FastQCFastQC Report
Thu 26 May 2016
SRR1926785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639878
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11870.18550411172129688No Hit
GTCCTACAGTGGACATTTCTAAATT10860.16971985284694896No Hit
CTGTAGGACGTGGAATATGGCAAGA10180.15909282707016026No Hit
GTCCTACAGTGTGCATTTCTCATTT9190.1436211277774826No Hit
CTTTAGGACGTGAAATATGGCGAGG8700.1359634180265613No Hit
GTCCTAAAGTGTGTATTTCTCATTT8670.13549457865405592No Hit
GGTATCAACGCAGAGTACTTTTTTT7670.11986659957054313No Hit
CTGTAGGACCTGGAATATGGCGAGA7270.11361540793713802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGCG456.6602917E-414.8052095
CTAGTGC602.519141E-514.2764513
TAGGACC3300.013.5554194
TAGGACA2200.013.4112134
AAGGCGT500.001477070413.3246886
TCCAACG500.001510552813.28512118
TTAGAAC905.26843E-712.6901793
AGGACAT2450.012.4311965
GTAGGAC6850.012.3659783
AGGCGTG550.003083626312.0811597
CACGTTG550.003090855712.07738314
CTGTAGG6400.012.0599351
TGTAGGA7100.011.6743492
TGGCGAG5650.011.42083718
GTTCTTA600.005738279311.1169761
CCCTAAC600.005744988611.11523152
TGTAGAA2150.011.0783052
TCTTGGA951.31406305E-511.031662
GGCGAGA3450.011.00217119
TTAGGAC3550.010.9921983
Produced by FastQC (version 0.11.5)