##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926780_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 596663 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.62839995106115 31.0 30.0 33.0 25.0 34.0 2 30.0280308985139 31.0 30.0 33.0 26.0 34.0 3 30.136138825434124 31.0 30.0 34.0 26.0 34.0 4 33.7100054804806 35.0 35.0 37.0 28.0 37.0 5 33.64295255445704 37.0 35.0 37.0 28.0 37.0 6 33.5569140369019 37.0 35.0 37.0 28.0 37.0 7 33.5476072757989 37.0 35.0 37.0 28.0 37.0 8 33.56972864079053 37.0 35.0 37.0 28.0 37.0 9 35.104187791098155 39.0 35.0 39.0 29.0 39.0 10 34.97564454306703 39.0 35.0 39.0 28.0 39.0 11 35.00877714890986 39.0 35.0 39.0 28.0 39.0 12 34.91646038048278 39.0 35.0 39.0 27.0 39.0 13 34.93018672181784 39.0 35.0 39.0 27.0 39.0 14 35.983352411662864 40.0 36.0 41.0 27.0 41.0 15 35.974188109535866 40.0 36.0 41.0 27.0 41.0 16 35.951818698327195 40.0 36.0 41.0 27.0 41.0 17 35.929935323624896 40.0 36.0 41.0 27.0 41.0 18 35.89729210626434 40.0 36.0 41.0 27.0 41.0 19 35.96703666894042 40.0 36.0 41.0 27.0 41.0 20 35.93566720242415 40.0 36.0 41.0 27.0 41.0 21 35.8811439623372 40.0 36.0 41.0 27.0 41.0 22 35.79074787610427 40.0 36.0 41.0 27.0 41.0 23 35.71984352976471 40.0 36.0 41.0 27.0 41.0 24 35.64761682893023 40.0 36.0 41.0 27.0 41.0 25 35.53144572396813 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15882767759768512 1101 2 0.09563035804809417 1101 3 0.43552536536779485 1101 4 0.3375881610852218 1101 5 0.33262972028154536 1101 6 0.46574114774396946 1101 7 0.680090668083281 1101 8 0.4729015669502985 1101 9 0.5553599984839295 1101 10 0.5212311859444583 1101 11 0.7465392185302591 1101 12 0.8860246246345653 1101 13 0.832906563388562 1101 14 0.9729922076397344 1101 15 0.6550001071624934 1101 16 0.8399858114697949 1101 17 0.7560586350370926 1101 18 0.8035773421072037 1101 19 0.6845698304331336 1101 20 0.9407681931256917 1101 21 0.9360946135152162 1101 22 0.8123829548325574 1101 23 0.9006300305046651 1101 24 0.9868791536114188 1101 25 0.9967538555355304 1102 1 -0.16504528288519893 1102 2 -0.344983147563525 1102 3 -0.12199844864710485 1102 4 -0.3067318426872916 1102 5 -0.5794942069088762 1102 6 -0.3684237112581954 1102 7 -0.14584774557322788 1102 8 -0.217080042028293 1102 9 -0.20540346468687432 1102 10 -0.13288085781673686 1102 11 -0.19987453229497731 1102 12 -0.21228953173627474 1102 13 -0.35645729589653996 1102 14 -0.4304054156744215 1102 15 -0.25749871392587664 1102 16 -0.17588941252341783 1102 17 -0.2607243509102801 1102 18 -0.25763646530579365 1102 19 -0.38845667150027907 1102 20 -0.4569965904261366 1102 21 -0.5386059429310777 1102 22 -0.589213291512813 1102 23 -0.5183386576024702 1102 24 -0.5210478481805225 1102 25 -0.3222997142088815 1103 1 -0.5717996242629368 1103 2 -0.5530483141669542 1103 3 -0.3788824884610662 1103 4 -0.5487190565930646 1103 5 -0.46398146257587314 1103 6 -0.4819486212329238 1103 7 -0.6116214379099247 1103 8 -0.43150246040896434 1103 9 -0.45692655625850165 1103 10 -0.37095564143393034 1103 11 -0.21741663357668983 1103 12 -0.4065484370194312 1103 13 -0.6205982436138768 1103 14 -0.6335761665889947 1103 15 -0.27978369730490726 1103 16 -0.3283274328981207 1103 17 -0.2658263795513207 1103 18 -0.20531680817739328 1103 19 -0.17756725284041153 1103 20 -0.23242738730191803 1103 21 -0.04607642967606296 1103 22 -0.18872313403719687 1103 23 -0.18727952132007886 1103 24 -0.3011320388121703 1103 25 -0.24590769322804107 1104 1 -0.17922742586662466 1104 2 -0.21329198559292095 1104 3 -0.04872829657128719 1104 4 -0.12388010210938205 1104 5 -0.20409429738876383 1104 6 -0.46875083086565184 1104 7 -0.453453856445897 1104 8 -0.4332191026614254 1104 9 -0.3790564163033281 1104 10 -0.32816430347660486 1104 11 -0.20207156965852846 1104 12 -0.22022392221500553 1104 13 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1107 11 0.3600020009541396 1107 12 0.5318489096227808 1107 13 0.3486010142502778 1107 14 0.5688557662961102 1107 15 0.17795325598659417 1107 16 0.12884017546738846 1107 17 0.11368234709179603 1107 18 0.1403351852897572 1107 19 0.44443214157299593 1107 20 0.16153241250378159 1107 21 0.10020079103219359 1107 22 0.1669490963101694 1107 23 0.14448139122175974 1107 24 0.2900233572800417 1107 25 0.2844715933722455 1108 1 0.8156670713970655 1108 2 0.7188191488811562 1108 3 0.8426581565704936 1108 4 0.7438985154723241 1108 5 0.7951464693459087 1108 6 0.8471970457248403 1108 7 0.6445861169582088 1108 8 0.7505721763852193 1108 9 0.6723088965110051 1108 10 0.5536874073555396 1108 11 0.7808309063739074 1108 12 0.7390752645643346 1108 13 0.5196955944840695 1108 14 0.5656676685277802 1108 15 0.8038831178356958 1108 16 0.717575563352618 1108 17 0.7587407955508851 1108 18 0.7503245582971942 1108 19 0.625091015111785 1108 20 0.7534558981573412 1108 21 0.6878734796613131 1108 22 0.724866205768194 1108 23 0.4983602435065606 1108 24 0.8096832935180842 1108 25 0.7924418377930706 1109 1 0.7191682960585091 1109 2 0.47654758659959384 1109 3 0.7662449654607961 1109 4 0.7691680262328617 1109 5 0.6878843122369034 1109 6 0.830714409776057 1109 7 0.8385247531797688 1109 8 0.7496685978733382 1109 9 0.8198740649800911 1109 10 0.63119398529345 1109 11 0.64819581002061 1109 12 0.6664070938277717 1109 13 0.7666487163762454 1109 14 0.5104311753216635 1109 15 0.9876959918404751 1109 16 0.910277604076164 1109 17 0.9838079956508992 1109 18 1.0390923386196391 1109 19 1.0610636690753097 1109 20 1.0369783768039085 1109 21 1.0253413003322365 1109 22 0.8545882559253286 1109 23 0.9737118719368922 1109 24 0.9988414879522693 1109 25 1.041935208709205 1110 1 0.6103877136418383 1110 2 0.12824468559934488 1110 3 0.3802595397313908 1110 4 0.623164867550642 1110 5 0.4139656067424582 1110 6 0.401233375723244 1110 7 0.601756583830074 1110 8 0.3975974444001693 1110 9 0.38122731094168927 1110 10 0.30200388249773624 1110 11 0.34358247151516963 1110 12 0.39273221643451706 1110 13 0.2081881583423808 1110 14 0.3320764608499118 1110 15 0.17335446625238404 1110 16 0.4038909702893463 1110 17 0.2906047039715389 1110 18 0.24567015580397822 1110 19 0.13437834586454045 1110 20 0.1147262917965648 1110 21 0.2753038948848925 1110 22 0.31951857096231606 1110 23 0.03741196337242769 1110 24 0.29937070576280433 1110 25 0.26901675258441315 1111 1 0.4322310692628477 1111 2 0.24043912586335736 1111 3 0.3931881833364059 1111 4 0.298805011166408 1111 5 0.4503457585279804 1111 6 0.4053677037605681 1111 7 0.3073367442153412 1111 8 0.09988605188625144 1111 9 -0.030040506657691424 1111 10 0.18408523609780758 1111 11 0.25166730801249315 1111 12 0.30178774929629526 1111 13 0.251935067808553 1111 14 0.08967541168086512 1111 15 -0.05259956810360933 1111 16 0.2787689613886073 1111 17 -0.06699747176428161 1111 18 0.06637726994411253 1111 19 0.16769268809144222 1111 20 0.041119988182522604 1111 21 0.10225012497110697 1111 22 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0.14287167353095498 2214 13 0.20981401109474263 2214 14 0.12415466267370334 2214 15 0.07067382035697278 2214 16 0.07462297450216937 2214 17 -0.11981514921480851 2214 18 0.026810898975348607 2214 19 0.01628808774982815 2214 20 0.031366407807929875 2214 21 0.20639039003961557 2214 22 0.20817835386439754 2214 23 0.2550433626537085 2214 24 0.05469680249893116 2214 25 0.05350764156386134 2215 1 0.29237945134886445 2215 2 0.1744588729530001 2215 3 0.18281752848995936 2215 4 0.3226124405690598 2215 5 0.2721445802248894 2215 6 0.3154309098949568 