##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926783_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 583811 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.700593171420202 31.0 30.0 33.0 25.0 34.0 2 29.98152655568326 31.0 30.0 33.0 26.0 34.0 3 30.043104703405724 31.0 30.0 34.0 26.0 34.0 4 33.77339241638133 35.0 35.0 37.0 28.0 37.0 5 33.78246555820291 37.0 35.0 37.0 30.0 37.0 6 33.742039803977654 37.0 35.0 37.0 29.0 37.0 7 33.718348917714806 37.0 35.0 37.0 29.0 37.0 8 33.74249029223499 37.0 35.0 37.0 29.0 37.0 9 35.26117527761553 39.0 35.0 39.0 29.0 39.0 10 35.13805666559897 39.0 35.0 39.0 29.0 39.0 11 35.20755175904531 39.0 35.0 39.0 29.0 39.0 12 35.09145596777039 39.0 35.0 39.0 29.0 39.0 13 35.10500658603555 39.0 35.0 39.0 29.0 39.0 14 36.18494684067275 40.0 36.0 41.0 29.0 41.0 15 36.1610332796059 40.0 36.0 41.0 29.0 41.0 16 36.14400036998275 40.0 36.0 41.0 29.0 41.0 17 36.12050646527729 40.0 36.0 41.0 29.0 41.0 18 36.08562531367172 40.0 36.0 41.0 28.0 41.0 19 36.16242071492315 40.0 37.0 41.0 28.0 41.0 20 36.12943229915161 40.0 37.0 41.0 28.0 41.0 21 36.08045240668641 40.0 37.0 41.0 28.0 41.0 22 35.993944958214215 40.0 36.0 41.0 28.0 41.0 23 35.92568827925476 40.0 36.0 41.0 27.0 41.0 24 35.84566580622838 40.0 36.0 41.0 27.0 41.0 25 35.73675898535656 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3950516326356919 1101 2 -0.493755614097541 1101 3 -0.2865178285862733 1101 4 -0.30357245670516875 1101 5 -0.21433253963427745 1101 6 -0.3701416863331204 1101 7 -0.11198310693912106 1101 8 -0.3445682383686943 1101 9 -0.29639969895465157 1101 10 -0.21118351540505387 1101 11 -0.09145141981235838 1101 12 -0.32974956395797506 1101 13 -0.22755705741555943 1101 14 -0.35484050220308205 1101 15 -0.3449523969082122 1101 16 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2 -0.895060018075327 1103 3 -0.7150080778436703 1103 4 -0.760307572293442 1103 5 -0.6037294142306848 1103 6 -0.6011115946373948 1103 7 -0.6743491149463523 1103 8 -0.5652320637593675 1103 9 -0.47647202234352193 1103 10 -0.43678082910775373 1103 11 -0.4866341655181543 1103 12 -0.4956025924998855 1103 13 -0.32259773932180025 1103 14 -0.4012049604234065 1103 15 -0.3189934662611762 1103 16 -0.42554759422856137 1103 17 -0.7103502674551336 1103 18 -0.49322533523204015 1103 19 -0.349669387461347 1103 20 -0.4364770162711409 1103 21 -0.49600548568376723 1103 22 -0.33242111759778226 1103 23 -0.37781969848307284 1103 24 -0.3055593039271187 1103 25 -0.1369887377352228 1104 1 -0.5084171341852617 1104 2 -0.5239858914503337 1104 3 -0.12259330620582531 1104 4 -0.2071815736484055 1104 5 -0.48305091082411167 1104 6 -0.2943776911975817 1104 7 -0.3993865042165936 1104 8 -0.6207637583561407 1104 9 -0.47691046746118104 1104 10 -0.6986143733549213 1104 11 -0.4386876992835411 1104 12 -0.3092992089307103 1104 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13 0.16198505677324704 2208 14 0.06500121628193511 2208 15 0.028799251857570596 2208 16 -0.051875340283700666 2208 17 -0.12504910702043048 2208 18 -0.029499664451662966 2208 19 -0.2481221411913097 2208 20 -0.2552343073621941 2208 21 0.13711909220043594 2208 22 0.09111912922483612 2208 23 0.10331249283515831 