FastQCFastQC Report
Thu 26 May 2016
SRR1926785_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926785_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639878
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11290.17643988385285947No Hit
GTCCTAAAGTGTGTATTTCTCATTT9480.14815324171170127No Hit
CTGTAGGACGTGGAATATGGCAAGA8580.1340880605365398No Hit
GTCCTACAGTGTGCATTTCTCATTT8350.13049362534733183No Hit
CTTTAGGACGTGAAATATGGCGAGG7010.10955213337542469No Hit
GTATCAACGCAGAGTACTTTTTTTT6630.10361350132368982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA2550.012.7191221
TAGGACC2150.012.3950734
TAAAGGT802.806348E-511.8970584
CTGTACT1052.6455018E-711.78375154
CTAGGAC657.8107323E-411.7278213
AAGGCGT657.9090643E-411.7103526
ACAGGTC657.9560187E-411.7020958
CCTACAC907.194092E-611.6463783
TCTAGGA907.194092E-611.6463782
GTCCTAG1001.8302017E-611.447211
ACTGACC752.0541414E-411.4095428
GTGCTAA600.005691384411.1292311
GTCTAGG951.2958442E-511.0455531
CTCCTAG700.001438829910.9021031
TTAGACT700.001466434710.8773094
GGCGAGA3250.010.79821219
ATACACT2400.010.7073524
TGGCGAG4550.010.63144418
GATATAC1355.325819E-810.5992691
ACTGTGT2100.010.4134718
Produced by FastQC (version 0.11.5)