39.0 12 34.32096686909988 39.0 35.0 39.0 26.0 39.0 13 34.290481630689676 39.0 35.0 39.0 26.0 39.0 14 35.212073838013346 39.0 36.0 41.0 25.0 41.0 15 35.122782755095905 39.0 35.0 41.0 25.0 41.0 16 34.97847393421923 39.0 35.0 41.0 25.0 41.0 17 35.14972040165955 39.0 36.0 41.0 25.0 41.0 18 35.09710781071493 39.0 36.0 41.0 25.0 41.0 19 35.170344537309845 39.0 36.0 41.0 25.0 41.0 20 35.183572845890204 39.0 36.0 41.0 25.0 41.0 21 35.03836209488305 39.0 36.0 41.0 24.0 41.0 22 34.99007876856473 39.0 36.0 41.0 24.0 41.0 23 34.89537610486441 39.0 36.0 41.0 21.0 41.0 24 34.789549636221516 40.0 35.0 41.0 21.0 41.0 25 34.702844086344776 39.0 35.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0019952096816596 1101 2 -0.18218390298063625 1101 3 0.6264929546681977 1101 4 0.980129238923233 1101 5 1.4686487395748458 1101 6 0.010081144905662143 1101 7 0.509141525944699 1101 8 0.6461911636335742 1101 9 -0.4432976411964802 1101 10 0.5825985341063529 1101 11 0.19382779604607947 1101 12 0.6145055894945699 1101 13 0.0696264820344723 1101 14 -0.36423954964614325 1101 15 -0.14599757512708322 1101 16 -0.6084511651997531 1101 17 -0.120871272159782 1101 18 0.447308042532903 1101 19 0.4071731963615264 1101 20 1.083425677181232 1101 21 0.7094029344413286 1101 22 0.7190353002794794 1101 23 1.5680894762985247 1101 24 0.6238917770719752 1101 25 0.7431692076133416 1102 1 0.8694194521059018 1102 2 1.7269070061102738 1102 3 1.933311136486381 1102 4 2.036947420741413 1102 5 1.7754669213930256 1102 6 2.123717508542029 1102 7 2.4485354653386366 1102 8 2.441645709088121 1102 9 2.6286720557732224 1102 10 2.5788106553184704 1102 11 2.5423126445309236 1102 12 2.8985964985854764 1102 13 2.990081027489019 1102 14 2.9615180261114276 1102 15 2.50172969760019 1102 16 3.255185198436614 1102 17 2.7692802429917336 1102 18 3.754126224351083 1102 19 2.9867186509069796 1102 20 3.034183252938803 1102 21 3.4594029344413286 1102 22 3.139489845734026 1102 23 3.0680894762985247 1102 24 3.9079826861628817 1102 25 3.9363510257951617 1103 1 1.6067382926856126 1103 2 1.5095157017624494 1103 3 2.199615484312467 1103 4 2.901077855524022 1103 5 2.106988660523463 1103 6 2.199804465063771 1103 7 2.4557818421502304 1103 8 1.7133848395229023 1103 9 0.35512133113553546 1103 10 1.64583964082572 1103 11 2.0495590213425174 1103 12 2.110553020324609 1103 13 0.941167984010761 1103 14 3.2187644029230214 1103 15 2.170207958469753 1103 16 3.037793894088786 1103 17 2.7565990835714445 1103 18 3.476952311307606 1103 19 3.600849085689589 1103 20 3.470777455837357 1103 21 3.0789681518326333 1103 22 3.2210115848644634 1103 23 2.3941764328202666 1103 24 1.6199392079020143 1103 25 1.9961336344908105 1104 1 2.3893469883377882 1104 2 2.552993962632012 1104 3 2.503963310399424 1104 4 2.6402082903066315 1104 5 3.280901704001721 1104 6 3.1128479433246383 1104 7 2.4992601030198003 1104 8 2.756863100392472 1104 9 3.1377300267877146 1104 10 3.689317901695283 1104 11 3.0060807604729547 1104 12 2.4149008464115624 1104 13 4.202037549228152 1104 14 3.5665904898795446 1104 15 4.083251436730627 1104 16 3.6464895462627 1104 17 3.4957295183540467 1104 18 4.8247783982641295 1104 19 3.6443273465591517 1104 20 3.688168760185185 1104 21 4.296359456180461 1104 22 4.307968106603589 1104 