##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926785_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 639878 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.60171782746086 30.0 27.0 31.0 10.0 31.0 2 27.286074845517426 31.0 28.0 31.0 12.0 34.0 3 26.896458387380093 31.0 27.0 31.0 10.0 34.0 4 30.64573715614539 35.0 32.0 37.0 10.0 37.0 5 30.154170013658852 35.0 30.0 37.0 10.0 37.0 6 29.989854315978985 35.0 30.0 37.0 10.0 37.0 7 30.031988910386044 35.0 30.0 37.0 10.0 37.0 8 29.75709432110496 35.0 30.0 37.0 10.0 37.0 9 32.02207764605128 37.0 32.0 39.0 10.0 39.0 10 32.22512260149591 37.0 32.0 39.0 10.0 39.0 11 32.44974354486324 37.0 32.0 39.0 10.0 39.0 12 32.319093639725075 37.0 32.0 39.0 8.0 39.0 13 32.43767718221286 37.0 33.0 39.0 8.0 39.0 14 33.366732408365344 38.0 33.0 40.0 7.0 41.0 15 32.9393868831246 38.0 32.0 40.0 2.0 41.0 16 33.00641997380751 38.0 32.0 40.0 2.0 41.0 17 33.23283657197153 38.0 33.0 40.0 2.0 41.0 18 33.27734505640138 38.0 33.0 40.0 2.0 41.0 19 32.63057645363647 38.0 32.0 40.0 2.0 41.0 20 32.9305180049947 39.0 32.0 40.0 2.0 41.0 21 32.91494941223171 39.0 32.0 40.0 2.0 41.0 22 32.92899896542779 39.0 33.0 40.0 2.0 41.0 23 32.91125183238055 39.0 33.0 40.0 2.0 41.0 24 31.58289080105895 38.0 30.0 40.0 2.0 41.0 25 32.14542615936163 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.946121519017133 1101 2 -0.9826295532762224 1101 3 -1.8736212363713918 1101 4 -1.1919517875870937 1101 5 -1.2665575043466397 1101 6 -3.212529430083194 1101 7 -1.6972002698331714 1101 8 -0.9292641402299822 1101 9 -1.1617774140287231 1101 10 -9.55125359061961 1101 11 -4.979810493843853 1101 12 -2.8743013701481033 1101 13 -2.129851647631366 1101 14 -1.8172055090197397 1101 15 -3.279333258921458 1101 16 -1.4775055254507556 1101 17 -1.302490717664515 1101 18 -1.4543048533560778 1101 19 -2.554889461317938 1101 20 -1.5998870399445515 1101 21 -1.6201993608519274 1101 22 -1.3736093498366522 1101 23 -1.5878253002698806 1101 24 -1.9032922829779366 1101 25 -1.5660468556338536 1102 1 -0.7244685290366277 1102 2 -0.5404645565253787 1102 3 -0.756674235419311 1102 4 -0.7820667926497364 1102 5 -0.6156832542975224 1102 6 -1.5416086339640458 1102 7 -0.364312751131628 1102 8 0.08496414708621458 1102 9 0.1479957494416766 1102 10 0.1262335260931522 1102 11 -0.13818047465115768 1102 12 0.01923960701711991 1102 13 -0.138314569455126 1102 14 -0.40132090700375045 1102 15 -0.0024370355003213717 1102 16 -0.03591479839509759 1102 17 -0.4531685070284368 1102 18 -0.6264421944873888 1102 19 0.1496034434288589 1102 20 -0.1969159498899984 1102 21 -0.40740977024577063 1102 22 -0.3236252178150707 1102 23 -0.7740095803925939 1102 24 -4.3308738519431955 1102 25 -2.6877618062919986 1103 1 -1.3678470483275547 1103 2 -1.3506835346275672 1103 3 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1.3105335871974333 1110 10 1.3409171062788232 1110 11 1.2050876950644707 1110 12 1.1484488358861853 1110 13 1.2631331359823719 1110 14 1.206049229768979 1110 15 1.6948993889709598 1110 16 1.4527202077298114 1110 17 0.9252485333757079 1110 18 1.1776093559747665 1110 19 -1.2233993811153177 1110 20 0.5123524020542831 1110 21 0.7284589036357971 1110 22 1.059424479989076 1110 23 1.0117927825056512 1110 24 0.4275324572178434 1110 25 0.48046865221554924 1111 1 0.3654300581354697 1111 2 0.11275468737656169 1111 3 0.8409141429977467 1111 4 0.7180896202804021 1111 5 0.621933544246712 1111 6 0.9641786444509677 1111 7 0.8603749341614702 1111 8 1.0809843069749867 1111 9 0.46680619332725826 1111 10 0.1866528763149944 1111 11 0.27794130252686955 1111 12 0.2870553464515808 1111 13 0.38727172345620176 1111 14 