FastQCFastQC Report
Thu 26 May 2016
SRR1926782_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926782_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848665
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17790.2096233496138052No Hit
GTCCTACAGTGTGCATTTCTCATTT14440.1701495878821443No Hit
CTGTAGGACGTGGAATATGGCAAGA14250.167910777515274No Hit
GTCCTAAAGTGTGTATTTCTCATTT13330.15707022205463875No Hit
CTGTAGGACCTGGAATATGGCGAGA10480.12348806655158395No Hit
CTTTAGGACGTGAAATATGGCGAGG10280.1211314240601415No Hit
GTATCAACGCAGAGTACTTTTTTTT8650.10192478775488562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTT751.6189006E-1017.803181
CGCCTTG751.6370905E-1017.7894762
CCAACGT453.5835856E-516.8624919
TCCAACG902.3337634E-914.75467918
CGCGTGG400.005333615514.22688811
CCCGATC400.005333615514.22688812
CCGGTTT551.9856541E-413.79576912
TAGGACC4000.012.6101814
GGCGAGG3400.012.55387619
GTCCTAC10250.012.1892841
GTAGGAC10000.012.0078973
TGTAGGA10250.011.993952
TGGCGAG8350.011.92743318
GGCGTGC657.967426E-411.7009228
CTGTAGG10550.011.4810621
AGGACGT7700.011.3670685
AGGACCT6200.011.3551115
CCTATAC855.14751E-511.2118563
GGACGTG7850.011.1498636
CTAGTAC600.00573534311.1184233
Produced by FastQC (version 0.11.5)