##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926783_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 583811 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.67198117198888 30.0 27.0 31.0 10.0 31.0 2 27.327736202298347 31.0 28.0 31.0 15.0 34.0 3 26.948688873625198 31.0 27.0 31.0 10.0 34.0 4 30.705983614560193 35.0 32.0 37.0 10.0 37.0 5 30.270162775281726 35.0 30.0 37.0 10.0 37.0 6 30.04228251951402 35.0 30.0 37.0 10.0 37.0 7 30.077161958236484 35.0 30.0 37.0 10.0 37.0 8 29.806887845552755 35.0 30.0 37.0 10.0 37.0 9 32.05468550609701 37.0 32.0 39.0 10.0 39.0 10 32.248832241941315 37.0 32.0 39.0 10.0 39.0 11 32.455070219643 37.0 32.0 39.0 10.0 39.0 12 32.32785781699899 37.0 32.0 39.0 8.0 39.0 13 32.42593236509761 37.0 32.0 39.0 8.0 39.0 14 33.376438607700095 38.0 33.0 40.0 8.0 41.0 15 32.92792701747655 38.0 32.0 40.0 2.0 41.0 16 33.005107817427216 38.0 32.0 40.0 2.0 41.0 17 33.23480201640599 38.0 33.0 40.0 2.0 41.0 18 33.27911772816888 38.0 33.0 40.0 2.0 41.0 19 32.635805080753876 38.0 32.0 40.0 2.0 41.0 20 32.9102740441684 39.0 32.0 40.0 2.0 41.0 21 32.89810229680496 39.0 32.0 40.0 2.0 41.0 22 32.915849478684024 39.0 33.0 40.0 2.0 41.0 23 32.896881011149155 39.0 33.0 40.0 2.0 41.0 24 31.555141989445215 38.0 30.0 40.0 2.0 41.0 25 32.11601014711953 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.3391306808111167 1101 2 -1.238241537547772 1101 3 -2.0787267650365386 1101 4 -1.414173486766746 1101 5 -1.549590243152835 1101 6 -3.8056860208500005 1101 7 -1.8266222835971178 1101 8 -1.25167768178202 1101 9 -1.430885866078981 1101 10 -9.849683423012756 1101 11 -4.989553386692471 1101 12 -2.6359031265086266 1101 13 -2.1282284423492435 1101 14 -1.8733911777016665 1101 15 -3.3983710094595914 1101 16 -1.6879552549289834 1101 17 -1.5744240855634928 1101 18 -1.2962003650295735 1101 19 -2.18462938111357 1101 20 -1.484398679944217 1101 21 -1.433488364572657 1101 22 -1.479703102366173 1101 23 -1.3730357415752685 1101 24 -1.3360766894710103 1101 25 -1.0397730968237333 1102 1 -0.8636695741551392 1102 2 -0.49338722052024053 1102 3 -1.1560991253680015 1102 4 -0.6774988008538863 1102 5 -0.944123790332732 1102 6 -1.2186237380379161 1102 7 -0.4908297147652547 1102 8 -0.01053894882291928 1102 9 0.016494513498670926 1102 10 -0.05203573522874194 1102 11 -0.012448334567366715 1102 12 0.20774830359027874 1102 13 -0.15776599915223954 1102 14 -0.37010328676341686 1102 15 0.042340030369331316 1102 16 -0.02440272886643413 1102 17 0.039743249373685785 1102 18 -0.2630808782613272 1102 19 0.22262803668915865 1102 20 0.19723724627871775 1102 21 -0.2494466644924671 1102 22 -0.24006129536269327 1102 23 -0.7030678185600294 1102 24 -4.400150466535965 1102 25 -2.5184686327766848 1103 1 -1.0209206846855814 1103 2 -1.001330779443677 1103 3 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0.5365965330667315 2215 4 0.9262307693095515 2215 5 1.314866537744642 2215 6 1.493025768766028 2215 7 1.094288004292995 2215 8 -0.3675240929417676 2215 9 0.019169292100443158 2215 10 -0.18236909256188483 2215 11 -0.07590262693302918 2215 12 -0.2145907176975541 2215 13 0.08465366149047782 2215 14 -0.045520283427443076 2215 15 0.15517512768855113 2215 16 0.18894236437282075 2215 17 -0.08135978180206394 2215 18 -0.8770871662110267 2215 19 0.5140397053958097 2215 20 -4.081558923351924 2215 21 -2.1469362845755064 2215 22 -1.1458644489374663 2215 23 -1.019276088893868 2215 24 -0.6681467225659183 2215 25 -0.611688687016251 2216 1 0.6633033962679917 2216 2 -0.17705154430909786 2216 3 0.7872637691081543 2216 4 0.4768339506909989 2216 5 0.8431751388620405 2216 6 -0.5244825049608863 2216 7 -8.57070612402984 2216 8 -9.770338939388513 2216 9 -4.964016359655723 2216 10 -2.6348085075293213 2216 11 -1.2968556179297934 2216 12 -1.2027050196796871 2216 13 -0.7784650887319344 2216 14 -0.6543242405819925 2216 15 -1.9826847349497854 2216 16 -0.7283203664908484 2216 17 -1.2364254378285366 2216 18 -0.7931577686806861 2216 19 -0.2821953040344667 2216 20 -0.9618834669624725 2216 21 -3.5811557073718454 2216 22 -2.1312920137928124 2216 23 -2.0241513602365266 2216 24 -2.112952957219491 2216 25 -1.688589864910277 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45143.0 3 7.0 4 346.0 5 1797.0 6 2093.0 7 1717.0 8 1387.0 9 1285.0 10 1262.0 11 1193.0 12 1144.0 13 1223.0 14 1270.0 15 1327.0 16 1321.0 17 1448.0 18 1690.0 19 2254.0 20 2764.0 21 3528.0 22 4464.0 23 5277.0 24 5948.0 25 6953.0 26 7936.0 27 8626.0 28 9563.0 29 11153.0 30 13395.0 31 16325.0 32 20591.0 33 27039.0 34 36288.0 35 49624.0 36 71058.0 37 106287.0 38 109085.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.65589847377645 18.07453106948943 11.97804819852064 26.291522258213472 2 14.668909834266271 20.114593623092965 40.98222615519702 24.23427038744375 3 19.59783913565426 24.324644143371636 28.963985594237695 27.113531126736408 4 11.688547660799166 16.53304947178783 38.5162063344321 33.26219653298091 5 12.535379217236962 37.630466217559054 36.057675833853025 13.776478731350956 6 31.257142171837017 37.29588593769542 19.116092523141923 12.330879367325645 7 27.25364102898907 30.9775671036875 23.82075674301216 17.948035124311268 8 25.46066759435826 36.7003384356371 21.035922280059786 16.803071689944854 9 26.20962353561969 15.431544610703554 20.056742060052013 38.302089793624745 10 13.848539630045323 29.477796721985655 35.50451091100697 21.169152736962054 11 34.58268946778731 22.570813788944584 24.336233155278098 18.510263587990007 12 22.75839029655137 24.910143735759195 32.21531239827996 20.116153569409466 13 28.81765635011187 22.189329852224173 25.58923597191814 23.403777825745816 14 20.38830793267913 22.142769036785264 27.90143084260731 29.567492187928295 15 23.03932653564674 31.088280843132416 23.13286325137735 22.73952936984349 16 21.80861270250267 28.45607987719635 27.89311559441901 21.842191825881965 17 21.489940911528336 28.80927049979356 27.88002761687919 21.820760971798915 18 21.371524506192934 28.02991108903089 29.41394137700649 21.18462302776969 19 23.519736842105264 27.223478618421055 27.105948464912284 22.150836074561404 20 23.160091209519777 27.72741990471312 28.075366063000356 21.037122822766747 21 22.68864950799002 27.246080366197955 27.020461584847737 23.044808540964283 22 22.669492251342266 28.32431636040938 27.346268891957365 21.659922496290992 23 22.089847832269427 28.182381985204678 27.567337904190182 22.160432278335715 24 23.290909807321015 27.367714149151045 27.790007829136382 21.551368214391566 25 22.385807063918577 27.7737709579992 27.779766911587096 22.060655066495123 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 219.0 1 219.0 2 286.0 3 353.0 4 353.0 5 353.0 6 518.5 7 684.0 8 684.0 9 684.0 10 964.0 11 1244.0 12 1244.0 13 1244.0 14 1960.5 15 2677.0 16 2677.0 17 2677.0 18 4699.5 19 6722.0 20 6722.0 21 6722.0 22 10530.5 23 14339.0 24 14339.0 25 14339.0 26 20256.5 27 26174.0 28 26174.0 29 26174.0 30 34333.0 31 42492.0 32 42492.0 33 42492.0 34 49248.5 35 56005.0 36 56005.0 37 56005.0 38 61812.5 39 