FastQCFastQC Report
Thu 26 May 2016
SRR1926786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926786_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474332
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12270.2586795746439203No Hit
GGTATCAACGCAGAGTACTTTTTTT7660.16149026420313198No Hit
TATCAACGCAGAGTACTTTTTTTTT6280.1323967179106617No Hit
CACACACACACACACACACACACAC5470.11532007117377702No Hit
GTCCTACAGTGGACATTTCTAAATT5180.10920620999637384No Hit
CTGTAGGACGTGGAATATGGCAAGA5100.10751962760260747No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4760.10035165242910028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC250.006083902418.96858610
GGCGTGC307.798222E-418.9685868
GTATAAG453.4098113E-516.9574971
TCTATGC350.002125776416.3328233
TAGACTG456.603776E-414.8205255
GTGCGCC456.8352977E-414.75334411
GTCTTGC400.005131977614.3078881
CTTATAC500.001464620713.3384733
TAAGGCA500.001464620713.3384734
TAGGACC1700.012.8901214
CATCTAC603.9453653E-412.71812251
GGCGAGG905.4664633E-712.64972419
TGCGCCA604.1401054E-412.64572312
AGCTGCT853.981515E-612.2789697
GTCCTAG701.04739425E-412.2639041
ACAGGTC701.1047553E-412.194098
GGACCTG2500.012.1938836
ACTATAC550.002997454312.1258843
TGTAGGA3700.012.11659052
GACGTGG1353.7471182E-1011.9482217
Produced by FastQC (version 0.11.5)