##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926780_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 596663 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.466492810849676 30.0 26.0 31.0 10.0 31.0 2 27.134709207710216 31.0 28.0 31.0 10.0 34.0 3 26.7164563581117 31.0 27.0 31.0 10.0 34.0 4 30.44357870355628 35.0 30.0 37.0 10.0 37.0 5 29.949993547446383 35.0 30.0 37.0 10.0 37.0 6 29.819826267088793 35.0 30.0 37.0 10.0 37.0 7 29.918459834110713 35.0 30.0 37.0 10.0 37.0 8 29.6141741653161 35.0 29.0 37.0 8.0 37.0 9 31.828626879829987 37.0 32.0 39.0 10.0 39.0 10 32.01255482575591 37.0 32.0 39.0 8.0 39.0 11 32.23339808233458 37.0 32.0 39.0 8.0 39.0 12 32.10277158127787 37.0 32.0 39.0 2.0 39.0 13 32.2250784781359 37.0 32.0 39.0 2.0 39.0 14 33.13937851014727 38.0 33.0 40.0 2.0 41.0 15 32.73273355311122 38.0 32.0 40.0 2.0 41.0 16 32.79137469559869 38.0 32.0 40.0 2.0 41.0 17 33.013426339491474 38.0 32.0 40.0 2.0 41.0 18 33.06396072825029 38.0 33.0 40.0 2.0 41.0 19 32.42745569944843 38.0 32.0 40.0 2.0 41.0 20 32.71802843481161 39.0 32.0 40.0 2.0 41.0 21 32.71542394953265 39.0 32.0 40.0 2.0 41.0 22 32.71606920489455 39.0 32.0 40.0 2.0 41.0 23 32.682993582642126 39.0 32.0 40.0 2.0 41.0 24 31.3574597385794 38.0 30.0 40.0 2.0 41.0 25 31.913446954143293 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.7836079081499143 1101 2 -0.8474230985650273 1101 3 -1.807927455348949 1101 4 -1.320471341914324 1101 5 -1.2278460214429892 1101 6 -2.7413882740677096 1101 7 -1.1640089558691322 1101 8 -0.7464439119903297 1101 9 -0.8086014905024967 1101 10 -9.532974438162505 1101 11 -4.694024443328704 1101 12 -2.212361080809792 1101 13 -1.5323856001928888 1101 14 -1.2644624053403994 1101 15 -2.8614237687088355 1101 16 -1.142557500348758 1101 17 -1.0611813470876896 1101 18 -0.9177750028759561 1101 19 -1.390807058915712 1101 20 -0.7614869019962143 1101 21 -1.1047430039633532 1101 22 -1.0978412100661536 1101 23 -1.120601519014393 1101 24 -0.9000166562622667 1101 25 -1.164512535392543 1102 1 -0.6148844336615618 1102 2 -0.33948430765679305 1102 3 -0.5833424322422793 1102 4 -0.17119519365439828 1102 5 -0.46051366267660043 1102 6 -1.1321715429538735 1102 7 -0.1863045325927004 1102 8 0.2635178912728975 1102 9 0.21187384666622577 1102 10 0.18606734246561984 1102 11 0.22989095797281323 1102 12 0.07295203811881379 1102 13 -0.06657875307414685 1102 14 -0.21119917795192578 1102 15 0.3567220390814114 1102 16 0.33092877711987967 1102 17 0.17844423057901082 1102 18 -0.03867946387755694 1102 19 0.3041684197224086 1102 20 0.2394750898928777 1102 21 0.3656191576890109 1102 22 0.16418840419396474 1102 23 -0.16537658267561994 1102 24 -3.4478757527443946 1102 25 -1.8235760079946353 1103 1 -1.1038463450370806 1103 2 -1.019323658186206 1103 3 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7 0.8514927500183695 2212 8 0.8801526237458255 2212 9 0.7925007752660242 2212 10 0.8285862301954694 2212 11 0.6243357176370878 2212 12 0.5423388579532187 2212 13 0.5104059316066056 2212 14 0.3663441732616448 2212 15 -0.3776953110061001 2212 16 0.5430671169200707 2212 17 0.7794493938798581 2212 18 0.740640708785314 2212 19 1.4546592170006534 2212 20 0.11559296853130263 2212 21 -0.09877239771718394 2212 22 0.5941892334645971 2212 23 0.919180652274207 2212 24 1.7401591813722384 2212 25 1.2376088888048145 2213 1 0.21626826136026978 2213 2 -0.05156454050228376 2213 3 0.46416482129754044 2213 4 -6.837500327421569 2213 5 -1.306194030982521 2213 6 -3.2585089858637097 2213 7 -1.3294006271221122 2213 8 -2.9589049283176116 2213 9 -0.8061799164793833 2213 10 -0.10745618463104023 2213 11 -0.20467845176752064 2213 12 0.260024719960704 2213 13 0.08009834770658131 2213 14 -0.16053652018171505 2213 15 -2.755067136898159 2213 16 -1.1966072159972647 2213 17 -0.41552000054576865 2213 18 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1.5470145963349893 2215 6 1.9854978821303035 2215 7 1.4651758829420736 