##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926788_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 781871 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.31967038040802 31.0 28.0 31.0 22.0 34.0 2 28.334988764131168 31.0 28.0 31.0 20.0 34.0 3 27.877238061010065 31.0 28.0 31.0 16.0 34.0 4 31.76769185709663 35.0 32.0 37.0 19.0 37.0 5 31.562990570055675 35.0 32.0 37.0 19.0 37.0 6 31.165568233122855 35.0 32.0 37.0 17.0 37.0 7 31.027532674827434 35.0 32.0 37.0 17.0 37.0 8 31.227538813947568 35.0 32.0 37.0 17.0 37.0 9 33.40879889393519 37.0 33.0 39.0 18.0 39.0 10 33.574119004285876 37.0 34.0 39.0 19.0 39.0 11 33.749255311937645 38.0 34.0 39.0 23.0 39.0 12 33.6354245137625 38.0 34.0 39.0 21.0 39.0 13 33.725839684551545 38.0 34.0 39.0 23.0 39.0 14 34.76173691056453 39.0 34.0 41.0 23.0 41.0 15 34.365501470191376 39.0 34.0 41.0 17.0 41.0 16 34.43826667058888 39.0 34.0 41.0 18.0 41.0 17 34.65366921141723 39.0 34.0 41.0 22.0 41.0 18 34.702421499198714 39.0 35.0 41.0 22.0 41.0 19 34.04373483605352 39.0 34.0 41.0 11.0 41.0 20 34.368803805231295 39.0 34.0 41.0 18.0 41.0 21 34.376450846750934 39.0 34.0 41.0 18.0 41.0 22 34.39932034824159 39.0 34.0 41.0 18.0 41.0 23 34.38336247283759 39.0 34.0 41.0 16.0 41.0 24 33.02851109709914 39.0 32.0 40.0 2.0 41.0 25 33.65073010765203 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.048305524940609246 1101 2 -0.19234228181201019 1101 3 -1.24433901251043 1101 4 -0.5713966465802685 1101 5 -0.27564391199399196 1101 6 -2.3058528536133025 1101 7 -0.6618619043523815 1101 8 0.012404222792724795 1101 9 0.06495327940604767 1101 10 -9.063804132210144 1101 11 -3.8174240815094294 1101 12 -1.394585427053478 1101 13 -0.6925052373832798 1101 14 -0.47123968811100525 1101 15 -1.7423390652275899 1101 16 -0.20322791859970124 1101 17 0.1315932518607852 1101 18 0.16544023701506205 1101 19 -0.30696840582234586 1101 20 0.15041459120802614 1101 21 0.21616448883805361 1101 22 0.45685568493626505 1101 23 0.2602132193059603 1101 24 0.48975388481343174 1101 25 0.5324143539518502 1102 1 -0.1255324845205088 1102 2 -0.36811029581531685 1102 3 -0.6916599965961225 1102 4 -0.5267390210402887 1102 5 -0.3871103249309904 1102 6 -1.3233690328941883 1102 7 -0.5439037055118305 1102 8 0.12917206014879667 1102 9 -0.12151192911798603 1102 10 0.05207444690372398 1102 11 -0.24434431725319428 1102 12 -0.19339626998196735 1102 13 -0.32066682142721703 1102 14 -0.21755217707055152 1102 15 0.19606524817672266 1102 16 0.16914646112761744 1102 17 -0.08930411609540556 1102 18 -0.3206247049322286 1102 19 0.01755866135177797 1102 20 -0.14153511250638218 1102 21 -0.3106818762435992 1102 22 -0.3903665372859564 1102 23 -0.6570817494595929 1102 24 -4.4664837699536335 1102 25 -2.1944850053004465 1103 1 -0.28111619887905803 1103 2 -0.5929504279402167 1103 3 -0.23298767285757194 1103 4 -0.3472158042654172 1103 5 -0.7393877980693908 1103 6 0.007006948144042013 1103 7 -0.14739408632009088 1103 8 0.2417575535289913 1103 9 -0.05088124424889173 1103 10 0.1339894443816405 1103 11 -0.09593228390738773 1103 12 0.1405777552172296 1103 13 0.013685517064189412 1103 14 