FastQCFastQC Report
Thu 26 May 2016
SRR1926780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926780_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596663
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13440.22525278088301098No Hit
CTGTAGGACGTGGAATATGGCAAGA12790.21435885918852016No Hit
GTATCAACGCAGAGTACTTTTTTTT12270.20564372183292748No Hit
GTCCTAAAGTGTGTATTTCTCATTT11690.19592299170553565No Hit
CTTTAGGACGTGAAATATGGCGAGG11220.18804584832644225No Hit
GTCCTACAGTGTGCATTTCTCATTT9990.16743119650455954No Hit
GGTATCAACGCAGAGTACTTTTTTT8370.14028019166598232No Hit
CTGTAGGACCTGGAATATGGCGAGA8260.13843660491768386No Hit
TATCAACGCAGAGTACTTTTTTTTT7030.11782195309580114No Hit
CTGAAGGACCTGGAATATGGCGAGA6040.10122967236111506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA508.776241E-515.18437719
GGCGAGG2600.014.60036519
TAGGACC3000.014.5945064
TCCAACG707.3219744E-613.5574818
GTATAAG852.5850386E-713.4554921
TGTAGAA2600.012.4614832
ACTGTTC2150.012.3583418
GATATAC2350.012.16721
CTCCTAG657.7948224E-411.730431
GTCCTAA4250.011.6614271
TAGGACT752.0333347E-411.4217874
ATAGGAC1001.879147E-611.4217863
TGTAGGA7600.011.4101672
CTGTAGG7550.011.3614261
AGGACCT5450.011.3519295
GTCCTAC8400.011.3463981
GTAGGAC7600.011.2715013
TACACTG4150.011.2383045
TGGCGAG7300.011.18027818
ATTTCTA5100.011.16498415
Produced by FastQC (version 0.11.5)