FastQCFastQC Report
Thu 26 May 2016
SRR1926788_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926788_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781871
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15900.20335835451116618No Hit
GTCCTAAAGTGTGTATTTCTCATTT12970.16588414201319657No Hit
CTGTAGGACGTGGAATATGGCAAGA12970.16588414201319657No Hit
GTCCTACAGTGTGCATTTCTCATTT10120.12943311620459128No Hit
CTTTAGGACGTGAAATATGGCGAGG8680.11101575579603284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1000.017.07374618
CCAACGA702.544948E-816.26070819
TAGGACC2900.013.7842334
TAGACAT853.877387E-612.3106825
GTAGGAC6700.012.0901983
GGCGAGG2750.012.07234519
ATAGGAC1209.5242285E-911.91243
AGGACCT4500.011.838155
TGTAGAA3850.011.6346772
TGTAGGA7300.011.6194172
CTGTAGG7000.011.585451
GTCCTAT1001.8281899E-611.4491511
GATATAC1850.011.34600451
GTTCTAG855.0867955E-511.2246571
TAGGACA2300.011.1729354
GTACTAA600.00568612711.1311191
GTCTTAG600.00568612711.1311191
TGGCGAG6600.011.06631618
GGACCTG4300.011.0613976
TATAGTG951.3375637E-511.0148215
Produced by FastQC (version 0.11.5)