FastQCFastQC Report
Thu 26 May 2016
SRR1926779_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926779_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911691
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT24180.26522144015900123No Hit
CTGTAGGACGTGGAATATGGCAAGA18660.20467461014751712No Hit
GTCCTACAGTGTGCATTTCTCATTT17750.19469315809852242No Hit
GTCCTAAAGTGTGTATTTCTCATTT16560.18164049003445246No Hit
CTTTAGGACGTGAAATATGGCGAGG13260.1454440155710652No Hit
CTGTAGGACCTGGAATATGGCGAGA12490.13699817152960816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGACG250.006091711618.96664419
ACATTCG456.8491645E-414.75183518
CGCCTTG801.233002E-714.2995092
GGGCCGC400.005217512614.2743196
GTCCTAG1450.013.1584081
TCGCCTT801.9051076E-613.1172881
TCCAACG1102.8121576E-912.93180518
TGTAGGA12100.012.7631972
TCTAGTA751.425234E-512.7106742
GTTATAC603.9711953E-412.7106743
CTGTAGG11950.012.6931841
GTAGGAC12200.012.5804423
GTATAAG1154.989488E-912.4432771
TAGGACC4800.012.2992224
GTCTAGT550.002972096412.1416231
GGACGTG8800.012.0034056
GGCGAGG3900.011.91494519
GGACCTG7200.011.7630966
AGGACGT9100.011.7122625
GTCCTAC13500.011.6598111
Produced by FastQC (version 0.11.5)