FastQCFastQC Report
Thu 26 May 2016
SRR1926784_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926784_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648421
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10070.15530033728087153No Hit
GTCCTACAGTGTGCATTTCTCATTT7720.11905845122227689No Hit
CTGTAGGACGTGGAATATGGCAAGA7600.11720780172141247No Hit
GTCCTAAAGTGTGTATTTCTCATTT7110.10965098292621614No Hit
GTATCAACGCAGAGTACTTTTTTTT6780.10456169679883903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTG504.4053686E-617.159662
TCGCCTT508.357655E-515.27197551
TCCAACG551.98448E-413.79579718
GACGTGA1600.013.0817047
ACGTGAA1750.011.9502448
GTAGAAC2450.011.6723343
GTATCAA4800.011.5335241
GGGTATA751.9813413E-411.4539821
CTATTAT1251.7000275E-811.4539821
GGACGTG3600.011.3639046
CTAATAC1201.2115015E-711.121143
TAGGACC2800.010.8773134
TTAGGAC2550.010.8407753
GTGTAGG1501.9608706E-910.817651
AGGACGT3800.010.7658045
CCTACAC803.6476168E-410.7239563
TAAGACT909.317433E-510.5751654
AGGACTA909.354073E-510.5710745
TAGGACG3900.010.4938184
GTAGGAC5300.010.4316883
Produced by FastQC (version 0.11.5)