23 4.307219911081134 1104 24 4.359069642684624 1104 25 3.6048292866647245 1105 1 1.5474497551362063 1105 2 0.45417973338300044 1105 3 1.399220227395471 1105 4 2.2528565116505064 1105 5 1.3322851032112055 1105 6 1.6918993267238491 1105 7 -0.21813120132802766 1105 8 -0.2628997454573323 1105 9 0.5112478133489802 1105 10 1.5371439886518061 1105 11 0.9211005233188061 1105 12 1.11450558949457 1105 13 1.296899209307199 1105 14 1.59030590489931 1105 15 0.5358206066910967 1105 16 1.709730652982067 1105 17 2.060946909658398 1105 18 2.0382171334419894 1105 19 1.6799004690887998 1105 20 1.4925165862721386 1105 21 1.8003120253504221 1105 22 2.5826716639158462 1105 23 2.5680894762985247 1105 24 2.8966190497992486 1105 25 2.834078298522435 1106 1 1.2154339448595266 1106 2 -0.9687451678027692 1106 3 0.4604850495298578 1106 4 0.9445561163935849 1106 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19 1.9771032662915928 2209 20 1.0309781247336787 2209 21 -0.8290586040202115 2209 22 -0.6585870773428937 2209 23 -0.6242182160091687 2209 24 -0.813171159990965 2209 25 -0.4771105126663784 2210 1 0.2310861187725699 2210 2 -0.03309299388972775 2210 3 0.44831113648638166 2210 4 0.20194742074141203 2210 5 -0.0495330786069772 2210 6 1.0937175085420279 2210 7 1.961868798671972 2210 8 0.8316457090881215 2210 9 1.7203387224398838 2210 10 1.7971439886518041 2210 11 1.8756459778642594 2210 12 1.4635964985854741 2210 13 2.0350810274890208 2210 14 2.724851359444763 2210 15 -0.4332703023998121 2210 16 1.3751851984366112 2210 17 1.2609469096584007 2210 18 0.16912622435108204 2210 19 1.7817186509069813 2210 20 1.1525165862721352 2210 21 0.009402934441325783 2210 22 1.0244898457340241 2210 23 0.4080894762985281 2210 24 2.652982686162879 2210 25 3.121351025795164 2211 1 -2.866691659005209 2211 2 -3.236055956852688 2211 3 -2.3398370116617677 2211 4 -3.0602748014808085 2211 5 -3.108792337866234 2211 6 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2212 18 -2.204207108982253 2212 19 -1.8716146824263546 2212 20 -1.3074834137278586 2212 21 -1.4905970655586742 2212 22 -1.9021768209326382 2212 23 -1.4652438570348068 2212 24 -1.6670173138371212 2212 25 -1.6719823075381726 2213 1 0.19680040448685432 2213 2 0.2269070061102738 2213 3 0.7368825650578081 2213 4 0.23337599216998228 2213 5 0.9004669213930256 2213 6 0.9808603656848831 2213 7 1.0318687986719723 2213 8 0.8345028519452669 2213 9 1.491767293868456 2213 10 2.037143988651806 2213 11 0.16136026357854405 2213 12 0.9164536414426223 2213 13 0.36508102748901905 2213 14 0.25913707373047856 2213 15 -0.6946988738283792 2213 16 0.8623280555794679 2213 17 0.4895183382298285 2213 18 -0.1208737756489171 2213 19 0.14743293662126433 2213 20 0.06394515770070797 2213 21 0.8522600772984745 2213 22 0.22877556001974142 2213 23 -0.6104819522729059 2213 24 -0.1813030281228336 2213 25 -0.6172204027762689 2214 1 -3.1233966398481208 2214 2 -3.238610235269036 2214 3 -3.2606543807549997 2214 4 -4.182880165465484 2214 5 -2.2288434234345615 2214 6 -2.3159376638717646 2214 7 -2.7870967185694084 2214 8 -2.7393887736704983 2214 9 -3.0796612775601133 2214 10 -2.8594077354861263 2214 11 -2.36573333248057 2214 12 -3.2177828117593528 2214 13 -1.7814706966489098 2214 14 -2.5758382957276496 2214 15 -2.6318909920549842 2214 16 -3.227573422253041 2214 17 -2.3528461937898797 2214 18 -2.3881151549592587 2214 19 -3.207246866334401 2214 20 -4.007483413727861 2214 21 -3.4457694793517746 2214 22 -3.2872342921970095 2214 23 -3.7422553512876817 2214 24 -3.0532242103888407 2214 25 -3.1627869052393223 2215 1 -0.3792364618725905 2215 2 -0.6279317035671461 2215 3 0.09863371713154123 2215 4 0.6780764529994769 2215 5 0.37627337300592956 2215 6 -1.0456373301676471 2215 7 -0.8471634593925437 2215 8 -0.7115800973634947 2215 9 -3.176435471108501 2215 10 -3.188662462961098 2215 11 -3.8985475705228367 2215 12 -2.782855114317748 2215 13 -3.433306069285173 2215 14 -2.1486970276520125 2215 15 -1.4861735282062583 2215 16 -1.3899760918859698 2215 17 -2.2293756709867623 2215 18 -2.096680227261821 2215 19 -2.4286039297381805 2215 20 -1.5881285750181817 2215 21 -2.40350029136512 2215 22 -2.6064778962014543 2215 23 -1.4319105237014753 2215 24 -2.8218560235145382 2215 25 -1.7612296193661265 2216 1 -4.399258708813637 2216 2 -4.031713683544897 2216 3 -5.98479231178948 2216 4 -6.769087062017206 2216 5 -6.608153768262145 2216 6 -5.109041112147626 2216 7 -5.2008898220176825 2216 8 -4.946285325394637 2216 9 -4.700350932732526 2216 10 -5.3766491147964715 2216 11 -5.503664366963328 2216 12 -5.079851777276591 2216 13 -5.781470696648913 2216 14 -5.1620451922793755 2216 15 -5.666373750675671 2216 16 -4.331021698115109 2216 17 -5.318363435169189 2216 18 -5.1812186032351235 2216 19 -5.310695142196469 2216 20 -5.662655827520965 2216 21 -4.376803962110394 2216 22 -5.735510154265974 2216 23 -4.707772592666991 2216 24 -4.32908627935436 2216 25 -4.404166215584148 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 496.0 3 0.0 4 12.0 5 97.0 6 100.0 7 79.0 8 67.0 9 29.0 10 38.0 11 25.0 12 34.0 13 36.0 14 30.0 15 28.0 16 27.0 17 34.0 18 34.0 19 41.0 20 60.0 21 73.0 22 83.0 23 91.0 24 107.0 25 134.0 26 126.0 27 178.0 28 167.0 29 247.0 30 275.0 31 358.0 32 466.0 33 667.0 34 807.0 35 1144.0 36 1671.0 37 2944.0 38 5826.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.7907536720443 18.312063568504698 9.830243197688418 24.06693956176258 2 16.151947504665586 22.78610559267955 33.273132261754256 27.788814640900604 3 22.057850742678454 28.4142161284503 27.10325335257682 22.424679776294425 4 14.352714809692744 19.6801154470567 29.39690938608622 36.57026035716433 5 12.960067356266538 38.182583593937935 32.294924224200145 16.562424825595382 6 32.51674410185241 32.07626862969891 16.00193085138478 19.4050564170639 7 24.263202213400696 35.143750751834474 20.00481174064718 20.588235294117645 8 29.165914967827288 31.342834806663056 19.411870828071443 20.07937939743821 9 28.60665103133081 14.64910698177882 18.972878705875278 37.7713632810151 10 19.6452194828623 28.719182200841853 27.203848466626578 24.431749849669274 11 29.953695351494375 25.557760538817725 17.409345119971135 27.079198989716758 12 25.87336900968072 30.04028621249474 25.759124526486683 18.327220251337863 13 32.44543322710601 20.79850880885094 22.734652155613013 24.02140580843004 14 24.075521616258794 25.23600505080873 25.055619024712882 25.63285430821959 15 33.59591100420926 21.27480457005412 23.487672880336742 21.64161154539988 16 19.800372797787265 26.787324875232997 