FastQCFastQC Report
Thu 26 May 2016
SRR1926788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926788_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781871
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16190.20706740626011197No Hit
CTGTAGGACGTGGAATATGGCAAGA15870.20297465950265453No Hit
GTATCAACGCAGAGTACTTTTTTTT13010.16639573535787874No Hit
GTCCTAAAGTGTGTATTTCTCATTT11590.14823417162166136No Hit
CTTTAGGACGTGAAATATGGCGAGG11320.14478091654505668No Hit
GTCCTACAGTGTGCATTTCTCATTT9860.12610775946415712No Hit
GGTATCAACGCAGAGTACTTTTTTT8090.1034697539619707No Hit
CTGTAGGACCTGGAATATGGCGAGA8080.10334185562580016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTCC400.005318729714.2326838
TCCAACG954.974936E-913.98209418
TAGGACC3000.013.96552854
CCTAGAC655.3226082E-513.1859333
CTAGGAC1102.6393536E-912.9861463
GTCCTAA3450.012.7122541
ATTATAC1051.9210347E-812.6975653
TAATCCT604.0091338E-412.6959354
TAGGACT1051.9244908E-812.6959344
CCAACGA751.4959127E-512.65046519
GTCCTAG1251.3278623E-912.2037641
CTAGACT657.8618363E-411.7193244
GATATAC3000.011.4410291
TGTAGGA8950.011.290442
TCCTAGA1552.928573E-1011.070542
TGACGAA600.005927430511.06915718
AGGACCT6050.011.0171345
GTCCTAC7900.010.9824211
CTGTAGG9000.010.8054161
GTCCAGG1502.0008883E-910.8054161
Produced by FastQC (version 0.11.5)