2208 24 0.14272460348369975 2208 25 0.11500978869690215 2209 1 0.21388581283299146 2209 2 0.32273086213628943 2209 3 0.1931908319349489 2209 4 0.4109338215088627 2209 5 0.14700049022042805 2209 6 8.723116184015112E-4 2209 7 0.14391765555092206 2209 8 0.022470189612036506 2209 9 0.12762063411313562 2209 10 -0.06445539431708625 2209 11 -0.07966886224625114 2209 12 -0.10250856399591157 2209 13 -0.07276399190211436 2209 14 -0.03214030114849464 2209 15 0.00493948883208617 2209 16 0.12368418288173189 2209 17 0.12359739697423322 2209 18 0.04234653656187959 2209 19 -0.015526422603059586 2209 20 0.24114254343763974 2209 21 0.22438486795833512 2209 22 0.10486148706718268 2209 23 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2211 10 0.02746621754431544 2211 11 0.10910483311043606 2211 12 0.22704976656947906 2211 13 0.21326107741423783 2211 14 0.1898640191978913 2211 15 0.2706054558570514 2211 16 0.3776234954425419 2211 17 0.3470469367890274 2211 18 0.29279588556530456 2211 19 0.20563220289546535 2211 20 0.3063423182331064 2211 21 0.18616134539194462 2211 22 0.32928039576746926 2211 23 0.24720736886047945 2211 24 0.4713961612702988 2211 25 0.3318104346055577 2212 1 0.06707847252449284 2212 2 0.012028196125005053 2212 3 0.1988575688214027 2212 4 0.14192766557411574 2212 5 0.16388951954889563 2212 6 0.02403781965856666 2212 7 -0.13727046326096115 2212 8 -0.18219357463673447 2212 9 -0.1723840472060587 2212 10 -0.11196610283474939 2212 11 -0.14304105052891458 2212 12 -0.03574124896653785 2212 13 -0.07601633841336053 2212 14 0.027685787843381604 2212 15 -0.21531189800872852 2212 16 -0.17254735516569042 2212 17 -0.11662566788544382 2212 18 -0.1372487633936501 2212 19 -0.30516783355266597 2212 20 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0.4057821924720528 2214 7 0.23894463498102425 2214 8 0.3506795358827546 2214 9 0.26364974891706794 2214 10 -0.29710989037682367 2214 11 0.12555341799624387 2214 12 0.05628497823317957 2214 13 0.24990612412707236 2214 14 0.09734958682685857 2214 15 0.138038010680134 2214 16 0.17660664162191608 2214 17 0.2584353895635658 2214 18 0.07425313983854664 2214 19 0.3009412117508603 2214 20 0.24722329629059203 2214 21 0.24830856332683737 2214 22 -0.008428228667575866 2214 23 0.24845407770413885 2214 24 0.2398736928310754 2214 25 -0.04236386990584151 2215 1 -0.052565600110160204 2215 2 -0.14731242702234937 2215 3 -0.007894292057326169 2215 4 -0.0022273088456614687 2215 5 -0.10518695762652186 2215 6 -0.12291560896932197 2215 7 -0.15522711960770863 2215 8 -0.265656455869852 2215 9 -0.4030649421844288 2215 10 -0.3865900845662935 2215 11 -0.5542249865893396 2215 12 -0.4961742463536183 2215 13 -0.5949994971863859 2215 14 -0.5049525978580434 2215 15 -0.5520090852822932 2215 16 -0.5330440286391109 2215 17 -0.4802369802715418 2215 18 -0.5617739374050217 2215 19 -0.6711000294993568 2215 20 -0.5708782871283873 2215 21 -0.5851939227380711 2215 22 -0.41602868244512337 2215 23 -0.34324217735043305 2215 24 -0.35578831333099714 2215 25 -0.5412523710418853 2216 1 -0.08991302546868951 2216 2 0.14829982876926096 2216 3 -1.6598105939682881 2216 4 -1.3989747555557415 2216 5 -0.37464356059438586 2216 6 -0.015036885792213184 