2215 7 -0.025294656317598196 2215 8 -0.07501107186990197 2215 9 -0.0035458510731984916 2215 10 0.004534544606393354 2215 11 -0.1329300049758757 2215 12 -0.14879732581730565 2215 13 -0.08999595898003321 2215 14 -0.12050965828355942 2215 15 0.018896739508164728 2215 16 -0.2597747658423799 2215 17 0.10493458485949247 2215 18 -0.1031647108580458 2215 19 -0.11198158362198996 2215 20 -0.22248885466892432 2215 21 0.02364688184847097 2215 22 -0.18543076757200794 2215 23 0.15782282759112576 2215 24 0.02022522618010214 2215 25 -0.039889056204053475 2216 1 -1.387632746036651 2216 2 -1.2374521391648372 2216 3 -3.2419272544761277 2216 4 -2.786241270352903 2216 5 -1.694962252962604 2216 6 -1.3893870573842335 2216 7 -1.267261617312485 2216 8 -1.1406714542314234 2216 9 -1.2553144719731506 2216 10 -1.4744209879685855 2216 11 -1.4245588862634975 2216 12 -1.612442487962987 2216 13 -1.411268464482113 2216 14 -1.8112608581350713 2216 15 -1.7620846197048436 2216 16 -1.655415526322848 2216 17 -1.687308143112297 2216 18 -1.448039157335387 2216 19 -1.757570637794288 2216 20 -1.5890534345220217 2216 21 -1.6200926774546858 2216 22 -1.5016917385899617 2216 23 -1.6708582638097127 2216 24 -1.8751609801678413 2216 25 -1.6433520530486447 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15824.0 3 23.0 4 288.0 5 1780.0 6 2402.0 7 1702.0 8 1312.0 9 1170.0 10 1060.0 11 969.0 12 810.0 13 835.0 14 814.0 15 805.0 16 875.0 17 871.0 18 1010.0 19 1185.0 20 1473.0 21 1938.0 22 2558.0 23 2856.0 24 3518.0 25 4186.0 26 4918.0 27 5380.0 28 6352.0 29 7553.0 30 9632.0 31 12531.0 32 16191.0 33 21068.0 34 28406.0 35 40161.0 36 59224.0 37 102455.0 38 232528.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.50982968149382 19.872449878439802 10.284714287392136 28.33300615267424 2 13.431513526483316 21.857313764411163 41.22460690729036 23.486565801815164 3 18.10920538282565 25.991008508726644 29.693545034564607 26.206241073883103 4 11.356661968313771 18.334135014237518 39.59521538959178 30.71398762785693 5 11.719362819043022 39.534684929302685 36.696735654377605 12.049216597276695 6 29.85301784302844 39.84225484755226 18.578611909927183 11.726115399492121 7 26.672466887611503 32.50638275758597 23.0813589951201 17.73979135968243 8 23.579854976751356 39.808479468449235 20.75682960271139 15.85483595208802 9 25.910089005849912 15.685981997686854 20.55733418260447 37.846594813858765 10 13.30332046668555 30.97264147306636 35.75145860185407 19.972579458394023 11 33.40020883929933 23.24787012408004 25.479400165261275 17.87252087135935 12 22.409840076961494 25.87394538281305 33.2538355048587 18.46237903536676 13 28.536448015446037 23.197208451072473 26.385802251876285 21.880541281605208 14 19.431571925860997 23.8377442542943 28.651684451692162 28.07899936815254 15 21.52374599428809 33.31947815127177 23.685489601909335 21.471286252530806 16 20.173633224952233 29.82334998156404 29.666476720410284 20.336540073073444 17 20.019508499772904 30.30906893841247 29.231408684634374 20.440013877180252 18 19.841684851288342 29.219586429747025 31.420177052851045 19.518551666113588 19 22.228125424234452 28.15324563447038 28.39576109126928 21.222867850025896 20 22.043318020236082 28.99186634867269 29.45628728063192 19.508528350459308 21 21.402123718770692 28.08337314258417 28.146229854423854 22.36827328422129 22 21.40370695015444 30.055994757509687 28.367269296068297 20.173028996267576 23 20.599232732715116 29.608841171649658 28.89386471090046 20.898061384734767 24 21.806446855260003 28.37900121173929 29.776775164540116 20.03777676846059 25 21.185075682533935 28.99836919265818 29.23871633841012 20.57783878639776 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 233.0 1 233.0 2 264.5 3 