FastQCFastQC Report
Thu 26 May 2016
SRR1926784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926784_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648421
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12690.1957061847164111No Hit
GTCCTACAGTGGACATTTCTAAATT10920.16840910457866107No Hit
CTGTAGGACGTGGAATATGGCAAGA10000.15422079173870062No Hit
GTCCTACAGTGTGCATTTCTCATTT8420.12985390664398594No Hit
CACACACACACACACACACACACAC8280.12769481555964413No Hit
GGTATCAACGCAGAGTACTTTTTTT7550.11643669776271898No Hit
CTTTAGGACGTGAAATATGGCGAGG6970.10749189184187434No Hit
TATCAACGCAGAGTACTTTTTTTTT6960.10733767105013563No Hit
GTCCTAAAGTGTGTATTTCTCATTT6920.10672078788318082No Hit
CTGTAGGACCTGGAATATGGCGAGA6830.10533280075753253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAC307.774056E-418.97886718
GCCTCGA402.7815142E-416.6065116
GGCCTCG402.7815142E-416.6065115
TCGAACT350.002184542416.26760119
TAGGACC2450.015.9281864
TCCTATA1055.456968E-1215.4328812
CCTCGAA456.8120385E-414.76134317
ACCGTGC456.8160234E-414.7602038
AACTGCC653.4011573E-614.6002557
CCCTAGC551.8996882E-413.86698151
GGACACC551.9260793E-413.8444556
TCCAACG707.330711E-613.556334518
GCCTAGG500.001458619313.3469711
GCCGGTT500.00151051713.28520711
TGCGCCA500.001511395213.284183512
TCAGGAC1259.822543E-1112.947573
GGACTGT1051.933222E-812.6907516
GGACCTT604.021323E-412.6907516
CTAGGAC1301.891749E-1012.4495873
ATAGGAC853.840625E-612.3203533