##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926782_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 848665 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.004373928464116 31.0 30.0 33.0 26.0 34.0 2 30.338640099450313 31.0 30.0 34.0 26.0 34.0 3 30.390390790241142 31.0 30.0 34.0 26.0 34.0 4 33.997173207331514 37.0 35.0 37.0 30.0 37.0 5 33.96965704960143 37.0 35.0 37.0 30.0 37.0 6 33.95426110420484 37.0 35.0 37.0 30.0 37.0 7 33.94334984946946 37.0 35.0 37.0 30.0 37.0 8 33.97939587469732 37.0 35.0 37.0 30.0 37.0 9 35.529479830086075 39.0 35.0 39.0 30.0 39.0 10 35.425981983468155 39.0 35.0 39.0 30.0 39.0 11 35.461508369026646 39.0 35.0 39.0 30.0 39.0 12 35.35397595046337 39.0 35.0 39.0 30.0 39.0 13 35.385193215226266 39.0 35.0 39.0 30.0 39.0 14 36.50175393117426 40.0 37.0 41.0 30.0 41.0 15 36.51186628410503 40.0 37.0 41.0 30.0 41.0 16 36.49165453977718 40.0 37.0 41.0 30.0 41.0 17 36.46724443685082 40.0 37.0 41.0 30.0 41.0 18 36.43652678029611 40.0 37.0 41.0 30.0 41.0 19 36.49595305568157 40.0 37.0 41.0 30.0 41.0 20 36.46013562477538 40.0 37.0 41.0 30.0 41.0 21 36.40685193804387 40.0 37.0 41.0 30.0 41.0 22 36.32894369391927 40.0 37.0 41.0 30.0 41.0 23 36.26294945591017 40.0 37.0 41.0 30.0 41.0 24 36.206949738707266 40.0 37.0 41.0 30.0 41.0 25 36.09427041294268 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.005577994135602893 1101 2 0.0032458894617057865 1101 3 0.0036657009158247433 1101 4 0.08549624946855516 1101 5 -0.0581229396036278 1101 6 0.08120830215496966 1101 7 -0.0711917853913846 1101 8 -0.054472142498767084 1101 9 -0.09965211334444035 1101 10 0.04532169300843947 1101 11 0.2031537214996959 1101 12 0.07037849137248031 1101 13 0.16365743768375296 1101 14 0.011447073952055575 1101 15 0.004592475706857613 1101 16 0.055070878268757895 1101 17 -0.08295870683936357 1101 18 0.030004406680532725 1101 19 0.021698650135625996 1101 20 -0.10802672430356353 1101 21 0.08962397920329579 1101 22 0.08874348933066045 1101 23 -0.1639695029286301 1101 24 -0.16657235331490483 1101 25 -0.14779609706307895 1102 1 -0.39396953612315144 1102 2 -0.30708859048358406 1102 3 -0.19149898868054294 1102 4 -0.032965556426240994 1102 5 -0.1887529586743497 1102 6 -0.26882259419443955 1102 7 -0.04463932313631602 1102 8 -0.1852671112788613 1102 9 -0.29179568557147917 1102 10 -0.059385844812631206 1102 11 -0.05804160560192173 1102 12 0.08969664002769662 1102 13 0.23728020277379613 1102 14 0.2272215953124075 1102 15 0.02145985233399017 1102 16 0.0192031983879275 1102 17 -0.015635117381428643 1102 18 -0.253230739280923 1102 19 -0.040045832613806454 1102 20 0.05400400963382168 1102 21 -0.02867795235780335 1102 22 0.19138661809675028 1102 23 0.24387436328954237 1102 24 0.28395288462505164 1102 25 0.22844974905914484 1103 1 -0.6397040813271389 1103 2 -0.6510729806541491 1103 3 -0.32638996416960353 1103 4 -0.5965944032908084 1103 5 -0.7321078192069095 1103 6 -0.7867043679020256 1103 7 -0.789881519787599 1103 8 -0.4432840615992646 1103 9 -0.6172418372975201 1103 10 -0.5867400037798234 1103 11 -0.8004982119047384 1103 12 -0.6876590849336282 1103 13 -0.5713020714255066 1103 14 -0.6140367122864205 1103 15 -0.5339550421493229 1103 16 -0.5836180966265729 1103 17 -0.6641446041738348 1103 18 -0.662430015165242 1103 19 -0.7162608874549221 1103 20 -0.6009592521440226 1103 21 -0.6548425821881096 1103 22 -0.6153872460877139 1103 23 -0.5597017408929119 1103 24 -0.6381439188677405 1103 25 -0.7003557732625367 1104 1 -0.16478072720901338 1104 2 -0.1430203677763835 1104 3 0.11085726164252918 1104 4 0.09430902761469895 1104 5 -0.15302782134513393 1104 6 0.032318275256756124 1104 7 -0.06647520385248384 1104 8 0.07725137473984489 1104 9 -0.08085776203204631 1104 10 0.17517131654179252 1104 11 0.058118042996667896 1104 12 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2216 24 -0.760708808976247 2216 25 -0.5948073507935874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18431.0 3 13.0 4 301.0 5 2023.0 6 2672.0 7 2046.0 8 1517.0 9 1364.0 10 1185.0 11 1139.0 12 1006.0 13 968.0 14 1006.0 15 1008.0 16 1055.0 17 1080.0 18 1138.0 19 1450.0 20 1811.0 21 2517.0 22 3042.0 23 3757.0 24 4389.0 25 5390.0 26 6476.0 27 7154.0 28 8121.0 29 10220.0 30 12906.0 31 16833.0 32 21841.0 33 28812.0 34 39495.0 35 55500.0 36 81436.0 37 143606.0 38 355957.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.048651703021946 19.206345648632357 10.187696340328348 28.55730630801735 2 14.016700367842235 20.95509210212754 40.44141068323333 24.586796846796894 3 18.590539089688786 24.49550571993924 29.241399467073965 27.672555723298014 4 11.72488555554901 17.290568127588624 38.595441075100304 32.38910524176206 5 12.505553166041139 38.780129976491104 36.083029406739215 12.63128745072854 6 31.514533099854685 38.718949487974704 17.69984120771993 12.066676204450676 7 28.112057891077093 31.7032610873434 21.823238930335066 18.361442091244445 8 24.419558367056414 39.12651518186896 19.9241554695031 16.529770981571524 9 25.996785210080866 15.600805111610248 19.78451008628553 38.61789959202336 10 13.623572997483008 30.47575391924886 35.1192271797434 20.78144590352473 11 34.61709779596146 22.69815607383246 24.514626029902526 18.17012010030355 12 22.94319141891247 25.248834334719444 32.42051853693542 19.38745570943267 13 29.1628665493049 22.369435037435576 25.65144027393838 22.816258139321143 14 19.872293392909327 22.99508403797027 27.860260362169242 29.272362206951165 15 22.13951482428966 32.535312340553894 23.07324298496066 22.251929850195783 16 21.142631575846487 29.10805922312365 28.641085010987975 21.10822419004189 17 20.91428409830039 29.612426119170838 28.304370162985588 21.16891961954319 18 20.651452824875065 28.499381964680666 30.384713730046414 20.46445148039785 19 22.787335328635734 27.626847029434636 27.68269978554427 21.90311785638536 20 22.715800041477348 28.248901792952623 28.674398107124677 20.360900058445353 21 21.9957008204739 27.48799694531981 27.54338659381946 22.97291564038683 22 22.023884571650772 29.227315843118305 27.614547554099673 21.134252031131247 23 21.326772409713655 28.931693573759134 28.074460827114713 21.6670731894125 24 22.224081351298803 27.770557287033164 28.977158242651697 21.028203119016336 25 21.76298541372557 28.37488775709811 28.534561386556305 21.327565442620013 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 250.0 1 250.0 2 271.5 3 293.0 4 293.0 5 293.0 6 467.0 7 641.0 8 641.0 9 641.0 10 1112.0 11 1583.0 12 1583.0 13 1583.0 14 2647.5 15 3712.0 16 3712.0 17 3712.0 18 6565.5 19 9419.0 20 9419.0 21 9419.0 22 15391.0 23 21363.0 24 21363.0 25 21363.0 26 30955.5 27 40548.0 28 40548.0 29 40548.0 30 54192.5 31 67837.0 32 67837.0 33 67837.0 34 77946.0 35 88055.0 36 88055.0 37 88055.0 38 95139.5 39 102224.0 40 102224.0 41 102224.0 42 106866.5 43 111509.0 44 111509.0 45 111509.0 46 113570.5 47 115632.0 48 115632.0 49 115632.0 50 109282.0 51 102932.0 52 102932.0 53 102932.0 54 89033.5 55 75135.0 56 75135.0 57 75135.0 58 63064.5 59 50994.0 60 50994.0 61 50994.0 62 40668.5 63 30343.0 64 30343.0 65 30343.0 66 23005.5 67 15668.0 68 15668.0 69 15668.0 70 11190.0 71 6712.0 72 6712.0 73 6712.0 74 4625.0 75 2538.0 76 2538.0 77 2538.0 78 1717.5 79 897.0 80 897.0 81 897.0 82 590.0 83 283.0 84 283.0 85 283.0 86 178.0 87 73.0 88 73.0 89 73.0 90 46.0 91 19.0 92 19.0 93 19.0 94 12.