0.054433384650110384 1103 15 0.2589486463250239 1103 16 0.2724083624027642 1103 17 0.052274548662069265 1103 18 -0.08753015644033013 1103 19 -1.6240322070602815 1103 20 -0.48356151579105955 1103 21 -0.4028947242511549 1103 22 -0.26288309357734363 1103 23 -0.37093316860902803 1103 24 1.2783689997335728 1103 25 0.5683674769228944 1104 1 -0.6612679512904585 1104 2 -0.6063205167841588 1104 3 -1.50817960621918 1104 4 -1.0508269244385389 1104 5 -1.1592243556657102 1104 6 -1.739986277097639 1104 7 -1.0074662380398784 1104 8 -0.14839457948680845 1104 9 -0.4172700697037115 1104 10 -0.28221505979956873 1104 11 -0.42213608125185687 1104 12 -0.3302964930890866 1104 13 -0.5660443081840185 1104 14 -0.6670484713944305 1104 15 -0.4101688408159845 1104 16 -0.783409991428833 1104 17 -0.7850170704290491 1104 18 -0.8969329881370669 1104 19 -1.6809461578426976 1104 20 -1.0252349789648676 1104 21 -0.7366752677542365 1104 22 -0.6489872269411308 1104 23 -0.5632960694670217 1104 24 0.6793003930719053 1104 25 0.11690677160856922 1105 1 0.031170333199256106 1105 2 0.027241031739400512 1105 3 0.3463749566930687 1105 4 -0.03195742456377815 1105 5 -0.14852328973378803 1105 6 -0.21873721639796528 1105 7 0.022452216776294165 1105 8 0.3587580185483148 1105 9 -0.4335373597874863 1105 10 0.087577041728359 1105 11 -0.209394994168278 1105 12 -0.07114701213948393 1105 13 -0.14696329179148648 1105 14 -0.2708863829471255 1105 15 0.26334428155745115 1105 16 -0.4980992569655598 1105 17 -0.3739931634963156 1105 18 -0.4322785115012948 1105 19 -0.9778624696625755 1105 20 -0.2593086201103958 1105 21 -0.24598477764819648 1105 22 -0.07156104898894 1105 23 -0.3845924430719805 1105 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2215 2 0.06051719531217259 2215 3 0.4001780535752637 2215 4 0.514523832722567 2215 5 0.8527602274395605 2215 6 1.273077792719306 2215 7 0.9333178342097099 2215 8 -0.9125685440918083 2215 9 -0.26901549328821517 2215 10 -0.23718830069235253 2215 11 -0.37030171404392576 2215 12 -0.5594365526889149 2215 13 -0.11870349446389383 2215 14 -0.13872334824171872 2215 15 -0.290950176338697 2215 16 -0.13151404786622578 2215 17 -0.7154928047840912 2215 18 -1.4212518472260385 2215 19 0.025638711098494582 2215 20 -3.6140189599902293 2215 21 -1.9484143056427001 2215 22 -1.209039376792127 2215 23 -1.0141604190382623 2215 24 -0.9033107484472822 2215 25 -0.5462439612260681 2216 1 -1.0319120823176569 2216 2 -1.295390604966407 2216 3 -0.4666014147390811 2216 4 -0.8126240118433117 2216 5 -0.6487688450273126 2216 6 -1.9607601717318097 2216 7 -10.193188916031371 2216 8 -10.790151225616146 2216 9 -6.176461592553665 2216 10 -3.944482906452084 2216 11 -3.0448161312254918 2216 12 -2.6774794589831856 2216 13 -2.3975036301830244 2216 14 -2.710484839723563 2216 15 -3.666465819754535 2216 16 -2.922892013745958 2216 17 -3.1669709825761565 2216 18 -2.8540872461093336 2216 19 -2.1371543053187594 2216 20 -3.128916813484995 2216 21 -5.762379387728963 2216 22 -4.6507329789445855 2216 23 -4.270551272283022 2216 24 -4.811716703421862 2216 25 -3.93295048050652 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41783.0 3 7.0 4 283.0 5 1823.0 6 2190.0 7 1835.0 8 1340.0 9 1321.0 10 1318.0 11 1330.0 12 1290.0 13 1281.0 14 1314.0 15 1379.0 16 1506.0 17 1642.0 18 1855.0 19 2398.0 20 2970.0 21 3953.0 22 4869.0 23 5938.0 24 7046.0 25 8019.0 26 9190.0 27 10099.0 28 11486.0 29 13623.0 30 16336.0 31 20124.0 32 25910.0 33 34386.0 34 46916.0 35 65623.0 36 96155.0 37 151565.0 38 181768.