##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926787_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 624463 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.975031346933285 31.0 30.0 33.0 26.0 34.0 2 30.260441371226158 31.0 30.0 34.0 26.0 34.0 3 30.306328797702985 31.0 30.0 34.0 26.0 34.0 4 33.92850497147149 37.0 35.0 37.0 30.0 37.0 5 33.92903822964691 37.0 35.0 37.0 30.0 37.0 6 33.89268379391573 37.0 35.0 37.0 30.0 37.0 7 33.86732760788069 37.0 35.0 37.0 30.0 37.0 8 33.90682234175603 37.0 35.0 37.0 30.0 37.0 9 35.42003769638874 39.0 35.0 39.0 30.0 39.0 10 35.310934354797645 39.0 35.0 39.0 30.0 39.0 11 35.372566509144654 39.0 35.0 39.0 30.0 39.0 12 35.275142002008124 39.0 35.0 39.0 30.0 39.0 13 35.287608713406556 39.0 35.0 39.0 30.0 39.0 14 36.375195968376026 40.0 37.0 41.0 30.0 41.0 15 36.376326539762964 40.0 37.0 41.0 30.0 41.0 16 36.36353635043229 40.0 37.0 41.0 30.0 41.0 17 36.35195039578005 40.0 37.0 41.0 30.0 41.0 18 36.33583895282827 40.0 37.0 41.0 30.0 41.0 19 36.39921020140505 40.0 37.0 41.0 29.0 41.0 20 36.37283554029622 40.0 37.0 41.0 29.0 41.0 21 36.32234415810064 40.0 37.0 41.0 29.0 41.0 22 36.244418003948994 40.0 37.0 41.0 29.0 41.0 23 36.16866171414479 40.0 37.0 41.0 29.0 41.0 24 36.11764027652559 40.0 37.0 41.0 29.0 41.0 25 36.00144283968786 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.007195257672602651 1101 2 0.1572746594926322 1101 3 0.29971125470395066 1101 4 0.26804142088798244 1101 5 0.15152341112621315 1101 6 0.3046817527744281 1101 7 0.20858691356409764 1101 8 0.03419015379384405 1101 9 0.11214350243434268 1101 10 0.3488775709904175 1101 11 0.2085465351835012 1101 12 0.2905675256598812 1101 13 0.14371720537463517 1101 14 0.303301993520364 1101 15 0.16699287064953694 1101 16 0.067778552523869 1101 17 0.08518572500911148 1101 18 0.04317789989150356 1101 19 0.16518798691961933 1101 20 0.35732259792178667 1101 21 0.3939034943055191 1101 22 0.23665438276743345 1101 23 0.42595130802138925 1101 24 0.20497825428348904 1101 25 0.2781063221707214 1102 1 -0.9865062255340931 1102 2 -0.9495098771786026 1102 3 -0.598870873959708 1102 4 -0.6456030936444392 1102 5 -0.7684913010809282 1102 6 -0.5797228376222137 1102 7 -0.6137749362256741 1102 8 -0.8065525541453411 1102 9 -0.7958840512264302 1102 10 -0.6082831140437932 1102 11 -0.6086390148908833 1102 12 -0.6289935993304283 1102 13 -0.5386518830090665 1102 14 -0.7865047501229512 1102 15 -0.7387843733733419 1102 16 -0.7931934402281371 1102 17 -0.6548300682867279 1102 18 -0.7103562025816004 1102 19 -0.7600770560771579 1102 20 -0.8518833424256158 1102 21 -0.9100445946351741 1102 22 -0.8383766985327838 1102 23 -1.010656940685422 1102 24 -0.9636633577607796 1102 25 -0.8003842429801509 1103 1 -0.90545509236836 1103 2 -0.7358488044233589 1103 3 -0.5022293314284703 1103 4 -0.5631964766319442 1103 5 -0.9790204557092679 1103 6 -0.9316889927383443 1103 7 -0.8345933389122564 1103 8 -0.9117327068600645 1103 9 -0.9870014108001826 1103 10 -0.9757435282871612 1103 11 -0.9392387109623286 1103 12 -0.6793903949478519 1103 13 -0.7407972058862597 1103 14 -0.8424700516125796 1103 15 -0.787776437460991 1103 16 -0.9039784482953763 1103 17 -0.8424391167276895 1103 18 -0.6580235039961835 1103 19 -0.8671954568876359 1103 20 -0.7206453025772035 1103 21 -0.9426641492485146 1103 22 -0.8213956284040833 1103 23 -1.0946019615101577 1103 24 -1.1609448856584237 1103 25 -0.9239771663226151 1104 1 0.09781419459832819 1104 2 -0.13230920769629861 1104 3 0.01707589943067589 1104 4 -0.03439387581370568 1104 5 0.0865844537004321 1104 6 -0.03130179227078145 1104 7 0.1379921374173776 1104 8 0.05712687817792528 1104 9 0.11737833788479435 1104 10 0.024475641788818336 1104 11 0.15420079809670284 1104 12 0.2661093128253782 1104 13 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0.12638882944701635 2214 11 0.3375155749382017 2214 12 0.29814529372954723 2214 13 0.18177140111458812 2214 14 0.3673414828375883 2214 15 0.11150082608115497 2214 16 0.17914642948312576 2214 17 0.3269945088508308 2214 18 0.29158660942536585 2214 19 0.153447754115561 2214 20 0.1027120404903954 2214 21 0.2945698152909557 2214 22 0.4063641330195509 2214 23 0.3306331111708971 2214 24 0.40624434581280155 2214 25 0.5472844854601888 2215 1 0.5862700479684513 2215 2 0.6856618210778436 2215 3 0.5523025450751398 2215 4 0.5359825530392683 2215 5 0.6828901296957 2215 6 0.5267953697315804 2215 7 0.21574046221044085 2215 8 0.4729595545673604 2215 9 0.35402004068338044 2215 10 0.5414316922421136 2215 11 0.301308602590197 2215 12 0.2649760846030702 2215 13 0.29089084543691257 2215 14 0.4213521216031424 2215 15 0.541475767831713 2215 16 0.577646451464048 2215 17 0.27749171727391797 2215 18 0.2913078913526519 2215 19 0.18810726965607216 2215 20 0.35928653383017206 2215 21 0.3674471177923877 2215 22 0.23772826894956722 2215 23 0.19993191980500313 2215 24 0.3343157398427792 2215 25 0.37322812637987113 2216 1 -0.13794029566223287 2216 2 0.06515158157766976 2216 3 -1.7195639943209038 2216 4 -1.449663923878468 2216 5 -0.30889050202939217 2216 6 -0.11388147858497177 2216 7 -0.3111920265087562 2216 8 -0.15853211499182152 2216 9 -0.3920290742072865 2216 10 -0.43016324007305684 2216 11 -0.3451509600616589 2216 12 -0.5143106214017905 2216 13 -0.6189173808733912 2216 14 -0.5951881421941536 2216 15 -0.6948466896483296 2216 16 -0.49071609626243173 2216 17 -0.39430800504472074 2216 18 -0.27229589205588667 2216 19 -0.5174463575567003 2216 20 -0.42799218883556733 2216 21 -0.35743833864634666 2216 22 -0.4690731888775801 2216 23 -0.7180418740165848 2216 24 -0.821231813090229 2216 25 -0.5380726372438716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14935.0 3 16.0 4 240.0 5 1531.0 6 2124.0 7 1606.0 8 1243.0 9 1020.0 10 915.0 11 808.0 12 763.0 13 742.0 14 777.0 15 756.0 16 727.0 17 751.0 18 849.0 19 1075.0 20 1421.0 21 1854.0 22 2318.0 23 2809.0 24 3327.0 25 3890.0 26 4730.0 27 5106.0 28 5957.0 29 7278.0 30 9387.0 31 11986.0 32 15818.0 33 20960.0 34 28285.0 35 40411.0 36 59974.0 37 106072.0 38 262002.