2215 8 0.29010457953432933 2215 9 0.3342517984683262 2215 10 0.3765170415371486 2215 11 0.33593936225557997 2215 12 0.02898260877250891 2215 13 0.32091011492783394 2215 14 0.5241149967548324 2215 15 0.45612040646314256 2215 16 0.5151748408229508 2215 17 0.3064167824928177 2215 18 -0.40809243464611455 2215 19 0.7927851806418644 2215 20 -3.0620624156871408 2215 21 -1.7543428421001295 2215 22 -0.6387971023371861 2215 23 -0.29344671482393636 2215 24 -0.01838588426460319 2215 25 -0.005182918918460899 2216 1 0.6363753694420602 2216 2 -0.05174305397193635 2216 3 0.7808964019531714 2216 4 0.6791663392450964 2216 5 0.8610893185793067 2216 6 -1.0232444613586793 2216 7 -9.089705722870246 2216 8 -10.42841158200148 2216 9 -5.4169069531557845 2216 10 -3.073944337828056 2216 11 -1.9241526484569071 2216 12 -1.3125004342100155 2216 13 -1.060813693838373 2216 14 -1.175499194638057 2216 15 -2.0769756081737185 2216 16 -1.1948707667924623 2216 17 -1.339943888893707 2216 18 -1.2162699861535486 2216 19 -0.5724960221264155 2216 20 -1.5333932565112605 2216 21 -4.466504430942173 2216 22 -3.0432146771787494 2216 23 -2.696521251455863 2216 24 -3.032290903753907 2216 25 -2.5363631485475047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48701.0 3 12.0 4 320.0 5 1847.0 6 2216.0 7 1882.0 8 1450.0 9 1383.0 10 1273.0 11 1237.0 12 1253.0 13 1223.0 14 1266.0 15 1270.0 16 1466.0 17 1549.0 18 1720.0 19 2299.0 20 2961.0 21 3627.0 22 4560.0 23 5208.0 24 6259.0 25 7172.0 26 8041.0 27 8970.0 28 9936.0 29 11901.0 30 14053.0 31 16787.0 32 21201.0 33 28194.0 34 37391.0 35 51386.0 36 72665.0 37 109468.0 38 104516.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55195849031883 18.943915233710324 12.508167725181455 25.995958550789393 2 13.465873328913469 20.877986555778413 43.034768670431944 22.621371444876175 3 19.195191424864337 25.452818122460215 29.64924324696317 25.702747205712278 4 11.034481601929453 17.40214782525565 40.064002039477984 31.499368533336913 5 11.532407182336186 38.455941889529086 37.012735554106406 12.998915374028321 6 29.53457339806346 38.666081055709036 20.1098691092455 11.689476436982002 7 25.981160508636027 31.36723232180687 25.010121984614585 17.641485184942518 8 24.133830095309765 38.51277123976604 21.792912071114458 15.560486593809737 9 25.869632634587497 15.539146250492418 21.11341139394346 37.47780972097662 10 13.16025967754916 30.513956326957643 36.62630442363924 19.69947957185396 11 33.26320871266283 23.07591730547123 25.74121165286804 17.919662328997898 12 22.23594925505237 25.65409218307876 33.65859137174965 18.45136719011922 13 28.313690255681294 23.145920883460757 26.437274856134223 22.103114004723725 14 19.244057108085954 23.643163306529345 29.009157610472165 28.103621974912542 15 22.01486845523958 33.15095588913194 23.57329042099352 21.26088523463495 16 20.28373526088691 29.384854712660633 30.11254865541739 20.218861371035064 17 20.31568501233773 29.900728194399743 29.420964757000323 20.362622036262206 18 19.879577650121444 29.09161921460624 31.503083475954924 19.525719659317396 19 22.864356146838823 27.8108958992017 28.160927247794703 21.163820706164778 20 22.12929949224964 28.936071061582126 29.42454400531051 19.51008544085773 21 21.54754940333809 28.11661350153964 27.972958702178623 22.36287839294365 22 21.487038076958537 29.958511788519083 28.57221882307583 19.982231311446554 23 20.77240671795035 29.35507609575711 29.025347371808945 20.84716981448359 24 22.450902166406287 28.193119262979327 29.387123594450888 19.9688549761635 25 21.233897395925002 28.914703343307103 29.21492209566435 20.63647716510355 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 302.0 1 302.0 2 372.0 3 442.0 4 442.0 5 442.0 6 631.0 7 820.0 8 820.0 9 820.0 10 1165.5 11 1511.0 12 1511.0 13 1511.0 14 2487.5 15 3464.0 16 3464.0 17 3464.0 18 5996.0 19 8528.0 20 8528.0 21 8528.0 22 13392.5 23 18257.0 24 18257.0 25 18257.0 26 26172.5 27 34088.0 28 34088.0 29 