##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926782_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 848665 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.7191871940047 30.0 27.0 31.0 10.0 31.0 2 27.37528353354975 31.0 28.0 31.0 15.0 34.0 3 27.003619802866854 31.0 27.0 31.0 10.0 34.0 4 30.746719848232225 35.0 32.0 37.0 12.0 37.0 5 30.335191153164086 35.0 30.0 37.0 10.0 37.0 6 30.16021751810196 35.0 30.0 37.0 10.0 37.0 7 30.232047981241127 35.0 30.0 37.0 10.0 37.0 8 29.915914995905332 35.0 30.0 37.0 10.0 37.0 9 32.14036516175405 37.0 32.0 39.0 10.0 39.0 10 32.319469991103674 37.0 32.0 39.0 10.0 39.0 11 32.53274613657921 37.0 32.0 39.0 10.0 39.0 12 32.388640983191245 37.0 32.0 39.0 10.0 39.0 13 32.50097270418834 37.0 33.0 39.0 10.0 39.0 14 33.450306068943576 38.0 33.0 40.0 8.0 41.0 15 33.01837120654204 38.0 32.0 40.0 7.0 41.0 16 33.08496874502896 38.0 32.0 40.0 2.0 41.0 17 33.313967230886156 38.0 33.0 40.0 2.0 41.0 18 33.352270919620814 39.0 33.0 40.0 2.0 41.0 19 32.73498612526733 38.0 32.0 40.0 2.0 41.0 20 33.01244542899731 39.0 32.0 40.0 2.0 41.0 21 32.99701413396334 39.0 32.0 40.0 2.0 41.0 22 33.010106461324554 39.0 33.0 40.0 2.0 41.0 23 32.9943252048806 39.0 33.0 40.0 2.0 41.0 24 31.686055157217513 38.0 31.0 40.0 2.0 41.0 25 32.24182333429563 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.9844805094055928 1101 2 -1.1713359451781642 1101 3 -1.9251813034393024 1101 4 -1.5052370785273617 1101 5 -1.265247223183671 1101 6 -3.019017450788944 1101 7 -1.5138515581532914 1101 8 -0.9708904231178366 1101 9 -1.290454930739454 1101 10 -9.837117200425489 1101 11 -4.757544496824984 1101 12 -2.6982803010448784 1101 13 -1.6668145177292786 1101 14 -1.414272705357785 1101 15 -2.6904267101431536 1101 16 -1.3692579898721071 1101 17 -1.1604979988351616 1101 18 -1.02236914901097 1101 19 -1.92954307850124 1101 20 -1.2747590471990584 1101 21 -1.2853374097145114 1101 22 -0.9224621753193816 1101 23 -1.1514404714590682 1101 24 -1.3189009423644684 1101 25 -1.1836586158537123 1102 1 -1.1690446424870196 1102 2 -1.1795307736241227 1102 3 -1.4042980358109354 1102 4 -1.1762518618278541 1102 5 -1.2573810253125366 1102 6 -2.073212946646805 1102 7 -1.205522486020783 1102 8 -0.7851081998748981 1102 9 -0.8444385296629662 1102 10 -0.8361395561864811 1102 11 -1.1428809751994429 1102 12 -1.0734895016858985 1102 13 -1.3324678010532445 1102 14 -1.5536019183319674 1102 15 -0.8906109462800842 1102 16 -1.0535746983894754 1102 17 -1.3429044628336193 1102 18 -1.5536582056128267 1102 19 -0.9837747364107692 1102 20 -1.095631275885335 1102 21 -1.2043014240321455 1102 22 -1.3847984468722778 1102 23 -1.6507819414655032 1102 24 -4.898071727487018 1102 25 -3.174034434733855 1103 1 -0.846123499687149 1103 2 -0.9451559497984618 1103 3 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21 1.0837566777306478 1111 22 1.2768467892984887 1111 23 1.085635900848871 1111 24 1.558974334843196 1111 25 1.327179156071324 1112 1 0.6150848571733825 1112 2 0.6772386435060405 1112 3 -1.1036869945901628 1112 4 0.4810294015197094 1112 5 0.6702381312265153 1112 6 1.6513108589870065 1112 7 1.3672026224518703 1112 8 1.7148369511232353 1112 9 0.6124069758697814 1112 10 1.0713161736670145 1112 11 0.8205615815576053 1112 12 1.0311845668730228 1112 13 0.45888309485268763 1112 14 0.658920458893796 1112 15 1.1030510765044284 1112 16 1.3036638527566353 1112 17 0.40133114047038987 1112 18 0.68290670643038 1112 19 1.5453069045780055 1112 20 1.008035671639341 1112 21 1.2078736539818067 1112 22 1.1342925067768164 1112 23 1.1723720395217327 1112 24 2.2364647050872186 1112 25 1.286313237917966 1113 1 0.04141245627585022 1113 2 0.4024466411002834 1113 3 -4.313639265021731 1113 4 -0.9136841054084037 1113 5 0.49728591477022377 1113 6 1.0659181117223966 1113 7 1.247427622259103 1113 8 1.67747449170108 