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.24212170137581 20.100944834673975 10.687390072582158 27.969543391368063 2 13.397096197477811 21.863340094441728 41.387764264468906 23.351799443611554 3 18.058943463797387 26.26012835523039 29.820888876787627 25.8600393041846 4 11.543197915649126 18.323583623048922 39.10143595377148 31.03178250753047 5 11.923968207449118 39.70497563350485 36.02525851866443 12.345797640381601 6 30.007231820008034 39.27987143431097 18.476014463640016 12.23688228204098 7 26.768892669104588 32.65593790194825 22.79229797069692 17.78287145825024 8 23.85078226722165 39.51086077421152 20.64035566773382 15.998001290833002 9 26.384882840088853 15.683300688817958 20.418223487074854 37.51359298401834 10 14.39507011126841 30.422493257915946 34.93984972038768 20.24258691042797 11 33.25779789920744 23.105355115296966 25.654075470308168 17.982771515187427 12 23.147076109080018 25.68287109321829 32.65738823525644 18.512664562445252 13 28.876583165321772 23.30329676312852 26.005306976736737 21.814813094812965 14 19.839766327228357 23.948576617029353 28.20551417778155 28.006142877960745 15 21.757866922892145 33.3573544719353 23.51477300024021 21.37000560493234 16 20.336388254115175 29.285283053917244 29.905822178308515 20.472506513659066 17 20.377828630359204 30.276573632064668 28.895226778848386 20.45037095872774 18 20.221471351247477 28.37683758767575 31.876341158761168 19.5253499023156 19 22.76738745057722 27.538717513880012 28.282561385134976 21.411333650407794 20 22.72438294390166 28.680917981542365 29.096639160102296 19.498059914453677 21 21.48047542551096 27.705179806107626 28.088928466751124 22.72541630163029 22 22.169127714532326 29.88055337145676 27.82646850173669 20.123850412274226 23 20.720396116977494 29.589310478459858 28.90488132184184 20.785412082720807 24 22.37394369242053 27.834795656428003 29.707284498841403 20.083976152310065 25 21.394265032070017 28.63256007623135 29.199971173017946 20.773203718680687 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 255.0 1 255.0 2 251.5 3 248.0 4 248.0 5 248.0 6 515.5 7 783.0 8 783.0 9 783.0 10 1142.0 11 1501.0 12 1501.0 13 1501.0 14 2419.5 15 3338.0 16 3338.0 17 3338.0 18 5972.5 19 8607.0 20 8607.0 21 8607.0 22 13828.5 23 19050.0 24 19050.0 25 19050.0 26 27499.5 27 35949.0 28 35949.0 29 35949.0 30 46981.5 31 58014.0 32 58014.0 33 58014.0 34 64490.0 35 70966.0 36 70966.0 37 70966.0 38 74072.0 39 77178.0 40 77178.0 41 77178.0 42 78430.5 43 79683.0 44 79683.0 45 79683.0 46 80611.5 47 81540.0 48 81540.0 49 81540.0 50 75503.5 51 69467.0 52 69467.0 53 69467.0 54 58898.0 55 48329.0 56 48329.0 57 48329.0 58 40405.5 59 32482.0 60 32482.0 61 32482.0 62 25938.5 63 19395.0 64 19395.0 65 19395.0 66 14826.0 67 10257.0 68 10257.0 69 10257.0 70 7399.5 71 4542.0 72 4542.0 73 4542.0 74 3233.0 75 1924.0 76 1924.0 77 1924.0 78 1296.0 79 668.0 80 668.0 81 668.0 82 444.0 83 220.0 84 220.0 85 220.0 86 138.5 87 57.0 88 57.0 89 57.0 90 32.5 91 8.0 92 8.0 93 8.0 94 4.