34088.0 30 44810.5 31 55533.0 32 55533.0 33 55533.0 34 62577.0 35 69621.0 36 69621.0 37 69621.0 38 73425.5 39 77230.0 40 77230.0 41 77230.0 42 78356.0 43 79482.0 44 79482.0 45 79482.0 46 78449.5 47 77417.0 48 77417.0 49 77417.0 50 71415.5 51 65414.0 52 65414.0 53 65414.0 54 55233.5 55 45053.0 56 45053.0 57 45053.0 58 36836.0 59 28619.0 60 28619.0 61 28619.0 62 22504.5 63 16390.0 64 16390.0 65 16390.0 66 12395.5 67 8401.0 68 8401.0 69 8401.0 70 6059.5 71 3718.0 72 3718.0 73 3718.0 74 2602.5 75 1487.0 76 1487.0 77 1487.0 78 1024.5 79 562.0 80 562.0 81 562.0 82 378.5 83 195.0 84 195.0 85 195.0 86 144.5 87 94.0 88 94.0 89 94.0 90 60.5 91 27.0 92 27.0 93 27.0 94 16.5 95 6.0 96 6.0 97 6.0 98 4.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22324159533941268 2 0.14547575432027796 3 0.14983332299807428 4 0.07240267956953925 5 0.02413422652317975 6 0.022625837365481016 7 0.2386606845069998 8 0.22474998449711145 9 0.01977665784538341 10 0.03871532171426752 11 0.017765472301785094 12 0.01860346627828439 13 0.01675987952998594 14 0.018435867482984532 15 0.016424681939386218 16 0.020279454231282985 17 0.019944256640683264 18 0.0160894843487865 19 0.02430182531847961 20 0.018435867482984532 21 0.015754286758186783 22 0.01810066989238481 23 0.018771065073584253 24 0.01659228073468608 25 0.016424681939386218 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 596663.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.84100793548174 #Duplication Level Percentage of deduplicated Percentage of total 1 95.3169466073847 83.72736663325169 2 3.520459382180574 6.1848140105333 3 0.5353980126944623 1.4108970324520627 4 0.19168946604408624 0.6735278363170734 5 0.10646841059927636 0.46761462501645806 6 0.06921097183737265 0.3647736915833433 7 0.04617361201934464 0.28391556338607754 8 0.028974810988513067 0.2036141281576848 9 0.0196884676038857 0.1556509355127359 >10 0.1376870450849476 2.305916250971952 >50 0.014319915740938974 0.8632370200554247 >100 0.011646781869676413 2.2274306628776945 >500 7.637234012668782E-4 0.5294780967243219 >1k 5.727925509501586E-4 0.6017635131602366 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1342 0.2249175832924113 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1137 0.19055983025594012 No Hit CTGTAGGACGTGGAATATGGCAAGA 1109 0.18586706398754405 No Hit GTCCTACAGTGTGCATTTCTCATTT 963 0.16139763987376457 No Hit CTTTAGGACGTGAAATATGGCGAGG 835 0.13994499407538258 No Hit GTATCAACGCAGAGTACTTTTTTTT 700 0.11731915670990156 No Hit CTGTAGGACCTGGAATATGGCGAGA 659 0.11044760610260734 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.675987952998594E-4 3 0.0 0.0 0.0 0.0 1.675987952998594E-4 4 0.0 0.0 0.0 1.675987952998594E-4 1.675987952998594E-4 5 0.0 0.0 0.0 1.675987952998594E-4 1.675987952998594E-4 6 0.0 0.0 0.0 1.675987952998594E-4 1.675987952998594E-4 7 0.0 0.0 0.0 1.675987952998594E-4 1.675987952998594E-4 8 0.0 0.0 0.0 1.675987952998594E-4 1.675987952998594E-4 9 0.0 0.0 0.0 1.675987952998594E-4 1.675987952998594E-4 10 0.0 0.0 0.0 1.675987952998594E-4 1.675987952998594E-4 11 0.0 0.0 0.0 1.675987952998594E-4 1.675987952998594E-4 12 0.0 0.0 0.0 1.675987952998594E-4 1.675987952998594E-4 13 0.0 0.0 0.0 1.675987952998594E-4 1.675987952998594E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACT 90 2.224624E-9 14.802104 4 TTAGTAC 40 0.0051675113 14.293888 3 ATAGACC 70 7.0179885E-6 13.613226 3 GTCCTAA 400 0.0 13.114877 1 TCCAACG 60 4.136129E-4 12.648171 18 CTAAAAC 55 0.0029948305 12.128147 3 AACACCT 55 0.0030366436 12.105723 7 TATACTC 80 2.8237919E-5 11.88955 5 CTAGGAC 180 0.0 11.646871 3 ACTGTTC 180 0.0 11.617526 8 TAGGACC 370 0.0 11.573074 4 TCCTACA 840 0.0 11.457799 2 TAAAGTG 400 0.0 11.413968 5 TCCTAAA 435 0.0 11.391297 2 CTAAAGT 410 0.0 11.372348 4 GTCCTAC 775 0.0 11.3226385 1 TATACTG 135 4.609319E-9 11.273054 5 GGACCAT 60 0.005815196 11.096912 6 CCTACAC 95 1.3107197E-5 11.033878 3 GGACCTG 515 0.0 10.89683 6 >>END_MODULE