##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926786_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 474332 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.674430567619304 30.0 27.0 31.0 10.0 31.0 2 27.27268453319616 31.0 28.0 31.0 10.0 34.0 3 26.896205611259624 31.0 27.0 31.0 10.0 34.0 4 30.601234999957835 35.0 31.0 37.0 10.0 37.0 5 30.192917618882976 35.0 30.0 37.0 10.0 37.0 6 29.9955621800764 35.0 30.0 37.0 10.0 37.0 7 30.058984002765996 35.0 30.0 37.0 10.0 37.0 8 29.750963460192438 35.0 30.0 37.0 8.0 37.0 9 31.93635048868725 37.0 32.0 39.0 10.0 39.0 10 32.095129571692404 37.0 32.0 39.0 8.0 39.0 11 32.29344003777945 37.0 32.0 39.0 8.0 39.0 12 32.14133138814164 37.0 32.0 39.0 2.0 39.0 13 32.27387146555577 37.0 32.0 39.0 2.0 39.0 14 33.216295759088574 38.0 33.0 40.0 2.0 41.0 15 32.765040098496414 38.0 32.0 40.0 2.0 41.0 16 32.855970080028335 38.0 32.0 40.0 2.0 41.0 17 33.08756946611234 38.0 33.0 40.0 2.0 41.0 18 33.13986827791505 38.0 33.0 40.0 2.0 41.0 19 32.49767040806861 38.0 32.0 40.0 2.0 41.0 20 32.769882698194515 39.0 32.0 40.0 2.0 41.0 21 32.757701356855534 39.0 32.0 40.0 2.0 41.0 22 32.77831982661933 39.0 32.0 40.0 2.0 41.0 23 32.76353693193797 39.0 33.0 40.0 2.0 41.0 24 31.44363652462832 38.0 30.0 40.0 2.0 41.0 25 32.017312768272014 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.837759630192533 1101 2 -0.6846218167450253 1101 3 -1.7928721685468787 1101 4 -1.09219040646785 1101 5 -1.096809205912269 1101 6 -3.455510195784168 1101 7 -1.4042919026320284 1101 8 -0.9412894532087037 1101 9 -0.882812623540083 1101 10 -9.421363430823089 1101 11 -4.899054933247552 1101 12 -2.5624241949870026 1101 13 -1.705256672926609 1101 14 -1.2766344142712178 1101 15 -2.9412059253532945 1101 16 -1.0878736300441112 1101 17 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1.9179685396284114 2215 8 0.5238983319790833 2215 9 1.2185246877972276 2215 10 1.295505646045985 2215 11 1.0657572815646645 2215 12 0.7089868264240167 2215 13 1.157807560137627 2215 14 1.3171107594739553 2215 15 1.3653051311577613 2215 16 1.6133022211317396 2215 17 1.0457210734802302 2215 18 0.5019646954901944 2215 19 1.5026386321591687 2215 20 -2.3540581187217455 2215 21 -0.4966586121878862 2215 22 0.2215317621454318 2215 23 0.664884438352118 2215 24 0.4648110846548299 2215 25 0.6613091943984308 2216 1 0.9768363294034259 2216 2 -0.036057603180811526 2216 3 0.7602952196205095 2216 4 0.5601833309058861 2216 5 0.8117550076519429 2216 6 -0.3441393444133176 2216 7 -7.956110084450213 2216 8 -9.389355831275079 2216 9 -4.498476404203863 2216 10 -2.169891569351229 2216 11 -1.2065946157872354 2216 12 -0.7483910059538132 2216 13 -0.4895784622483994 2216 14 -0.4428537504905563 2216 15 -1.3540847132320835 2216 16 -0.4961708883413678 2216 17 -0.7528388500796979 2216 18 -0.3488747803492771 2216 19 0.20588733540787274 2216 20 -0.6160701307337533 2216 21 -3.069242559771837 2216 22 -1.558259392645727 2216 23 -1.3436126951450191 2216 24 -1.5742962044524589 2216 25 -1.1179700848808487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38214.0 3 9.0 4 297.0 5 1484.0 6 1796.0 7 1516.0 8 1254.0 9 1184.0 10 1071.0 11 1032.0 12 981.0 13 959.0 14 1043.0 15 1019.0 16 1072.0 17 1194.0 18 1436.0 19 1722.0 20 2331.0 21 2836.0 22 3506.0 23 4240.0 24 4856.0 25 5561.0 26 6200.0 27 6815.0 28 7731.0 29 8939.0 30 10657.0 31 12949.0 32 16853.0 33 21816.0 34 28850.0 35 40069.0 36 56544.0 37 85425.0 38 90871.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32256776709201 18.772640957452428 11.941963106988887 25.96282816846668 2 14.061088360704183 21.137754886646682 41.465571832650824 23.33558491999831 3 19.418492234038514 25.100583871961902 29.51445325857971 25.966470635419874 4 11.194979167765414 17.08116660513686 39.66921575866252 32.05463846843521 5 11.827841353584812 38.30806115929377 36.54818160539983 13.315915881721589 6 29.7812087499631 38.52083957135808 19.79917426124215 11.898777417436667 7 26.493226926509088 31.57652113968645 24.288541812045626 17.64171012175884 8 24.610271124013387 37.32305473859146 21.894590840182087 16.172083297213067 9 25.78776019702439 15.738124557201175 20.72045477547991 37.75366047029453 10 12.965962292800201 30.611160318866254 36.24804926399258 20.17482812434097 11 33.719471439204334 23.400854789159737 24.575293767882783 18.304380003753145 12 21.685527589238838 26.14783842652042 