0.49301858061611625 1111 15 -0.5656564838636129 1111 16 -0.09700375952699858 1111 17 0.1444280498424888 1111 18 0.2724884726699841 1111 19 0.9561932326292109 1111 20 0.3433537346261488 1111 21 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0.9720458392123774 2210 23 1.0583947242532261 2210 24 1.2586004183828905 2210 25 1.0338667104679757 2211 1 0.5197533484271943 2211 2 0.27769968976776127 2211 3 0.11146890148651067 2211 4 0.44418718125600876 2211 5 0.5596284270778931 2211 6 -2.1614311116465927 2211 7 -0.10585653403556705 2211 8 0.38334203055222105 2211 9 0.43609361561325244 2211 10 0.3514783186870645 2211 11 0.20426363633844602 2211 12 0.13692622163092238 2211 13 0.14538745755471894 2211 14 0.3278583701904836 2211 15 0.9447691498044932 2211 16 0.239199014265445 2211 17 0.33674273181284065 2211 18 0.29896384330604775 2211 19 0.5201052364551302 2211 20 0.3682652602119916 2211 21 0.059403729588915155 2211 22 -2.66255041937157 2211 23 -1.244063244015777 2211 24 1.074546428328432 2211 25 0.6768456354028558 2212 1 -0.7193022084400873 2212 2 -0.4061432493392658 2212 3 -3.2138502022369586 2212 4 -1.750981047677005 2212 5 -0.3006638780716848 2212 6 0.2774893898527111 2212 7 0.3130304397325254 2212 8 0.14234280971780677 2212 9 0.12368032729837353 2212 10 0.32281919182474184 2212 11 -0.0010044674558571387 2212 12 -0.006743412161256401 2212 13 -0.19280723939709077 2212 14 -0.6523366573164253 2212 15 -1.5915187164623603 2212 16 -0.5427148997430038 2212 17 -0.4062799584334229 2212 18 -0.40461040172972673 2212 19 0.2332750237193153 2212 20 -0.8791155628701972 2212 21 -1.0797996326286388 2212 22 -0.4865439971582646 2212 23 -0.22910609812292648 2212 24 1.0605299477957644 2212 25 0.746710816145665 2213 1 0.1439224667720751 2213 2 -0.07118519207716645 2213 3 0.597830294672427 2213 4 -6.756423998396642 2213 5 -1.4268800668008197 2213 6 -3.14243614154125 2213 7 -0.7320565909777699 2213 8 -2.590740725450434 2213 9 -0.7734356621697991 2213 10 0.09396194780350697 2213 11 0.1304399673624559 2213 12 0.26797775432523707 2213 13 0.3072228945861326 2213 14 0.17214042390595807 2213 15 -2.528596134432007 2213 16 -0.631077155422318 2213 17 -0.0063539750297181286 2213 18 0.04429742971584005 2213 19 0.5702162737522727 2213 20 -1.2883049210540243 2213 21 -0.028070883678623204 2213 22 0.06038779833745167 2213 23 0.21111090422375867 2213 24 -0.4089697527477796 2213 25 -0.07759111129924179 2214 1 1.2607779270127502 2214 2 0.9832369343293976 2214 3 0.8237516023057943 2214 4 1.306313558504339 2214 5 1.863863289117635 2214 6 1.381939262211585 2214 7 1.8854336062789656 2214 8 -1.7424391164484376 2214 9 -0.24355795233100253 2214 10 0.38397667069760644 2214 11 0.9292320891117711 2214 12 -0.2276391800076496 2214 13 0.5981014607565243 2214 14 0.8562179144036861 2214 15 -0.3309112344124756 2214 16 0.8093139026730185 2214 17 0.9359262707524749 2214 18 0.8852676383903315 2214 19 1.8214744802633973 2214 20 -0.3281618986541943 2214 21 0.28554178401964947 2214 22 0.9484487330909985 2214 23 0.5313901885108265 2214 24 -0.7840767941972331 2214 25 0.17743420306459257 2215 1 1.6712697853306935 2215 2 0.932277279021914 2215 3 1.2379729665271597 2215 4 1.584514741053347 2215 5 1.921848292754813 2215 6 2.330120673436479 2215 7 1.8563539054601321 2215 8 0.5918538721923774 2215 9 1.0634813851431204 2215 10 0.6319072099324998 2215 11 0.5817492024984503 2215 12 0.5318407968636265 2215 13 0.9923015614783637 2215 14 0.8689918684819844 2215 15 1.1401974519136004 2215 16 1.3810866070544208 2215 17 0.9766673223630917 2215 18 0.33278969460803864 2215 19 1.4335163357835157 2215 20 -2.1478511531260587 2215 21 -0.7760999819699421 2215 22 0.07104213394526937 2215 23 0.3956115527321913 2215 24 0.710441326407846 2215 25 0.9062788862571054 2216 1 0.5037879012727231 2216 2 -0.4276864890940253 2216 3 0.46401463319382685 2216 4 0.04829795361373357 2216 5 0.5275095246388695 2216 6 -1.148321355549033 2216 7 -9.02332604623069 2216 8 -10.431515693025013 2216 9 -5.6376055713786215 2216 10 -3.1553814374104974 2216 11 -1.9041052661005793 2216 12 -1.4592069084503798 2216 13 -1.1865027863477238 2216 14 -1.2585745566387843 2216 15 -2.175276581902825 2216 16 -1.2400184654093493 2216 17 -1.4779371462359414 2216 18 -1.1656855469378584 2216 19 -0.852262649723734 2216 20 -1.6414501869424818 2216 21 -3.9371054167525514 2216 22 -2.641971151078888 2216 23 -2.962721780601143 2216 24 -2.8176232871187246 2216 25 -2.349729567849174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49917.0 3 12.0 4 395.0 5 1887.0 6 2271.0 7 1902.0 8 1517.0 9 1413.0 10 1423.0 11 1317.0 12 1325.0 13 1326.0 14 1290.0 15 1415.0 16 1505.0 17 1591.0 18 1860.0 19 2346.0 20 3015.0 21 3801.0 22 4727.0 23 5581.0 24 6591.0 25 7352.0 26 8353.0 27 9263.0 28 10388.0 29 12359.0 30 14897.0 31 17909.0 32 22750.0 33 29861.0 34 40180.0 35 55361.0 36 79404.0 37 118638.0 38 114736.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.10048866056885 18.665893998245835 12.188165643403083 26.045451697782234 2 14.18489877541568 20.50638893148524 41.723180727114546 23.58553156598453 3 19.326343791565606 24.88177763868242 29.475314920585244 26.31656364916673 4 11.381197153254194 17.06702613270114 39.23095327827706 32.3208234357676 5 12.0602119611092 37.9783974739738 36.421202363460154 13.540188201456843 6 30.279829807454835 37.97819759717137 19.67061927702557 12.07135331834822 7 26.671283768432495 31.1665337421871 24.38722859511099 17.774953894269412 8 24.904611402130122 37.40126202836508 21.413271198206147 16.280855371298646 9 25.97434061828856 15.481754266403655 20.479831688461093 38.06407342684669 10 13.628873651198639 29.739604341178342 36.016553009339596 20.614968998283416 11 33.96302502094457 22.843334625436086 24.9534217798507 18.240218573768647 12 22.46232286824492 25.35270046797483 32.75073541134581 19.43424125243444 13 28.62385607890027 22.65272470088075 25.82577231769551 22.897646902523462 14 19.964612669727106 22.796068004945475 28.36988513185063 28.86943419347679 15 22.594808008215157 31.848425975276378 23.325825696748794 22.230940319759675 16 21.26765296632357 28.646143505818543 28.739615327503067 21.34658820035482 17 21.09685779174801 29.170346357223575 28.4394621217213 21.29333372930711 18 20.82126562810159 28.403405476458794 30.155534680748303 20.619794214691314 19 23.282703469060134 27.404371456573834 27.525201605024535 21.7877234693415 20 22.727606650442265 28.259690485903683 28.597151820087312 20.415551043566744 21 22.332675303889253 27.55642876010685 27.271491069712045 22.839404866291858 22 22.15522065190548 29.017515231655583 27.669564510633244 21.15769960580569 23 21.535193567748713 28.66096883909368 28.06075876556795 21.743078827589656 24 22.902224966199583 27.855451440718365 28.27668669943809 20.965636893643957 25 21.94476920479909 28.128536327658537 28.378150262274378 21.548544205267994 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 274.0 1 274.0 2 343.0 3 412.0 4 412.0 5 412.0 6 556.5 7 701.0 8 701.0 9 701.0 10 1035.0 11 1369.0 12 1369.0 13 1369.0 14 2363.5 15 3358.0 16 3358.0 17 3358.0 18 5707.5 19 8057.0 20 8057.0 21 8057.0 22 12608.0 23 17159.0 24 17159.0 25 17159.0 26 24734.5 27 32310.0 28 32310.0 29 32310.0 30 42014.5 31 51719.0 32 51719.0 33 51719.0 34 59100.5 35 66482.0 36 66482.0 37 66482.0 38 