FastQCFastQC Report
Thu 26 May 2016
SRR1926783_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926783_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583811
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8230.140970279765198No Hit
GTATCAACGCAGAGTACTTTTTTTT6770.11596218639251402No Hit
GTCCTACAGTGTGCATTTCTCATTT6660.11407801497402413No Hit
GTCCTAAAGTGTGTATTTCTCATTT6580.11270770848784965No Hit
CTGTAGGACGTGGAATATGGCAAGA6550.11219384355553425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATC250.006087498718.96753113
AGGCGTG350.002146901616.3095827
AAGGCGT400.005222860314.2708846
TAGGACC1650.013.8503364
GCTTAAT655.2214004E-513.2117271
TAGACAG751.4527837E-512.685235
GGCGAGG1155.4296834E-912.37012919
GTAGGAC4050.012.2417073
ACCTAAG550.002965319412.1441141
CGTGAAA1800.012.1181459
GCATTTC3500.011.651484513
ATTTCTC5250.011.56116215
TAGTGGT752.025363E-411.4265274
TATACTG1001.888995E-611.4167075
CATTTCT5900.011.41266814
TAGGACG3550.011.2655914
GTATAAG855.0770577E-511.2256511
GACGTGG1950.011.221557
CAGAACT855.1851246E-511.2024774
GGACGTG3500.011.144886
Produced by FastQC (version 0.11.5)