2216 7 -0.20684449977211727 2216 8 -0.25700907677302354 2216 9 -0.1794963859052956 2216 10 -0.3936716830859197 2216 11 -0.2777935428684728 2216 12 0.01048032109949304 2216 13 -0.27015340629994 2216 14 -0.32353556643567316 2216 15 -0.2506319100927996 2216 16 -0.17754066576946315 2216 17 -0.4711269841859931 2216 18 -0.384111220775722 2216 19 -0.4187602551141012 2216 20 -0.1899309898791799 2216 21 -0.235775752566191 2216 22 -0.40971218125299913 2216 23 -0.3391320033352727 2216 24 -0.35532569634227684 2216 25 -0.40901508411259613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14322.0 3 12.0 4 203.0 5 1641.0 6 2177.0 7 1540.0 8 1245.0 9 1082.0 10 984.0 11 860.0 12 808.0 13 729.0 14 696.0 15 749.0 16 742.0 17 794.0 18 923.0 19 1088.0 20 1315.0 21 1795.0 22 2304.0 23 2768.0 24 3233.0 25 3962.0 26 4605.0 27 5115.0 28 5963.0 29 7350.0 30 9388.0 31 12012.0 32 15629.0 33 20803.0 34 28130.0 35 39305.0 36 58728.0 37 102856.0 38 227955.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26816853485532 19.057582284906776 9.877104284714695 28.797144895523207 2 14.663923182441701 21.22153635116598 39.14334705075446 24.97119341563786 3 18.578046089643877 24.801107357485968 29.183940575469563 27.43690597740059 4 12.008167026657599 17.312966011260496 38.02788916275986 32.65097779932204 5 12.828032285913968 38.63278198785852 35.60854699683445 12.930638729393063 6 31.627494426271213 38.41738762726752 17.61006073086754 12.34505721559372 7 28.26858271615091 31.81286309257651 21.866160928283488 18.0523932629891 8 25.26132225119141 38.00538575652406 19.78460399957517 16.948687992709356 9 26.464597669251628 15.391059012164224 19.449911864615828 38.69443145396832 10 13.978317805675603 29.963497585633363 34.73690340991817 21.321281198772862 11 34.86778658800838 22.78075544158783 23.985809990219103 18.36564798018469 12 22.862100447235314 25.228456567130685 31.945036818548534 19.964406167085468 13 28.92589871002331 22.173519079013857 25.544998449835475 23.355583761127356 14 20.6381859882736 22.38412773997064 27.479783697121157 29.497902574634598 15 22.83953895090605 31.32105399289824 23.09887478395818 22.740532272237534 16 21.990164625195483 28.67434837644375 27.425656590917264 21.909830407443504 17 21.509187048548245 29.01504082656887 27.667344397416286 21.808427727466594 18 21.424436032270773 28.189822031140267 29.224747777530364 21.160994159058596 19 23.071336442787135 27.410069354637027 27.352002617285386 22.166591585290448 20 23.194361179150754 27.837995238176806 28.013908634658534 20.953734948013906 21 22.536137787843177 27.051177069164588 27.3439454001624 23.06873974282983 22 22.673604985174997 28.258289069579025 27.29530618641992 21.772799758826057 23 22.017913331540516 28.138729828660303 27.56525656419629 22.278100275602892 24 22.624102663361946 27.223707672517307 28.364316533946777 21.787873130173978 25 22.337163569588338 27.807755029326316 27.81683385408102 22.03824754700433 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 182.0 1 182.0 2 179.5 3 177.0 4 177.0 5 177.0 6 344.0 7 511.0 8 511.0 9 511.0 10 853.0 11 1195.0 12 1195.0 13 1195.0 14 1874.0 15 2553.0 16 2553.0 17 2553.0 18 4555.0 19 6557.0 20 6557.0 21 6557.0 22 10154.0 