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2215 5 1.4180060367473288 2215 6 1.6999055502608087 2215 7 1.1513853265191294 2215 8 -0.23441392587238852 2215 9 0.32996123400734234 2215 10 0.06410721288231258 2215 11 -0.06817169514987143 2215 12 -0.25930094657321234 2215 13 0.2808599498055031 2215 14 0.12551722048618075 2215 15 0.2501917972766563 2215 16 0.42271181582528783 2215 17 0.026335139714923628 2215 18 -0.7516909386080286 2215 19 0.6456074413540307 2215 20 -3.552558301318541 2215 21 -1.6921834191078702 2215 22 -0.8358560999895985 2215 23 -0.6190201413617729 2215 24 -0.7720090490320004 2215 25 -0.38338167390772426 2216 1 0.6605807625419011 2216 2 -0.23895352119226487 2216 3 0.5021782770771992 2216 4 0.21338736101011335 2216 5 0.6180371821183499 2216 6 -1.0109458838397032 2216 7 -8.756692681724477 2216 8 -9.741320213517742 2216 9 -4.916611907276096 2216 10 -2.5849891002099987 2216 11 -1.893606354095656 2216 12 -1.5513641835790715 2216 13 -1.3255835711886093 2216 14 -1.3151213389709753 2216 15 -2.13545534451562 2216 16 -1.2875801918658318 2216 17 -1.5427344320015095 2216 18 -1.3245539966767872 2216 19 -0.6918174415160685 2216 20 -1.5902516394327897 2216 21 -4.140372747255597 2216 22 -2.767408195830054 2216 23 -2.472236669634878 2216 24 -2.566863218767331 2216 25 -2.2205742063586698 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64714.0 3 13.0 4 497.0 5 2544.0 6 3008.0 7 2481.0 8 1978.0 9 1858.0 10 1772.0 11 1762.0 12 1737.0 13 1720.0 14 1748.0 15 1756.0 16 1913.0 17 2167.0 18 2507.0 19 3120.0 20 4003.0 21 5195.0 22 6377.0 23 7477.0 24 8658.0 25 9888.0 26 11319.0 27 12382.0 28 13900.0 29 16226.0 30 19108.0 31 23366.0 32 29753.0 33 38330.0 34 52205.0 35 71880.0 36 101982.0 37 155476.0 38 163845.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33590812026381 18.322916076119206 12.213466371865374 26.12770943175161 2 14.066014202540785 20.04321178494644 42.070484061671785 23.82028995084099 3 19.82998085986637 23.946997642294267 29.035181500860247 27.18783999697912 4 11.384428903769264 16.557304176291154 38.95804371433928 33.1002232056003 5 12.253734931807147 37.87119494246154 36.362372064737244 13.512698060994063 6 31.25695361116349 37.52310013200075 19.073048274561568 12.146897982274183 7 27.35569026433644 31.02035993240928 23.49344771763149 18.13050208562279 8 25.002126945564374 37.744775985366616 20.965538755311456 16.287558313757554 9 25.919404698348046 15.489604731111323 20.310049015274995 38.280941555265635 10 13.563088050507218 29.876796714579058 35.785179300150645 20.774935934763082 11 34.396927421046556 22.658855584546597 24.68811060907113 18.25610638533572 12 22.827393579408852 24.968179889690283 32.58744637722151 19.616980153679346 13 28.8972823909303 22.428288589813093 25.7246564687581 22.949772550498505 14 19.838236559291854 22.849940425892992 28.24331220249775 29.068510812317406 15 22.672981805282703 32.12365195399644 23.06589059128412 22.13747564943674 16 21.18693606630022 28.774879081300735 28.96674617915767 21.071438673241374 17 20.968534587911407 29.307298952645727 28.47478960400415 21.24937685543871 18 20.70158236686823 28.432163990105348 30.508202922906314 20.35805072012011 19 23.42435142537915 27.398889302702727 27.34219942579401 21.83455984612411 20 22.678868160230046 28.270067057145887 28.637408223633816 20.413656558990255 21 22.16307501573287 27.553497646551378 27.358575061694058 22.924852276021692 22 22.016414346761326 29.193448403466714 27.845345929293856 20.9447913204781 23 21.48350767693243 28.719434872176837 28.186975849600948 21.61008160128978 24 22.946951849726176 27.58744229733504 28.65917685977295 20.806428993165834 25 21.951587432531174 28.169326136658263 28.468428123600535 21.41065830721003 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 264.0 1 264.0 2 351.5 3 439.0 4 439.0 5 439.0 