32.98753218164682 19.17910180259393 13 27.96201335942244 22.926506308616155 26.304314823561164 22.807165508400242 14 19.51794476037456 23.076955516055506 29.155254174389206 28.249845549180723 15 22.4961625396397 31.736851427029215 23.78803724445044 21.978948788880643 16 20.788271239190895 29.735183541274385 28.664025319818748 20.812519899715976 17 20.66955823157682 29.633128309151445 28.441570216740853 21.255743242530887 18 20.194825732177872 29.41004069411938 30.003795306470998 20.391338267231745 19 22.46357252809818 28.111675769141552 28.2112055331801 21.213546169580162 20 21.989512236778015 28.71568125127829 29.1926277813974 20.102178730546296 21 21.977822712426914 28.02502377342816 28.16882402181041 21.828329492334518 22 21.29725489865328 29.28628208399137 28.661312394177912 20.755150623177435 23 21.237040363850284 28.752944054012453 28.498021131824036 21.511994450313225 24 22.01223780647229 28.30361736402203 29.027258849426907 20.656885980078773 25 21.442984647888213 28.516426401162214 28.894272635456396 21.14631631549317 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 222.0 1 222.0 2 270.5 3 319.0 4 319.0 5 319.0 6 523.0 7 727.0 8 727.0 9 727.0 10 1099.0 11 1471.0 12 1471.0 13 1471.0 14 2276.0 15 3081.0 16 3081.0 17 3081.0 18 5059.0 19 7037.0 20 7037.0 21 7037.0 22 10536.0 23 14035.0 24 14035.0 25 14035.0 26 19736.5 27 25438.0 28 25438.0 29 25438.0 30 32573.0 31 39708.0 32 39708.0 33 39708.0 34 45279.5 35 50851.0 36 50851.0 37 50851.0 38 54684.5 39 58518.0 40 58518.0 41 58518.0 42 60526.5 43 62535.0 44 62535.0 45 62535.0 46 62366.5 47 62198.0 48 62198.0 49 62198.0 50 58675.0 51 55152.0 52 55152.0 53 55152.0 54 47711.5 55 40271.0 56 40271.0 57 40271.0 58 33076.5 59 25882.0 60 25882.0 61 25882.0 62 20225.5 63 14569.0 64 14569.0 65 14569.0 66 10850.0 67 7131.0 68 7131.0 69 7131.0 70 5158.0 71 3185.0 72 3185.0 73 3185.0 74 2199.0 75 1213.0 76 1213.0 77 1213.0 78 859.5 79 506.0 80 506.0 81 506.0 82 342.5 83 179.0 84 179.0 85 179.0 86 126.5 87 74.0 88 74.0 89 74.0 90 47.0 91 20.0 92 20.0 93 20.0 94 12.5 95 5.0 96 5.0 97 5.0 98 5.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19163792449170622 2 0.12480709713871296 3 0.12607203393403776 4 0.06472259936078528 5 0.0200281659259759 6 0.018974051929871904 7 0.30147660288574246 8 0.28988134892859857 9 0.01602253274078072 10 0.03204506548156144 11 0.013281836350910334 12 0.014968418744676725 13 0.012649367953247937 14 0.014968418744676725 15 0.012649367953247937 16 0.016655001138443117 17 0.016655001138443117 18 0.013071013551689534 19 0.02150392552052149 20 0.01370348194935193 21 0.013281836350910334 22 0.014546773146235125 23 0.014968418744676725 24 0.01349265915013113 25 0.01370348194935193 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 474332.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.82054995535505 #Duplication Level Percentage of deduplicated Percentage of total 1 96.3066544471222 88.42929975695101 2 2.916078034128598 5.3551177761283695 3 0.36954427755089403 1.0179527639273238 4 0.12752681774398292 0.46838330157235364 5 0.06502158595034956 0.29851588904652415 6 0.04109286572657335 0.22639017181533155 7 0.025573560806522955 0.1643724892300156 8 0.02363995495824985 0.17365069321490587 9 0.018227844902825602 0.15063216691305262 >10 0.0905632185396155 1.5430890947278904 >50 0.008957405901718918 0.5835346626080705 >100 0.006660632689825311 1.321862749020209 >500 4.5935397860850263E-4 0.2671984848449831 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 723 0.15242488383663763 No Hit GTCCTACAGTGGACATTTCTAAATT 544 0.11468760277611463 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATAAG 40 0.0050888406 14.326092 1 TAGGAGT 40 0.005179223 14.288144 4 GTGTTAT 55 1.8691986E-4 13.891969 1 TCTAAAC 70 6.9250564E-6 13.629417 3 CTAGCCA 50 0.0014669978 13.335601 4 TTAGACT 65 5.3021395E-5 13.189055 4 CTTAGAC 60 3.9383795E-4 12.72079 3 GTGTAGC 85 3.6998354E-6 12.359765 1 GTCCTAC 290 0.0 12.185411 1 CTAGACA 55 0.0030022874 12.123273 4 CTAAACA 80 2.779375E-5 11.906787 4 GTCTATG 65 7.655373E-4 11.754743 1 ACTGGAC 65 7.7239156E-4 11.742268 3 TAGCACT 65 7.8277336E-4 11.723605 4 CAGTCCT 75 2.0172725E-4 11.430514 4 TTGGACA 85 5.161945E-5 11.206387 4 GTGCAAG 60 0.005637359 11.142516 1 GGATAGG 60 0.005637359 11.142516 1 TCTGCGT 60 0.005947774 11.062598 10 AATCCCG 60 0.0059571206 11.060266 19 >>END_MODULE