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11382583233666993 2 0.12007093493899242 3 0.016732161689241336 4 0.0 5 0.005891606228606105 6 0.3424201540065868 7 0.020620621800121367 8 0.010251394837774623 9 0.010251394837774623 10 0.003888460110880029 11 0.004831117107457006 12 9.426569965769767E-4 13 8.248248720048548E-4 14 1.1783212457212209E-4 15 0.0028279709897309304 16 0.0011783212457212211 17 1.1783212457212209E-4 18 9.426569965769767E-4 19 5.891606228606106E-4 20 0.001060489121149099 21 0.015436008318947994 22 0.0 23 4.7132849828848835E-4 24 0.0 25 0.006952095349755203 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 848665.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.8133829300924 #Duplication Level Percentage of deduplicated Percentage of total 1 92.19654443624984 77.27304283666689 2 6.077614113155678 10.187707979345017 3 0.8858793788868755 2.227456428375543 4 0.30321613703168643 1.0165428081448042 5 0.14231889355984662 0.5964113962059242 6 0.07626812663922396 0.3835373822024436 7 0.0547225791323434 0.3210539135818967 8 0.03949900283588642 0.2648436040032764 9 0.03314721412810406 0.25003621357060996 >10 0.16051795268011385 2.4590848644897823 >50 0.015912272991518855 0.9223089958968617 >100 0.011966577441772661 2.0324675092435083 >500 0.001407832510109751 0.7926917890123364 >1k 9.854827570768257E-4 1.2728142792611004 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1990 0.234485927898523 No Hit CTGTAGGACGTGGAATATGGCAAGA 1687 0.19878279415317 No Hit GTCCTACAGTGTGCATTTCTCATTT 1604 0.18900272781368385 No Hit GTATCAACGCAGAGTACTTTTTTTT 1499 0.17663035473361102 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1430 0.1684999381381346 No Hit CTTTAGGACGTGAAATATGGCGAGG 1309 0.15424225106490783 No Hit CTGTAGGACCTGGAATATGGCGAGA 1268 0.14941113395745081 No Hit GGTATCAACGCAGAGTACTTTTTTT 929 0.10946604372750143 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.178321245721221E-4 0.0 4 0.0 0.0 0.0 1.178321245721221E-4 0.0 5 0.0 0.0 0.0 1.178321245721221E-4 0.0 6 0.0 0.0 0.0 1.178321245721221E-4 1.178321245721221E-4 7 0.0 0.0 0.0 2.356642491442442E-4 1.178321245721221E-4 8 0.0 0.0 0.0 2.356642491442442E-4 1.178321245721221E-4 9 0.0 0.0 0.0 4.713284982884884E-4 2.356642491442442E-4 10 0.0 0.0 0.0 4.713284982884884E-4 2.356642491442442E-4 11 0.0 0.0 0.0 4.713284982884884E-4 2.356642491442442E-4 12 0.0 0.0 0.0 4.713284982884884E-4 3.534963737163663E-4 13 0.0 0.0 0.0 4.713284982884884E-4 3.534963737163663E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 520 0.0 15.014617 4 TAGGTTG 55 1.9213537E-4 13.849402 5 AGGACCT 730 0.0 13.825687 5 GACGTGG 355 0.0 13.634382 7 GTAGGAC 1005 0.0 13.548755 3 GTATTAT 115 3.5652192E-10 13.2629385 1 AGGACGT 740 0.0 13.252849 5 CTTACAC 115 3.619789E-10 13.248038 3 GGACGTG 720 0.0 13.223474 6 ACGAAAT 65 5.5158886E-5 13.136185 12 TGTAGGA 1090 0.0 13.118445 2 GGACCTG 720 0.0 12.959004 6 TAGGACG 785 0.0 12.857008 4 CTGTAGG 1085 0.0 12.651743 1 GTATTAG 85 3.7887366E-6 12.336484 1 AGGTGTC 55 0.0030892922 12.078943 7 TCCAACG 55 0.0030947512 12.076097 18 CGAAATC 55 0.003097484 12.074674 13 GGACCAT 80 2.7990747E-5 11.901127 6 GTCCTAC 1080 0.0 11.827655 1 >>END_MODULE