FastQCFastQC Report
Thu 26 May 2016
SRR1926787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926787_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624463
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20130.3223569691078575No Hit
GTCCTACAGTGGACATTTCTAAATT18050.2890483503426144No Hit
CTGTAGGACGTGGAATATGGCAAGA17540.2808813332415211No Hit
CTTTAGGACGTGAAATATGGCGAGG15950.25541945639693625No Hit
GTCCTAAAGTGTGTATTTCTCATTT15590.2496545031491057No Hit
GGTATCAACGCAGAGTACTTTTTTT12510.20033212536211112No Hit
GTCCTACAGTGTGCATTTCTCATTT12270.1964888231968908No Hit
TATCAACGCAGAGTACTTTTTTTTT10950.1753506612881788No Hit
GTACATGGGAAGCAGTGGTATCAAC10380.16622281864578045No Hit
CCCATGTACTCTGCGTTGATACCAC9960.15949703985664482No Hit
CTGTAGGACCTGGAATATGGCGAGA9460.1514901603457691No Hit
CTGAAGGACCTGGAATATGGCGAGA8580.13739805240662778No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6730.10777259821638753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGA350.002188064916.26354217
TGTCGAG350.002188064916.26354218
TAAGAGT852.6124872E-713.4441084
GTATAGT751.4143297E-512.7186841
GCCGGTT751.4972706E-512.64840911
CCGGTTT751.498504E-512.64739612
GTCCTAT853.756426E-612.3446051
TCTAGGG550.002974023312.1395852
CCAGTAC550.003006470612.1220163
CTAATAC550.003006470612.1220163
TATGACC550.003010094612.1200674
CCAACGT550.00309634212.07444819
CTGTACT1052.626184E-711.790274
TAAGGAG657.851292E-411.7205045
GTCCTAA5400.011.6587931
CTATACT1156.810842E-811.5931084
GGCGAGG3950.011.52858619
TGTCCAC2400.011.46262110
CTAGGAC855.1735355E-511.2052253
CGGTTTC855.3938078E-511.15946813