FastQCFastQC Report
Thu 26 May 2016
SRR1926786_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926786_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474332
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT7230.15242488383663763No Hit
GTCCTACAGTGGACATTTCTAAATT5440.11468760277611463No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATAAG400.005088840614.3260921
TAGGAGT400.00517922314.2881444
GTGTTAT551.8691986E-413.8919691
TCTAAAC706.9250564E-613.6294173
CTAGCCA500.001466997813.3356014
TTAGACT655.3021395E-513.1890554
CTTAGAC603.9383795E-412.720793
GTGTAGC853.6998354E-612.3597651
GTCCTAC2900.012.1854111
CTAGACA550.003002287412.1232734
CTAAACA802.779375E-511.9067874
GTCTATG657.655373E-411.7547431
ACTGGAC657.7239156E-411.7422683
TAGCACT657.8277336E-411.7236054
CAGTCCT752.0172725E-411.4305144
TTGGACA855.161945E-511.2063874
GTGCAAG600.00563735911.1425161
GGATAGG600.00563735911.1425161
TCTGCGT600.00594777411.06259810
AATCCCG600.005957120611.06026619
Produced by FastQC (version 0.11.5)