##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926781_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 666042 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.71740070446008 30.0 27.0 31.0 10.0 33.0 2 27.33470261635152 31.0 28.0 31.0 10.0 34.0 3 26.96313295557938 31.0 27.0 31.0 10.0 34.0 4 30.652451346912056 35.0 32.0 37.0 10.0 37.0 5 30.28979703982632 35.0 30.0 37.0 10.0 37.0 6 30.054886929052522 35.0 30.0 37.0 10.0 37.0 7 30.085832424982208 35.0 30.0 37.0 10.0 37.0 8 29.796410736860437 35.0 30.0 37.0 8.0 37.0 9 31.952267274436146 37.0 32.0 39.0 10.0 39.0 10 32.10726951153231 37.0 32.0 39.0 8.0 39.0 11 32.31917056281736 37.0 32.0 39.0 8.0 39.0 12 32.15042594911432 37.0 32.0 39.0 2.0 39.0 13 32.29668399290135 37.0 32.0 39.0 2.0 39.0 14 33.23300782833515 38.0 33.0 40.0 2.0 41.0 15 32.77685791586717 38.0 32.0 40.0 2.0 41.0 16 32.86765098897667 38.0 32.0 40.0 2.0 41.0 17 33.09732118995499 38.0 33.0 40.0 2.0 41.0 18 33.149047657655224 38.0 33.0 40.0 2.0 41.0 19 32.53535813056834 38.0 32.0 40.0 2.0 41.0 20 32.79407454785134 39.0 32.0 40.0 2.0 41.0 21 32.76681200284667 39.0 32.0 40.0 2.0 41.0 22 32.787761132180854 39.0 32.0 40.0 2.0 41.0 23 32.77485654057852 39.0 33.0 40.0 2.0 41.0 24 31.479190501499904 38.0 30.0 40.0 2.0 41.0 25 32.03663132355017 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.7055721877344361 1101 2 -0.7264219240213343 1101 3 -1.5409855135803063 1101 4 -0.8406038250495378 1101 5 -1.0101983461388677 1101 6 -2.8000398351787403 1101 7 -1.1060796404324122 1101 8 -0.4741293635573669 1101 9 -0.8114963262316408 1101 10 -8.569133419526413 1101 11 -4.220771419590591 1101 12 -1.9053582181523865 1101 13 -1.091037326209932 1101 14 -0.7992315526717277 1101 15 -1.996678502394385 1101 16 -0.6836109227048013 1101 17 -0.8083664969353279 1101 18 -0.5378059552055703 1101 19 -1.27210659857559 1101 20 -0.3576443099030371 1101 21 -0.7315989229498641 1101 22 -0.27635893164826797 1101 23 -0.705963228000769 1101 24 -0.5375708779302641 1101 25 -0.4049135671033852 1102 1 -1.1116935258722371 1102 2 -1.0446041380667737 1102 3 -1.4871954657896715 1102 4 -1.2563573168140891 1102 5 -1.3274650265947834 1102 6 -1.7252926683251921 1102 7 -0.9341073785963374 1102 8 -0.4828122362209051 1102 9 -0.6381600173234254 1102 10 -0.6910561489269078 1102 11 -1.139124547754406 1102 12 -0.8805963548619076 1102 13 -1.0573977263884657 1102 14 -1.4715793901182472 1102 15 -0.7029522480262784 1102 16 -0.6924853933609114 1102 17 -1.1561897913321744 1102 18 -1.2574099134459154 1102 19 -0.9930321475147252 1102 20 -0.7710256878845101 1102 21 -1.0001485479185632 1102 22 -0.879998204882245 1102 23 -1.2662393032900745 1102 24 -4.606905401493552 1102 25 -2.6091058618159337 1103 1 -0.820811058643411 1103 2 -0.8971508607296528 1103 3 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0.5965349501159913 2215 4 0.8100443763574816 2215 5 1.2876203344504873 2215 6 1.6024910262875665 2215 7 1.064912793530688 2215 8 -0.3049775561152188 2215 9 0.32568786915794234 2215 10 0.15980335318275962 2215 11 -0.13763350533901075 2215 12 -0.22439719872846453 2215 13 0.2860831551846985 2215 14 0.08429572488236659 2215 15 -0.003058751055945663 2215 16 0.14862658890972824 2215 17 -0.12556042640747478 2215 18 -0.8324256622981778 2215 19 0.2727232762087084 2215 20 -3.1544320626573388 2215 21 -1.8159535975217835 2215 22 -0.7748457242680615 2215 23 -0.7192723736137978 2215 24 -0.6490969166011205 2215 25 -0.24185441042889977 2216 1 0.6110750029655705 2216 2 -0.5555242117982182 2216 