FastQCFastQC Report
Thu 26 May 2016
SRR1926781_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926781_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666042
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9880.14833899363703792No Hit
GTCCTACAGTGGACATTTCTAAATT9380.1408319595460947No Hit
GTCCTAAAGTGTGTATTTCTCATTT8710.13077253386423077No Hit
CTGTAGGACGTGGAATATGGCAAGA8040.12071310818236687No Hit
GTCCTACAGTGTGCATTTCTCATTT7370.11065368250050298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1400.014.2891044
CGCCTTG551.8942117E-413.8718012
GACTAAG603.9300058E-412.72543051
TCCTAGA1051.881017E-812.7158182
AAGCATC604.0160763E-412.6928066
TAAACTG701.0675717E-412.2404155
GGCGAGG1551.8189894E-1212.23263119
TTTATAC959.893865E-712.0465643
GTCCTAA3600.011.9300921
TTATACC657.8306545E-411.7243944
CTGATTG658.1382884E-411.6706769
TCTAGAC907.128301E-611.6561663
GTATTAA1156.606933E-811.6188721
GTCTAGA751.983255E-411.4528881
TTAGGGT752.0183605E-411.4312844
TAAAGTG3750.011.4243875
TGTAGGA5450.011.3742422
GTATAGA1354.3746695E-911.3114941
TAGGACT855.1657185E-511.2071414
CAATACT600.005751111511.1137494
Produced by FastQC (version 0.11.5)