67620.0 40 67620.0 41 67620.0 42 70978.5 43 74337.0 44 74337.0 45 74337.0 46 75931.5 47 77526.0 48 77526.0 49 77526.0 50 74478.5 51 71431.0 52 71431.0 53 71431.0 54 63546.0 55 55661.0 56 55661.0 57 55661.0 58 47311.0 59 38961.0 60 38961.0 61 38961.0 62 31616.0 63 24271.0 64 24271.0 65 24271.0 66 18655.5 67 13040.0 68 13040.0 69 13040.0 70 9590.0 71 6140.0 72 6140.0 73 6140.0 74 4326.5 75 2513.0 76 2513.0 77 2513.0 78 1712.5 79 912.0 80 912.0 81 912.0 82 624.0 83 336.0 84 336.0 85 336.0 86 223.0 87 110.0 88 110.0 89 110.0 90 69.0 91 28.0 92 28.0 93 28.0 94 19.5 95 11.0 96 11.0 97 11.0 98 8.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21616584819402168 2 0.12110083571566826 3 0.12178598895875549 4 0.07331139701033382 5 0.02312392195419408 6 0.022267480400335042 7 0.3021525802014693 8 0.2947871828382816 9 0.020040732360301537 10 0.038711158234428604 11 0.016614966144865375 12 0.01730011938795261 13 0.015244659658690912 14 0.01695754276640899 15 0.014216929794060064 16 0.01969815573875792 17 0.01884171418489888 18 0.013874353172516448 19 0.02243876871110685 20 0.0171288310771808 21 0.014216929794060064 22 0.016614966144865375 23 0.019355579117214302 24 0.016101101212549953 25 0.014388218104831872 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 583811.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.59735770178986 #Duplication Level Percentage of deduplicated Percentage of total 1 94.78812200742811 84.92765273380436 2 4.022368128543864 7.207871120428472 3 0.6362006437341854 1.7100568994028231 4 0.19425232997964037 0.6961798197436783 5 0.0945979322999673 0.4237862389064935 6 0.06032594830750269 0.32430273415242017 7 0.04297503834172359 0.26953149177860797 8 0.024960748064111877 0.17891336582435924 9 0.016061428914622804 0.12951554324987968 >10 0.10234431731944091 1.672700002061972 >50 0.009182723334720507 0.5803792588151381 >100 0.007460919901294774 1.1898921022780975 >500 0.0011478338308163615 0.6892186895537273 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 823 0.140970279765198 No Hit GTATCAACGCAGAGTACTTTTTTTT 677 0.11596218639251402 No Hit GTCCTACAGTGTGCATTTCTCATTT 666 0.11407801497402413 No Hit GTCCTAAAGTGTGTATTTCTCATTT 658 0.11270770848784965 No Hit CTGTAGGACGTGGAATATGGCAAGA 655 0.11219384355553425 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.71288310771808E-4 3 0.0 0.0 0.0 0.0 1.71288310771808E-4 4 0.0 0.0 0.0 0.0 1.71288310771808E-4 5 0.0 0.0 0.0 0.0 1.71288310771808E-4 6 0.0 0.0 0.0 0.0 1.71288310771808E-4 7 0.0 0.0 0.0 0.0 1.71288310771808E-4 8 0.0 0.0 0.0 0.0 1.71288310771808E-4 9 0.0 0.0 0.0 0.0 1.71288310771808E-4 10 0.0 0.0 0.0 0.0 1.71288310771808E-4 11 0.0 0.0 0.0 0.0 1.71288310771808E-4 12 0.0 0.0 0.0 0.0 1.71288310771808E-4 13 0.0 1.71288310771808E-4 0.0 0.0 1.71288310771808E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAAATC 25 0.0060874987 18.967531 13 AGGCGTG 35 0.0021469016 16.309582 7 AAGGCGT 40 0.0052228603 14.270884 6 TAGGACC 165 0.0 13.850336 4 GCTTAAT 65 5.2214004E-5 13.211727 1 TAGACAG 75 1.4527837E-5 12.68523 5 GGCGAGG 115 5.4296834E-9 12.370129 19 GTAGGAC 405 0.0 12.241707 3 ACCTAAG 55 0.0029653194 12.144114 1 CGTGAAA 180 0.0 12.118145 9 GCATTTC 350 0.0 11.6514845 13 ATTTCTC 525 0.0 11.561162 15 TAGTGGT 75 2.025363E-4 11.426527 4 TATACTG 100 1.888995E-6 11.416707 5 CATTTCT 590 0.0 11.412668 14 TAGGACG 355 0.0 11.265591 4 GTATAAG 85 5.0770577E-5 11.225651 1 GACGTGG 195 0.0 11.22155 7 CAGAACT 85 5.1851246E-5 11.202477 4 GGACGTG 350 0.0 11.14488 6 >>END_MODULE