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29103086943483 18.520507424505354 12.40954868542808 25.778913020631745 2 14.385891223505066 20.20709331748845 41.872368434532284 23.534647024474197 3 19.573382576480334 24.073521521039538 29.053883596474268 27.29921230600586 4 11.654844580740452 17.010876660360985 38.682283253936355 32.65199550496221 5 12.59497112008034 37.970665933208394 36.19910833647825 13.23525461023302 6 31.37492196375024 38.21102537073615 18.15941910327394 12.254633562239665 7 27.67230129041687 31.191014001522966 22.292701311953728 18.84398339610644 8 24.288543502643808 37.68383271911553 21.40003204614645 16.627591732094217 9 25.96309936665383 15.591496198771685 20.121756995142768 38.32364743943171 10 13.812987378535194 29.71744039508447 35.431196064508285 21.038376161872048 11 34.397577723653775 22.681977731840604 24.474508278925068 18.44593626558055 12 22.84973437086254 24.81883107828928 32.40649892737765 19.92493562347054 13 28.76058366709434 22.196311594416095 25.765752858661433 23.277351879828128 14 20.091514622000094 22.443340629089473 27.936520525550286 29.52862422336015 15 22.761321947001367 31.79150527786164 23.088534018287035 22.358638756849963 16 21.56473706563509 28.673968551238556 28.45944051292285 21.3018538702035 17 21.33936281125474 29.000978617526847 28.178684047255075 21.480974523963337 18 20.9998554530651 28.262123714267805 30.007687850254495 20.7303329824126 19 23.471783174423262 27.207715699155276 27.21564734373061 22.104853782690846 20 22.82224237687292 28.07889650302597 28.388337194412927 20.710523925688182 21 22.49586508339548 27.312579228583818 27.18261556979982 23.00894011822088 22 22.23637873095427 28.86392738222702 27.62194958150887 21.27774430530984 23 21.82893195040705 28.295792376849114 27.768112530765332 22.107163141978496 24 23.01880903406707 27.433764160501028 28.32112997472344 21.22629683070846 25 22.258891852306668 27.830074639752866 28.198604422101553 21.712429085838913 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 212.0 1 212.0 2 271.5 3 331.0 4 331.0 5 331.0 6 457.5 7 584.0 8 584.0 9 584.0 10 954.0 11 1324.0 12 1324.0 13 1324.0 14 2378.0 15 3432.0 16 3432.0 17 3432.0 18 6087.5 19 8743.0 20 8743.0 21 8743.0 22 14009.0 23 19275.0 24 19275.0 25 19275.0 26 28230.5 27 37186.0 28 37186.0 29 37186.0 30 49003.5 31 60821.0 32 60821.0 33 60821.0 34 69762.0 35 78703.0 36 78703.0 37 78703.0 38 85455.5 39 92208.0 40 92208.0 41 92208.0 42 96680.0 43 101152.0 44 101152.0 45 101152.0 46 102899.5 47 104647.0 48 104647.0 49 104647.0 50 99821.5 51 94996.0 52 94996.0 53 94996.0 54 83232.5 55 71469.0 56 71469.0 57 71469.0 58 60362.5 59 49256.0 60 49256.0 61 49256.0 62 39771.5 63 30287.0 64 30287.0 65 30287.0 66 23143.0 67 15999.0 68 15999.0 69 15999.0 70 11563.5 71 7128.0 72 7128.0 73 7128.0 74 4922.0 75 2716.0 76 2716.0 77 2716.0 78 1856.0 79 996.0 80 996.0 81 996.0 82 640.0 83 284.0 84 284.0 85 284.0 86 185.5 87 87.0 88 87.0 89 87.0 90 57.0 91 27.0 92 27.0 93 27.0 94 16.0 95 5.0 96 5.0 97 5.0 98 4.