6 642.5 7 846.0 8 846.0 9 846.0 10 1258.5 11 1671.0 12 1671.0 13 1671.0 14 2796.0 15 3921.0 16 3921.0 17 3921.0 18 6934.0 19 9947.0 20 9947.0 21 9947.0 22 15620.0 23 21293.0 24 21293.0 25 21293.0 26 31147.0 27 41001.0 28 41001.0 29 41001.0 30 54069.5 31 67138.0 32 67138.0 33 67138.0 34 77882.0 35 88626.0 36 88626.0 37 88626.0 38 95658.5 39 102691.0 40 102691.0 41 102691.0 42 106906.0 43 111121.0 44 111121.0 45 111121.0 46 112529.5 47 113938.0 48 113938.0 49 113938.0 50 108071.0 51 102204.0 52 102204.0 53 102204.0 54 88165.5 55 74127.0 56 74127.0 57 74127.0 58 62791.5 59 51456.0 60 51456.0 61 51456.0 62 40883.5 63 30311.0 64 30311.0 65 30311.0 66 23127.0 67 15943.0 68 15943.0 69 15943.0 70 11527.0 71 7111.0 72 7111.0 73 7111.0 74 5002.0 75 2893.0 76 2893.0 77 2893.0 78 2009.5 79 1126.0 80 1126.0 81 1126.0 82 761.0 83 396.0 84 396.0 85 396.0 86 266.0 87 136.0 88 136.0 89 136.0 90 90.5 91 45.0 92 45.0 93 45.0 94 31.0 95 17.0 96 17.0 97 17.0 98 10.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23483942427223936 2 0.14269470285683986 3 0.1450513453482823 4 0.06692864675696535 5 0.022270271544131075 6 0.021798943045842588 7 0.28220793835023245 8 0.2805582886062227 9 0.018146147184106804 10 0.03664579074192997 11 0.014964679820659505 12 0.017085658062957705 13 0.014729015571515262 14 0.016732161689241336 15 0.014375519197798895 16 0.01897097205611166 17 0.017203490187529827 18 0.014846847696087384 19 0.022270271544131075 20 0.015907336817236484 21 0.014493351322371017 22 0.01637866531552497 23 0.017556986561246192 24 0.01578950469266436 25 0.014729015571515262 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 848665.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.38276791246274 #Duplication Level Percentage of deduplicated Percentage of total 1 94.44320213550856 82.52708413116949 2 4.27661708284701 7.474052760017874 3 0.6206178449339866 1.62693915318598 4 0.22588262928863312 0.7895299748234195 5 0.11209311592593714 0.48975033667704726 6 0.06573146046339759 0.3446278172532175 7 0.050393177561952225 0.3082446737487317 8 0.0278184651472136 0.194468358691192 9 0.027652653826275565 0.21747288883186014 >10 0.1257422026200567 2.0921338235599056 >50 0.012261075008598464 0.7341697499309887 >100 0.010371776357219312 1.8394652578426915 >500 8.08190255606588E-4 0.413726639824612 >1k 8.08190255606588E-4 0.9483344344429299 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1779 0.2096233496138052 No Hit GTCCTACAGTGTGCATTTCTCATTT 1444 0.1701495878821443 No Hit CTGTAGGACGTGGAATATGGCAAGA 1425 0.167910777515274 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1333 0.15707022205463875 No Hit CTGTAGGACCTGGAATATGGCGAGA 1048 0.12348806655158395 No Hit CTTTAGGACGTGAAATATGGCGAGG 1028 0.1211314240601415 No Hit GTATCAACGCAGAGTACTTTTTTTT 865 0.10192478775488562 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 1.178321245721221E-4 10 0.0 1.178321245721221E-4 0.0 0.0 1.178321245721221E-4 11 0.0 1.178321245721221E-4 0.0 0.0 1.178321245721221E-4 12 0.0 1.178321245721221E-4 0.0 0.0 1.178321245721221E-4 13 0.0 1.178321245721221E-4 0.0 0.0 1.178321245721221E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCCTT 75 1.6189006E-10 17.80318 1 CGCCTTG 75 1.6370905E-10 17.789476 2 CCAACGT 45 3.5835856E-5 16.86249 19 TCCAACG 90 2.3337634E-9 14.754679 18 CGCGTGG 40 0.0053336155 14.226888 11 CCCGATC 40 0.0053336155 14.226888 12 CCGGTTT 55 1.9856541E-4 13.795769 12 TAGGACC 400 0.0 12.610181 4 GGCGAGG 340 0.0 12.553876 19 GTCCTAC 1025 0.0 12.189284 1 GTAGGAC 1000 0.0 12.007897 3 TGTAGGA 1025 0.0 11.99395 2 TGGCGAG 835 0.0 11.927433 18 GGCGTGC 65 7.967426E-4 11.700922 8 CTGTAGG 1055 0.0 11.481062 1 AGGACGT 770 0.0 11.367068 5 AGGACCT 620 0.0 11.355111 5 CCTATAC 85 5.14751E-5 11.211856 3 GGACGTG 785 0.0 11.149863 6 CTAGTAC 60 0.005735343 11.118423 3 >>END_MODULE