FastQCFastQC Report
Thu 26 May 2016
SRR1926779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926779_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911691
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT25200.27640944135677553No Hit
CTGTAGGACGTGGAATATGGCAAGA24180.26522144015900123No Hit
GTCCTACAGTGTGCATTTCTCATTT19690.21597229763154402No Hit
GTCCTAAAGTGTGTATTTCTCATTT18810.20631990444130743No Hit
CTTTAGGACGTGAAATATGGCGAGG16720.1833954706144955No Hit
CTGTAGGACCTGGAATATGGCGAGA15500.17001374369166747No Hit
GTATCAACGCAGAGTACTTTTTTTT13110.14379872127727486No Hit
CTGAAGGACCTGGAATATGGCGAGA9810.10760224681388761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5150.014.2361874
CGGTTTC759.802716E-713.91523313
TCCAACG1205.2750693E-1113.441586518
TGCCGGT751.4960231E-512.65090510
CCGGTTT751.4968671E-512.65021112
CCAACGA853.9934584E-612.2788219
CCCCTAG550.002986538712.1337711
CTAGGAC1650.012.1197423
TGTAGGA13900.012.0700872
GTAGGAC13350.012.0548953
GGACGTG11100.012.00923256
AGGACGT11200.011.9870215
GGCGAGG5100.011.906733519
GACTGTA802.8935216E-511.8628267
TAGGACG11800.011.7002794
GACGTGG5850.011.6803227
CTGTAGG13750.011.648421
AGGACCT8900.011.5543095
CTAATAC1001.8720675E-611.4271853
GGACCTG8100.011.4024186
Produced by FastQC (version 0.11.5)