30.352955324394205 23.059347002585532 17 23.606517948409596 27.881666766881125 26.98574950393843 21.52606578077085 18 24.039444411039625 21.514040045697794 31.687811917503456 22.758703625759125 19 22.188815393866506 26.181599518941674 33.553818400481056 18.075766686710764 20 24.052916416115455 21.797955502104628 32.537582681900176 21.611545399879738 21 20.62785662737551 24.621121000721676 33.41953331729613 21.331489054606685 22 22.74066502314954 23.59449221333654 27.89369250195418 25.77115026155974 23 20.028861764175335 23.107449942877757 32.974565570320486 23.88912272262642 24 21.74854187962239 27.72533221093139 29.853887318862366 20.67223859058385 25 22.3765710505743 21.372301401166638 29.18395573997234 27.067171808286727 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 70.0 1 70.0 2 61.0 3 52.0 4 52.0 5 52.0 6 91.0 7 130.0 8 130.0 9 130.0 10 138.0 11 146.0 12 146.0 13 146.0 14 104.5 15 63.0 16 63.0 17 63.0 18 117.5 19 172.0 20 172.0 21 172.0 22 296.0 23 420.0 24 420.0 25 420.0 26 539.0 27 658.0 28 658.0 29 658.0 30 767.0 31 876.0 32 876.0 33 876.0 34 919.5 35 963.0 36 963.0 37 963.0 38 857.5 39 752.0 40 752.0 41 752.0 42 912.0 43 1072.0 44 1072.0 45 1072.0 46 2282.0 47 3492.0 48 3492.0 49 3492.0 50 3083.0 51 2674.0 52 2674.0 53 2674.0 54 2141.5 55 1609.0 56 1609.0 57 1609.0 58 1346.0 59 1083.0 60 1083.0 61 1083.0 62 1010.5 63 938.0 64 938.0 65 938.0 66 794.5 67 651.0 68 651.0 69 651.0 70 534.5 71 418.0 72 418.0 73 418.0 74 336.5 75 255.0 76 255.0 77 255.0 78 177.5 79 100.0 80 100.0 81 100.0 82 64.0 83 28.0 84 28.0 85 28.0 86 18.5 87 9.0 88 9.0 89 9.0 90 4.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11424448319403524 2 0.12025735073056341 3 0.01202573507305634 4 0.0 5 0.018038602609584512 6 0.34874631711863385 7 0.030064337682640854 8 0.01202573507305634 9 0.01202573507305634 10 0.00601286753652817 11 0.01202573507305634 12 0.0 13 0.0 14 0.0 15 0.00601286753652817 16 0.0 17 0.0 18 0.0 19 0.00601286753652817 20 0.00601286753652817 21 0.018038602609584512 22 0.0 23 0.0 24 0.0 25 0.01202573507305634 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 16631.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.545427214238465 #Duplication Level Percentage of deduplicated Percentage of total 1 92.32555791174391 54.97564788647706 2 5.3822074118953855 6.40971679393903 3 0.9290114106836312 1.659551440081775 4 0.27264465313541353 0.6493896939450423 5 0.20195900232252853 0.601286753652817 6 0.060587700696758555 0.21646323131501413 7 0.0403918004645057 0.16836029102278877 8 0.07068565081288498 0.33672058204557753 9 0.030293850348379277 0.16234742348626058 >10 0.3938200545289306 5.796404305213156 >50 0.1514692517418964 6.548012747279178 >100 0.12117540139351711 16.090433527749383 >500 0.02019590023225285 6.385665323792916 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 539 3.240935602188684 No Hit GTACATGGGAAGCAGTGGTATCAAC 523 3.1447297216042327 No Hit GTATCAACGCAGAGTACTTTTTTTT 439 2.639648848535867 No Hit GGTATCAACGCAGAGTACTTTTTTT 367 2.2067223859058385 No Hit GAGTACATGGGAAGCAGTGGTATCA 264 1.5873970296434372 No Hit TATCAACGCAGAGTACTTTTTTTTT 261 1.5693584270338525 No Hit CATGTACTCTGCGTTGATACCACTG 209 1.2566893151343876 No Hit ACGCAGAGTACATGGGAAGCAGTGG 193 1.1604834345499369 