FastQCFastQC Report
Thu 26 May 2016
SRR1926781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926781_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666042
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17620.26454788136483887No Hit
GGTATCAACGCAGAGTACTTTTTTT10890.16350320250074318No Hit
GTCCTACAGTGGACATTTCTAAATT10260.15404433954615473No Hit
TATCAACGCAGAGTACTTTTTTTTT9930.14908969704613223No Hit
CTGTAGGACGTGGAATATGGCAAGA8680.1303221118187742No Hit
GTCCTAAAGTGTGTATTTCTCATTT8330.12506718795511396No Hit
GTCCTACAGTGTGCATTTCTCATTT7750.11635902840961981No Hit
CTTTAGGACGTGAAATATGGCGAGG7490.11245537068232934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGT5200.012.8171175
GGACGTG4950.012.3103516
TAGGACG5650.012.301844
CTAACAC701.0665649E-412.2416543
GACGTGG2500.012.14423757
TAGGACC2750.012.1180024
ACGTGGA2650.011.8139678
GATATAC2750.011.7895411
CTGTAGG6500.011.7361951
GGTATAT657.7648327E-411.7361941
GGACCTG4150.011.7008596
GGTATCA4200.011.5790131
GTAGGAC6050.011.4884953
TGTAGGA6750.011.442792
CTCCTAT1001.8391074E-611.442791
TAAAGGT752.0278059E-411.4255454
ATGCCGG752.092593E-411.38693319
CGTGGAA2600.011.3122419
TCCTATA1354.434696E-911.301522
TATACTG1354.5401976E-911.2844895
Produced by FastQC (version 0.11.5)