FastQCFastQC Report
Thu 26 May 2016
SRR1926783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926783_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583811
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12150.20811529758774672No Hit
GTCCTACAGTGGACATTTCTAAATT8760.1500485602361038No Hit
CTGTAGGACGTGGAATATGGCAAGA8380.1435396044267751No Hit
GGTATCAACGCAGAGTACTTTTTTT7530.1289800980111714No Hit
GTCCTACAGTGTGCATTTCTCATTT7250.1241840253095608No Hit
CTTTAGGACGTGAAATATGGCGAGG6930.11870279936486296No Hit
GTCCTAAAGTGTGTATTTCTCATTT6850.11733249287868848No Hit
TATCAACGCAGAGTACTTTTTTTTT6290.10774034747546724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT250.00594373119.0461716
GTACAAT350.00211482416.3463691
GTAGAAC1500.013.3334663
TAGGACC2100.012.6974474
ACAGATC604.134961E-412.648518
AGGACGT4200.012.4707075
GGACGTG4000.012.3800126
CTAGGAC701.0633959E-412.2450193
GGCGAGG1650.012.07564519
GACGTGA2250.011.808317
GATATAC1052.5808185E-711.8057111
GACGTGG2100.011.7480637
GGCTTAT657.7641814E-411.7358551
GTACAAG907.136421E-611.6543561
TTAGGAC2800.011.564743
TAGGACG4550.011.5114214
CCTCTAG751.999311E-411.4424581
TATTAGG752.0025125E-411.4404892
TGTAGGA5000.011.2498142
GTCCTAC4750.011.24171451
Produced by FastQC (version 0.11.5)