296.0 4 296.0 5 296.0 6 477.5 7 659.0 8 659.0 9 659.0 10 1055.0 11 1451.0 12 1451.0 13 1451.0 14 2353.5 15 3256.0 16 3256.0 17 3256.0 18 5661.0 19 8066.0 20 8066.0 21 8066.0 22 12904.0 23 17742.0 24 17742.0 25 17742.0 26 25847.0 27 33952.0 28 33952.0 29 33952.0 30 44713.5 31 55475.0 32 55475.0 33 55475.0 34 62191.5 35 68908.0 36 68908.0 37 68908.0 38 73307.0 39 77706.0 40 77706.0 41 77706.0 42 79082.0 43 80458.0 44 80458.0 45 80458.0 46 79804.5 47 79151.0 48 79151.0 49 79151.0 50 72857.5 51 66564.0 52 66564.0 53 66564.0 54 55716.0 55 44868.0 56 44868.0 57 44868.0 58 36802.5 59 28737.0 60 28737.0 61 28737.0 62 22329.5 63 15922.0 64 15922.0 65 15922.0 66 11976.5 67 8031.0 68 8031.0 69 8031.0 70 5687.0 71 3343.0 72 3343.0 73 3343.0 74 2284.5 75 1226.0 76 1226.0 77 1226.0 78 834.0 79 442.0 80 442.0 81 442.0 82 289.0 83 136.0 84 136.0 85 136.0 86 82.5 87 29.0 88 29.0 89 29.0 90 20.5 91 12.0 92 12.0 93 12.0 94 6.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11095040248850692 2 0.11463757598510382 3 0.01659228073468608 4 0.0 5 0.005865957835495078 6 0.3407283508446141 7 0.022458238570181158 8 0.011564316875690298 9 0.012234712056889734 10 0.0050279638589957815 11 0.0053631614495955006 12 1.675987952998594E-4 13 8.379939764992969E-4 14 0.0 15 0.0025139819294978908 16 5.027963858995782E-4 17 0.0 18 8.379939764992969E-4 19 0.0 20 0.0010055927717991563 21 0.011396718080390437 22 0.0 23 0.0 24 0.0 25 0.004357568677796344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 596663.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.84720945043246 #Duplication Level Percentage of deduplicated Percentage of total 1 93.72257527690114 79.52098974753154 2 4.821113765420956 8.1811609887807 3 0.6914488003412698 1.7600250356041791 4 0.24043297761568513 0.8160026884219669 5 0.13023969543584585 0.5525237358702872 6 0.07845402533864676 0.39939630720826175 7 0.05047666722082183 0.29979630492313936 8 0.039585687848543515 0.2686988118499849 9 0.034120787530512155 0.26055482455935625 >10 0.15872123792511106 2.5993411067542342 >50 0.017404468107385086 1.0543436843398533 >100 0.01305328565052651 2.4157684647471833 >500 0.0013844393871171735 0.8408874532227822 >1k 9.888852765122668E-4 1.0305108461866106 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1344 0.22525278088301098 No Hit CTGTAGGACGTGGAATATGGCAAGA 1279 0.21435885918852016 No Hit GTATCAACGCAGAGTACTTTTTTTT 1227 0.20564372183292748 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1169 0.19592299170553565 No Hit CTTTAGGACGTGAAATATGGCGAGG 1122 0.18804584832644225 No Hit GTCCTACAGTGTGCATTTCTCATTT 999 0.16743119650455954 No Hit GGTATCAACGCAGAGTACTTTTTTT 837 0.14028019166598232 No Hit CTGTAGGACCTGGAATATGGCGAGA 826 0.13843660491768386 No Hit TATCAACGCAGAGTACTTTTTTTTT 703 0.11782195309580114 No Hit CTGAAGGACCTGGAATATGGCGAGA 604 0.10122967236111506 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.675987952998594E-4 0.0 11 0.0 0.0 0.0 1.675987952998594E-4 0.0 12 0.0 0.0 0.0 1.675987952998594E-4 0.0 13 0.0 0.0 0.0 1.675987952998594E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 50 8.776241E-5 15.184377 19 GGCGAGG 260 0.0 14.600365 19 TAGGACC 300 0.0 14.594506 4 TCCAACG 70 7.3219744E-6 13.55748 18 GTATAAG 85 2.5850386E-7 13.455492 1 TGTAGAA 260 0.0 12.461483 2 ACTGTTC 215 0.0 12.358341 8 GATATAC 235 0.0 12.1672 1 CTCCTAG 65 7.7948224E-4 11.73043 1 GTCCTAA 425 0.0 11.661427 1 TAGGACT 75 2.0333347E-4 11.421787 4 ATAGGAC 100 1.879147E-6 11.421786 3 TGTAGGA 760 0.0 11.410167 2 CTGTAGG 755 0.0 11.361426 1 AGGACCT 545 0.0 11.351929 5 GTCCTAC 840 0.0 11.346398 1 GTAGGAC 760 0.0 11.271501 3 TACACTG 415 0.0 11.238304 5 TGGCGAG 730 0.0 11.180278 18 ATTTCTA 510 0.0 11.164984 15 >>END_MODULE