FastQCFastQC Report
Thu 26 May 2016
SRR1926787_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926787_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624463
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18780.30073839442849293No Hit
GTCCTAAAGTGTGTATTTCTCATTT15730.2518964294121509No Hit
CTGTAGGACGTGGAATATGGCAAGA14540.23284005617626663No Hit
CTTTAGGACGTGAAATATGGCGAGG12900.20657749138059422No Hit
GTCCTACAGTGTGCATTTCTCATTT12350.1977699239186309No Hit
GTATCAACGCAGAGTACTTTTTTTT11720.18768125573492744No Hit
GTACATGGGAAGCAGTGGTATCAAC9350.14972864685337642No Hit
CCCATGTACTCTGCGTTGATACCAC8640.13835887794793286No Hit
CTGTAGGACCTGGAATATGGCGAGA7930.1269891090424893No Hit
CTGAAGGACCTGGAATATGGCGAGA7450.1193025047120486No Hit
GGTATCAACGCAGAGTACTTTTTTT6670.10681177267508243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTC500.001520688213.27325213
TAGGACC3450.012.4191124
ATAGGAC701.04882376E-412.2634013
ACTGTTC2100.012.2269418
TAGTACC550.003013857412.1180434
CCAGTAC657.7462546E-411.7393253
CAGGACT1406.511982E-1011.5616024
TAGAAAT2500.011.4255834
GTACCAT855.212146E-511.1970486
CTATAAC600.00566888911.135081
CTTATAC600.00569618311.1279013
GCAGGAC700.001440178310.9008023
CAGTACC700.001461630210.8815084
AAAGGCG700.001466544310.8771325
AGTACCA700.001466544310.8771325
ATTTAGA2300.010.789271
AGAAATG3000.010.78648955
TGTAGGA9400.010.6578092
TAGGACA2250.010.5792444
CAGTGCA909.6575284E-510.53770359