23 13751.0 24 13751.0 25 13751.0 26 19952.0 27 26153.0 28 26153.0 29 26153.0 30 34206.0 31 42259.0 32 42259.0 33 42259.0 34 49059.5 35 55860.0 36 55860.0 37 55860.0 38 61425.5 39 66991.0 40 66991.0 41 66991.0 42 70507.5 43 74024.0 44 74024.0 45 74024.0 46 76161.5 47 78299.0 48 78299.0 49 78299.0 50 75439.0 51 72579.0 52 72579.0 53 72579.0 54 64156.5 55 55734.0 56 55734.0 57 55734.0 58 47739.0 59 39744.0 60 39744.0 61 39744.0 62 32182.0 63 24620.0 64 24620.0 65 24620.0 66 18816.0 67 13012.0 68 13012.0 69 13012.0 70 9491.0 71 5970.0 72 5970.0 73 5970.0 74 4176.0 75 2382.0 76 2382.0 77 2382.0 78 1638.0 79 894.0 80 894.0 81 894.0 82 581.5 83 269.0 84 269.0 85 269.0 86 172.0 87 75.0 88 75.0 89 75.0 90 46.0 91 17.0 92 17.0 93 17.0 94 10.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12247114220184273 2 0.10465715788157469 3 0.01353177655097283 4 0.0 5 0.004624784390838816 6 0.3535390734330117 7 0.01884171418489888 8 0.008393127227818592 9 0.0085644155385904 10 0.002398036350805312 11 0.00342576621543616 12 3.42576621543616E-4 13 3.42576621543616E-4 14 0.0 15 0.002055459729261696 16 0.0 17 0.0 18 1.71288310771808E-4 19 0.0 20 1.71288310771808E-4 21 0.012504046686341985 22 0.0 23 0.0 24 0.0 25 0.00599509087701328 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 583811.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.74200701404101 #Duplication Level Percentage of deduplicated Percentage of total 1 93.00167678813757 80.67152100274204 2 5.460761367474289 9.473548016789179 3 0.795917488165794 2.0711844102322567 4 0.29030365358978705 1.0072608622354815 5 0.13122656443564526 0.5691427786352623 6 0.06937641465071322 0.36107096677447104 7 0.05527578045933035 0.3356312495416877 8 0.03374499723084453 0.23416870291893688 9 0.019325070018512174 0.1508665823183355 >10 0.12222847972765108 1.9869882068207951 >50 0.010279399166263676 0.666854879144238 >100 0.00810487749390493 1.2814068665335503 >500 0.0015814395108637296 0.982017546401913 >1k 1.976799388579662E-4 0.20833792891187783 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1215 0.20811529758774672 No Hit GTCCTACAGTGGACATTTCTAAATT 876 0.1500485602361038 No Hit CTGTAGGACGTGGAATATGGCAAGA 838 0.1435396044267751 No Hit GGTATCAACGCAGAGTACTTTTTTT 753 0.1289800980111714 No Hit GTCCTACAGTGTGCATTTCTCATTT 725 0.1241840253095608 No Hit CTTTAGGACGTGAAATATGGCGAGG 693 0.11870279936486296 No Hit GTCCTAAAGTGTGTATTTCTCATTT 685 0.11733249287868848 No Hit TATCAACGCAGAGTACTTTTTTTTT 629 0.10774034747546724 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACCGT 25 0.005943731 19.046171 6 GTACAAT 35 0.002114824 16.346369 1 GTAGAAC 150 0.0 13.333466 3 TAGGACC 210 0.0 12.697447 4 ACAGATC 60 4.134961E-4 12.64851 8 AGGACGT 420 0.0 12.470707 5 GGACGTG 400 0.0 12.380012 6 CTAGGAC 70 1.0633959E-4 12.245019 3 GGCGAGG 165 0.0 12.075645 19 GACGTGA 225 0.0 11.80831 7 GATATAC 105 2.5808185E-7 11.805711 1 GACGTGG 210 0.0 11.748063 7 GGCTTAT 65 7.7641814E-4 11.735855 1 GTACAAG 90 7.136421E-6 11.654356 1 TTAGGAC 280 0.0 11.56474 3 TAGGACG 455 0.0 11.511421 4 CCTCTAG 75 1.999311E-4 11.442458 1 TATTAGG 75 2.0025125E-4 11.440489 2 TGTAGGA 500 0.0 11.249814 2 GTCCTAC 475 0.0 11.2417145 1 >>END_MODULE