3 0.5397081770720966 2216 4 0.38973973100229387 2216 5 0.7368097125833373 2216 6 -0.8937356811714494 2216 7 -8.924411285848635 2216 8 -10.419326590179715 2216 9 -5.050682836744336 2216 10 -2.502881374549915 2216 11 -1.5237914928202265 2216 12 -1.1068453164064742 2216 13 -0.804114788621316 2216 14 -0.8575434624417397 2216 15 -2.0572080755677575 2216 16 -0.9462213947156712 2216 17 -1.0704446258575686 2216 18 -0.8439880598218039 2216 19 -0.2514317779708861 2216 20 -1.1335962518733105 2216 21 -3.6679350175424688 2216 22 -2.360071829931787 2216 23 -2.249405020828423 2216 24 -2.4544907321634746 2216 25 -1.7504838666870306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53373.0 3 12.0 4 421.0 5 2121.0 6 2601.0 7 2167.0 8 1855.0 9 1633.0 10 1500.0 11 1451.0 12 1433.0 13 1386.0 14 1397.0 15 1465.0 16 1627.0 17 1749.0 18 2033.0 19 2447.0 20 3152.0 21 4028.0 22 4864.0 23 5884.0 24 6739.0 25 7692.0 26 8782.0 27 9616.0 28 10732.0 29 12562.0 30 14934.0 31 18097.0 32 22884.0 33 29800.0 34 39863.0 35 54918.0 36 78794.0 37 119897.0 38 132133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.196518273945145 18.717738804330487 12.240066937748496 25.845675983975873 2 13.96026772827865 20.79058621187338 41.97175983835274 23.277386221495235 3 19.265522622781248 25.456830735830504 29.47140842486987 25.806238216518373 4 11.27508207312584 17.186901354488292 39.51890499049679 32.01911158188907 5 11.953354958851445 38.36141647143629 36.425331891632126 13.259896678080136 6 29.968809083659835 38.26331020169725 19.771767189919192 11.99611352472372 7 26.356090603344533 31.546549509073742 24.515135951707133 17.582223935874595 8 24.73934762735935 37.36481693717084 21.682301542001817 16.21353389346799 9 26.139483057923226 15.547756774127668 20.601997849578627 37.710762318370485 10 13.481667725554338 30.295752583761022 35.813083045330906 20.409496645353737 11 33.74978789348574 23.268347401188397 24.905434281518275 18.076430423807587 12 22.127533689929514 25.806170655598372 32.89915095671291 19.167144697759213 13 28.492378476836606 22.799699073344115 26.10839065805932 22.599531791759954 14 19.822112901094414 22.994978436107207 28.693492389567645 28.489416273230738 15 22.55212885128824 31.924210071086843 23.57548509939244 21.94817597823247 16 21.077646663082618 29.2093273305562 28.708361740404918 21.004664265956265 17 20.79435848997103 29.335161378001295 28.603242726990135 21.267237405037534 18 20.491212474322165 28.911590163737493 30.202990041204664 20.394207320735678 19 22.95580010932835 27.619224970114914 28.084020640231632 21.340954280325104 20 22.501681863504764 28.674271092370347 28.647691642339712 20.17635540178518 21 22.298520909978226 27.73526541031609 27.723702980704257 22.24251069900143 22 21.797374530361687 29.096702696046776 28.250227498656034 20.855695274935503 23 21.410561945221566 28.65874077778228 28.42763158882687 21.503065688169283 24 22.554235610876102 27.995290845830763 28.744010319294468 20.706463223998668 25 21.764827778828913 28.157838623552063 28.656527706450674 21.420805891168353 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 295.0 1 295.0 2 392.0 3 489.0 4 489.0 5 489.0 6 705.5 7 922.0 8 922.0 9 922.0 10 1338.5 11 1755.0 12 1755.0 13 1755.0 14 2824.0 15 3893.0 16 3893.0 17 3893.0 18 6534.0 19 9175.0 20 9175.0 21 9175.0 22 14130.5 23 19086.0 24 19086.0 25 19086.0 26 27131.0 27 35176.0 28 35176.0 29 35176.0 30 45778.0 31 56380.0 32 56380.0 33 56380.0 34 64235.5 35 72091.0 36 72091.0 37 72091.0 38 77104.5 39 82118.0 40 82118.0 41 