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20681160958777087 2 0.12367769107691677 3 0.12585196279181604 4 0.07226255993635779 5 0.023789090527721327 6 0.02340539551920969 7 0.23187968347719765 8 0.22331049495377114 9 0.019184750425581713 10 0.034020957421364906 11 0.016243088693659185 12 0.018161563736217357 13 0.01585939368514755 14 0.017649970391535177 15 0.014708308659612649 16 0.019824242106434437 17 0.01995214044260498 18 0.015092003668124281 19 0.024556480544744592 20 0.017649970391535177 21 0.014580410323442101 22 0.017138377046852997 23 0.01880105541707008 24 0.015219902004294825 25 0.014836206995783193 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 781871.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.39188461014739 #Duplication Level Percentage of deduplicated Percentage of total 1 93.61588334593904 80.87652591699384 2 4.865280050886544 8.406414255044844 3 0.7817993617326966 2.02623360741294 4 0.2734145524424946 0.9448319386138833 5 0.12146470537440894 0.5246782405455743 6 0.07713932384841843 0.3998526938890413 7 0.043225342759769254 0.26140231767552385 8 0.03868068853871798 0.2673358064702379 9 0.02618780143678813 0.2036172167928407 >10 0.1314695582191268 2.1717856177019117 >50 0.013616465851693299 0.8234235216610754 >100 0.010506937666328237 2.0017014368252215 >500 7.399251689064704E-4 0.4279041085636139 >1k 5.919401351251762E-4 0.6642933218095421 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1590 0.20335835451116618 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1297 0.16588414201319657 No Hit CTGTAGGACGTGGAATATGGCAAGA 1297 0.16588414201319657 No Hit GTCCTACAGTGTGCATTTCTCATTT 1012 0.12943311620459128 No Hit CTTTAGGACGTGAAATATGGCGAGG 868 0.11101575579603284 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.2789833617054476E-4 0.0 0.0 0.0 6 0.0 1.2789833617054476E-4 0.0 0.0 0.0 7 0.0 1.2789833617054476E-4 0.0 0.0 0.0 8 0.0 1.2789833617054476E-4 0.0 0.0 0.0 9 0.0 1.2789833617054476E-4 0.0 0.0 0.0 10 0.0 1.2789833617054476E-4 0.0 0.0 0.0 11 0.0 1.2789833617054476E-4 0.0 0.0 0.0 12 0.0 1.2789833617054476E-4 0.0 0.0 0.0 13 0.0 1.2789833617054476E-4 0.0 0.0 1.2789833617054476E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 100 0.0 17.073746 18 CCAACGA 70 2.544948E-8 16.260708 19 TAGGACC 290 0.0 13.784233 4 TAGACAT 85 3.877387E-6 12.310682 5 GTAGGAC 670 0.0 12.090198 3 GGCGAGG 275 0.0 12.072345 19 ATAGGAC 120 9.5242285E-9 11.9124 3 AGGACCT 450 0.0 11.83815 5 TGTAGAA 385 0.0 11.634677 2 TGTAGGA 730 0.0 11.619417 2 CTGTAGG 700 0.0 11.58545 1 GTCCTAT 100 1.8281899E-6 11.449151 1 GATATAC 185 0.0 11.3460045 1 GTTCTAG 85 5.0867955E-5 11.224657 1 TAGGACA 230 0.0 11.172935 4 GTACTAA 60 0.005686127 11.131119 1 GTCTTAG 60 0.005686127 11.131119 1 TGGCGAG 660 0.0 11.066316 18 GGACCTG 430 0.0 11.061397 6 TATAGTG 95 1.3375637E-5 11.014821 5 >>END_MODULE