FastQCFastQC Report
Thu 26 May 2016
SRR1926782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926782_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848665
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19900.234485927898523No Hit
CTGTAGGACGTGGAATATGGCAAGA16870.19878279415317No Hit
GTCCTACAGTGTGCATTTCTCATTT16040.18900272781368385No Hit
GTATCAACGCAGAGTACTTTTTTTT14990.17663035473361102No Hit
GTCCTAAAGTGTGTATTTCTCATTT14300.1684999381381346No Hit
CTTTAGGACGTGAAATATGGCGAGG13090.15424225106490783No Hit
CTGTAGGACCTGGAATATGGCGAGA12680.14941113395745081No Hit
GGTATCAACGCAGAGTACTTTTTTT9290.10946604372750143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5200.015.0146174
TAGGTTG551.9213537E-413.8494025
AGGACCT7300.013.8256875
GACGTGG3550.013.6343827
GTAGGAC10050.013.5487553
GTATTAT1153.5652192E-1013.26293851
AGGACGT7400.013.2528495
CTTACAC1153.619789E-1013.2480383
GGACGTG7200.013.2234746
ACGAAAT655.5158886E-513.13618512
TGTAGGA10900.013.1184452
GGACCTG7200.012.9590046
TAGGACG7850.012.8570084
CTGTAGG10850.012.6517431
GTATTAG853.7887366E-612.3364841
AGGTGTC550.003089292212.0789437
TCCAACG550.003094751212.07609718
CGAAATC550.00309748412.07467413
GGACCAT802.7990747E-511.9011276
GTCCTAC10800.011.8276551
Produced by FastQC (version 0.11.5)