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12106401820444125 2 0.1277897969935769 3 0.01633403420218652 4 0.0 5 0.006405503608700595 6 0.35438448715136045 7 0.02562201443480238 8 0.009608255413050893 9 0.009608255413050893 10 0.004323714935872902 11 0.0051244028869604765 12 6.405503608700596E-4 13 3.202751804350298E-4 14 0.0 15 0.0020817886728276935 16 9.608255413050893E-4 17 0.0 18 4.8041277065254465E-4 19 6.405503608700596E-4 20 9.608255413050893E-4 21 0.015373208660881429 22 0.0 23 1.601375902175149E-4 24 0.0 25 0.007686604330440714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 624463.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.2486872906214 #Duplication Level Percentage of deduplicated Percentage of total 1 90.70552630437936 71.88293889626993 2 7.073801844879239 11.211790207213111 3 1.1103852207985776 2.639897134055822 4 0.40781316133947304 1.2927463078396653 5 0.18302213824484964 0.7252132100513484 6 0.10891248143227203 0.517870270984304 7 0.0692618147159231 0.3842235526922205 8 0.047739406886254256 0.3026628262214801 9 0.03721280482508937 0.2654159339511391 >10 0.20995732407256873 3.1120211516099685 >50 0.023487527222818237 1.3366443127517704 >100 0.019640329263531695 3.3746522816525197 >500 0.0014173433487851585 0.8138574662063438 >1k 0.001822298591295204 2.1400664485003955 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2013 0.3223569691078575 No Hit GTCCTACAGTGGACATTTCTAAATT 1805 0.2890483503426144 No Hit CTGTAGGACGTGGAATATGGCAAGA 1754 0.2808813332415211 No Hit CTTTAGGACGTGAAATATGGCGAGG 1595 0.25541945639693625 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1559 0.2496545031491057 No Hit GGTATCAACGCAGAGTACTTTTTTT 1251 0.20033212536211112 No Hit GTCCTACAGTGTGCATTTCTCATTT 1227 0.1964888231968908 No Hit TATCAACGCAGAGTACTTTTTTTTT 1095 0.1753506612881788 No Hit GTACATGGGAAGCAGTGGTATCAAC 1038 0.16622281864578045 No Hit CCCATGTACTCTGCGTTGATACCAC 996 0.15949703985664482 No Hit CTGTAGGACCTGGAATATGGCGAGA 946 0.1514901603457691 No Hit CTGAAGGACCTGGAATATGGCGAGA 858 0.13739805240662778 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 673 0.10777259821638753 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTCGA 35 0.0021880649 16.263542 17 TGTCGAG 35 0.0021880649 16.263542 18 TAAGAGT 85 2.6124872E-7 13.444108 4 GTATAGT 75 1.4143297E-5 12.718684 1 GCCGGTT 75 1.4972706E-5 12.648409 11 CCGGTTT 75 1.498504E-5 12.647396 12 GTCCTAT 85 3.756426E-6 12.344605 1 TCTAGGG 55 0.0029740233 12.139585 2 CCAGTAC 55 0.0030064706 12.122016 3 CTAATAC 55 0.0030064706 12.122016 3 TATGACC 55 0.0030100946 12.120067 4 CCAACGT 55 0.003096342 12.074448 19 CTGTACT 105 2.626184E-7 11.79027 4 TAAGGAG 65 7.851292E-4 11.720504 5 GTCCTAA 540 0.0 11.658793 1 CTATACT 115 6.810842E-8 11.593108 4 GGCGAGG 395 0.0 11.528586 19 TGTCCAC 240 0.0 11.462621 10 CTAGGAC 85 5.1735355E-5 11.205225 3 CGGTTTC 85 5.3938078E-5 11.159468 13 >>END_MODULE