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 192 1.1544705670134088 No Hit TATCAACGCAGAGTACATGGGAAGC 168 1.0101617461367327 No Hit GCGTTGATACCACTGCTTCCCATGT 163 0.9800974084540918 No Hit GTATCAACGCAGAGTACATGGGAAG 147 0.8838915278696411 No Hit GGTATCAACGCAGAGTACATGGGAA 143 0.8598400577235283 No Hit ACTCTGCGTTGATACCACTGCTTCC 130 0.7816727797486621 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 90 0.5411580782875353 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 85 0.5110937406048944 No Hit GTGGTATCAACGCAGAGTACATGGG 84 0.5050808730683664 No Hit GCTTCCCATGTACTCTGCGTTGATA 84 0.5050808730683664 No Hit CATGGGAAGCAGTGGTATCAACGCA 83 0.49906800553183817 No Hit ATACCACTGCTTCCCATGTACTCTG 80 0.48102940292225366 No Hit GTACTCTGCGTTGATACCACTGCTT 74 0.4449521977030846 No Hit GCAGTGGTATCAACGCAGAGTACAT 74 0.4449521977030846 No Hit ACATGGGAAGCAGTGGTATCAACGC 68 0.40887499248391557 No Hit GATACCACTGCTTCCCATGTACTCT 67 0.40286212494738743 No Hit CAGTGGTATCAACGCAGAGTACATG 65 0.39083638987433106 No Hit GGGAAGCAGTGGTATCAACGCAGAG 65 0.39083638987433106 No Hit CCACTGCTTCCCATGTACTCTGCGT 60 0.36077205219169023 No Hit GCAGAGTACATGGGAAGCAGTGGTA 57 0.3427334495821057 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 53 0.318681979435993 No Hit ATCAACGCAGAGTACTTTTTTTTTT 48 0.2886176417533522 No Hit GTTGATACCACTGCTTCCCATGTAC 48 0.2886176417533522 No Hit AAGCAGTGGTATCAACGCAGAGTAC 48 0.2886176417533522 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 47 0.282604774216824 No Hit CTGCTTCCCATGTACTCTGCGTTGA 47 0.282604774216824 No Hit GTGGTATCAACGCAGAGTACTTTTT 46 0.2765919066802959 No Hit TACCACTGCTTCCCATGTACTCTGC 46 0.2765919066802959 No Hit CTTCCCATGTACTCTGCGTTGATAC 46 0.2765919066802959 No Hit ATGGGAAGCAGTGGTATCAACGCAG 43 0.2585533040707113 No Hit CCATGTACTCTGCGTTGATACCACT 42 0.2525404365341832 No Hit ATCAACGCAGAGTACATGGGAAGCA 37 0.2224760988515423 No Hit AAAAAGTACTCTGCGTTGATACCAC 30 0.18038602609584511 No Hit TTCCCATGTACTCTGCGTTGATACC 30 0.18038602609584511 No Hit AACGCAGAGTACTTTTTTTTTTTTT 25 0.15032168841320426 No Hit CAACGCAGAGTACTTTTTTTTTTTT 24 0.1443088208766761 No Hit GTACATGGTAAGCAGTGGTATCAAC 22 0.13228308580361975 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 20 0.12025735073056341 No Hit GGAAGCAGTGGTATCAACGCAGAGT 20 0.12025735073056341 No Hit TCAACGCAGAGTACTTTTTTTTTTT 19 0.11424448319403524 No Hit TGGGAAGCAGTGGTATCAACGCAGA 18 0.10823161565750707 No Hit CCCCATGTACTCTGCGTTGATACCA 18 0.10823161565750707 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 40 2.4797634E-4 16.693941 4 TACATGG 275 9.094947E-12 8.32529 2 GAAGCAG 185 4.5486922E-7 8.200586 9 AGCAGTG 210 3.9863153E-8 8.127366 11 GCAGTGG 215 5.9459126E-8 7.9383583 12 GTACATG 290 3.274181E-11 7.8946705 1 TCAACGC 230 1.9041181E-8 7.880369 5 ATCAACG 230 1.9041181E-8 7.880369 4 ACATGGG 280 1.364242E-10 7.8359303 3 AACGCAG 230 2.1267624E-8 7.8328967 7 GGAAGCA 195 1.0128151E-6 7.780043 8 GGGAAGC 195 1.0128151E-6 7.780043 7 AGTACTT 135 5.482089E-4 7.726015 14 GAGTACT 135 5.482089E-4 7.726015 13 CAACGCA 235 2.7932401E-8 7.712702 6 AAGCAGT 210 3.6151505E-7 7.675846 10 AGTGGTA 215 5.266793E-7 7.4973383 14 TGGGAAG 205 1.9648578E-6 7.4453807 6 TGGTATC 220 7.598701E-7 7.3269444 16 GTGGTAT 220 7.598701E-7 7.3269444 15 >>END_MODULE