82118.0 42 84079.5 43 86041.0 44 86041.0 45 86041.0 46 86188.0 47 86335.0 48 86335.0 49 86335.0 50 81126.5 51 75918.0 52 75918.0 53 75918.0 54 65825.5 55 55733.0 56 55733.0 57 55733.0 58 46662.5 59 37592.0 60 37592.0 61 37592.0 62 29926.0 63 22260.0 64 22260.0 65 22260.0 66 16980.5 67 11701.0 68 11701.0 69 11701.0 70 8594.5 71 5488.0 72 5488.0 73 5488.0 74 3876.0 75 2264.0 76 2264.0 77 2264.0 78 1572.5 79 881.0 80 881.0 81 881.0 82 577.0 83 273.0 84 273.0 85 273.0 86 198.0 87 123.0 88 123.0 89 123.0 90 80.5 91 38.0 92 38.0 93 38.0 94 23.5 95 9.0 96 9.0 97 9.0 98 7.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23181721272832645 2 0.1336252068187892 3 0.13752886454607968 4 0.07161710522759826 5 0.02432279045465601 6 0.021470117500097592 7 0.3142444470468829 8 0.3055362875013888 9 0.0189177259091769 10 0.037385029772897205 11 0.01546449022734302 12 0.017416319090988258 13 0.014863927500067563 14 0.017116037727350528 15 0.01441350545461097 16 0.019218007272814627 17 0.018167022500082577 18 0.0147137868182487 19 0.023722227727380554 20 0.017116037727350528 21 0.013812942727335515 22 0.015614630909161886 23 0.018467303863720304 24 0.01576477159098075 25 0.01441350545461097 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 666042.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.24473065374292 #Duplication Level Percentage of deduplicated Percentage of total 1 94.35338221355937 83.26188799705204 2 4.367269638305663 7.707770658491049 3 0.6601879862950479 1.7477433309433028 4 0.21725764434636488 0.7668736923124658 5 0.10632254108504992 0.46912020002358723 6 0.06407826495501624 0.3392741539028749 7 0.04542364737110767 0.2805878269301796 8 0.028752250045867782 0.2029787648789336 9 0.024296145907683368 0.19296061663928016 >10 0.11229837383442531 1.920210321819205 >50 0.00917633410186274 0.5428376864141865 >100 0.010195553114331811 1.6641521979158014 >500 0.0013594070783057904 0.903602552677047 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 988 0.14833899363703792 No Hit GTCCTACAGTGGACATTTCTAAATT 938 0.1408319595460947 No Hit GTCCTAAAGTGTGTATTTCTCATTT 871 0.13077253386423077 No Hit CTGTAGGACGTGGAATATGGCAAGA 804 0.12071310818236687 No Hit GTCCTACAGTGTGCATTTCTCATTT 737 0.11065368250050298 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.5014068181886428E-4 0.0 0.0 0.0 0.0 3 1.5014068181886428E-4 0.0 0.0 0.0 0.0 4 1.5014068181886428E-4 0.0 0.0 0.0 0.0 5 1.5014068181886428E-4 0.0 0.0 0.0 0.0 6 1.5014068181886428E-4 0.0 0.0 0.0 0.0 7 1.5014068181886428E-4 0.0 0.0 0.0 0.0 8 1.5014068181886428E-4 0.0 0.0 0.0 0.0 9 1.5014068181886428E-4 0.0 0.0 0.0 0.0 10 1.5014068181886428E-4 0.0 0.0 0.0 0.0 11 1.5014068181886428E-4 0.0 0.0 0.0 0.0 12 1.5014068181886428E-4 0.0 0.0 0.0 0.0 13 1.5014068181886428E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 140 0.0 14.289104 4 CGCCTTG 55 1.8942117E-4 13.871801 2 GACTAAG 60 3.9300058E-4 12.7254305 1 TCCTAGA 105 1.881017E-8 12.715818 2 AAGCATC 60 4.0160763E-4 12.692806 6 TAAACTG 70 1.0675717E-4 12.240415 5 GGCGAGG 155 1.8189894E-12 12.232631 19 TTTATAC 95 9.893865E-7 12.046564 3 GTCCTAA 360 0.0 11.930092 1 TTATACC 65 7.8306545E-4 11.724394 4 CTGATTG 65 8.1382884E-4 11.670676 9 TCTAGAC 90 7.128301E-6 11.656166 3 GTATTAA 115 6.606933E-8 11.618872 1 GTCTAGA 75 1.983255E-4 11.452888 1 TTAGGGT 75 2.0183605E-4 11.431284 4 TAAAGTG 375 0.0 11.424387 5 TGTAGGA 545 0.0 11.374242 2 GTATAGA 135 4.3746695E-9 11.311494 1 TAGGACT 85 5.1657185E-5 11.207141 4 CAATACT 60 0.0057511115 11.113749 4 >>END_MODULE