FastQCFastQC Report
Thu 26 May 2016
SRR1926780_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926780_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596663
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13420.2249175832924113No Hit
GTCCTAAAGTGTGTATTTCTCATTT11370.19055983025594012No Hit
CTGTAGGACGTGGAATATGGCAAGA11090.18586706398754405No Hit
GTCCTACAGTGTGCATTTCTCATTT9630.16139763987376457No Hit
CTTTAGGACGTGAAATATGGCGAGG8350.13994499407538258No Hit
GTATCAACGCAGAGTACTTTTTTTT7000.11731915670990156No Hit
CTGTAGGACCTGGAATATGGCGAGA6590.11044760610260734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT902.224624E-914.8021044
TTAGTAC400.005167511314.2938883
ATAGACC707.0179885E-613.6132263
GTCCTAA4000.013.1148771
TCCAACG604.136129E-412.64817118
CTAAAAC550.002994830512.1281473
AACACCT550.003036643612.1057237
TATACTC802.8237919E-511.889555
CTAGGAC1800.011.6468713
ACTGTTC1800.011.6175268
TAGGACC3700.011.5730744
TCCTACA8400.011.4577992
TAAAGTG4000.011.4139685
TCCTAAA4350.011.3912972
CTAAAGT4100.011.3723484
GTCCTAC7750.011.32263851
TATACTG1354.609319E-911.2730545
GGACCAT600.00581519611.0969126
CCTACAC951.3107197E-511.0338783
GGACCTG5150.010.896836
Produced by FastQC (version 0.11.5)