72000.5 39 77519.0 40 77519.0 41 77519.0 42 80307.0 43 83095.0 44 83095.0 45 83095.0 46 83729.0 47 84363.0 48 84363.0 49 84363.0 50 79912.5 51 75462.0 52 75462.0 53 75462.0 54 65599.5 55 55737.0 56 55737.0 57 55737.0 58 47021.5 59 38306.0 60 38306.0 61 38306.0 62 30505.5 63 22705.0 64 22705.0 65 22705.0 66 17380.5 67 12056.0 68 12056.0 69 12056.0 70 8739.5 71 5423.0 72 5423.0 73 5423.0 74 3795.0 75 2167.0 76 2167.0 77 2167.0 78 1478.0 79 789.0 80 789.0 81 789.0 82 527.5 83 266.0 84 266.0 85 266.0 86 187.0 87 108.0 88 108.0 89 108.0 90 66.5 91 25.0 92 25.0 93 25.0 94 16.5 95 8.0 96 8.0 97 8.0 98 8.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21847914758750886 2 0.12564895183144287 3 0.12939966681148593 4 0.06423099403323758 5 0.021566611135247656 6 0.02062893239023689 7 0.2611435304854988 8 0.26551936462888237 9 0.01750333657353433 10 0.036882030637090196 11 0.014690300338502028 12 0.015627979083512794 13 0.012971222639315619 14 0.015784258874347923 15 0.013283782220985875 16 0.017659616364369458 17 0.016409378037688434 18 0.013283782220985875 19 0.021410331344412527 20 0.015784258874347923 21 0.013908901384326386 22 0.01437774075683177 23 0.01594053866518305 24 0.014534020547666898 25 0.014065181175161517 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 639878.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.92844888963938 #Duplication Level Percentage of deduplicated Percentage of total 1 94.73650615645425 84.2477054571725 2 4.184463628184989 7.442357197792076 3 0.5404399518134176 1.4418145989827602 4 0.18435375144379218 0.6557717265153024 5 0.08845917219673545 0.3933268486758599 6 0.055931646860772906 0.2984348759502961 7 0.034960441373991395 0.21762844767203596 8 0.027092178818542582 0.19274123514989877 9 0.017261374124336423 0.13815245039228957 >10 0.10803324449128483 1.754791532064339 >50 0.011425144052671388 0.681042887944501 >100 0.009842942387939419 1.6434087915225764 >500 0.001054600969090599 0.71635367009959 >1k 1.757668281817665E-4 0.17647028006599108 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1129 0.17643988385285947 No Hit GTCCTAAAGTGTGTATTTCTCATTT 948 0.14815324171170127 No Hit CTGTAGGACGTGGAATATGGCAAGA 858 0.1340880605365398 No Hit GTCCTACAGTGTGCATTTCTCATTT 835 0.13049362534733183 No Hit CTTTAGGACGTGAAATATGGCGAGG 701 0.10955213337542469 No Hit GTATCAACGCAGAGTACTTTTTTTT 663 0.10361350132368982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.1255958167025587E-4 3 0.0 0.0 0.0 0.0 3.1255958167025587E-4 4 0.0 0.0 0.0 0.0 3.1255958167025587E-4 5 0.0 0.0 0.0 0.0 3.1255958167025587E-4 6 0.0 0.0 0.0 0.0 3.1255958167025587E-4 7 0.0 0.0 0.0 0.0 3.1255958167025587E-4 8 0.0 0.0 0.0 0.0 3.1255958167025587E-4 9 0.0 0.0 0.0 0.0 4.688393725053838E-4 10 0.0 0.0 0.0 0.0 4.688393725053838E-4 11 0.0 0.0 0.0 0.0 4.688393725053838E-4 12 0.0 0.0 0.0 0.0 4.688393725053838E-4 13 0.0 0.0 0.0 0.0 4.688393725053838E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAA 255 0.0 12.719122 1 TAGGACC 215 0.0 12.395073 4 TAAAGGT 80 2.806348E-5 11.897058 4 CTGTACT 105 2.6455018E-7 11.7837515 4 CTAGGAC 65 7.8107323E-4 11.727821 3 AAGGCGT 65 7.9090643E-4 11.710352 6 ACAGGTC 65 7.9560187E-4 11.702095 8 CCTACAC 90 7.194092E-6 11.646378 3 TCTAGGA 90 7.194092E-6 11.646378 2 GTCCTAG 100 1.8302017E-6 11.44721 1 ACTGACC 75 2.0541414E-4 11.409542 8 GTGCTAA 60 0.0056913844 11.129231 1 GTCTAGG 95 1.2958442E-5 11.045553 1 CTCCTAG 70 0.0014388299 10.902103 1 TTAGACT 70 0.0014664347 10.877309 4 GGCGAGA 325 0.0 10.798212 19 ATACACT 240 0.0 10.707352 4 TGGCGAG 455 0.0 10.631444 18 GATATAC 135 5.325819E-8 10.599269 1 ACTGTGT 210 0.0 10.413471 8 >>END_MODULE