##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926779_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 911691 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.768205455576506 31.0 30.0 33.0 25.0 34.0 2 30.059636433835585 31.0 30.0 34.0 26.0 34.0 3 30.153397368187246 31.0 30.0 34.0 26.0 34.0 4 33.76041553552684 35.0 35.0 37.0 28.0 37.0 5 33.705833445761776 37.0 35.0 37.0 28.0 37.0 6 33.61409293280289 37.0 35.0 37.0 28.0 37.0 7 33.58175302816415 37.0 35.0 37.0 28.0 37.0 8 33.61272075736187 37.0 35.0 37.0 28.0 37.0 9 35.151016078912704 39.0 35.0 39.0 29.0 39.0 10 35.03934118029025 39.0 35.0 39.0 28.0 39.0 11 35.06889066580673 39.0 35.0 39.0 29.0 39.0 12 34.96163173706881 39.0 35.0 39.0 28.0 39.0 13 34.9683116319016 39.0 35.0 39.0 28.0 39.0 14 36.05297957312291 40.0 36.0 41.0 27.0 41.0 15 36.04685578776142 40.0 36.0 41.0 27.0 41.0 16 36.02584647649258 40.0 36.0 41.0 27.0 41.0 17 35.99829766883736 40.0 36.0 41.0 27.0 41.0 18 35.97531839186742 40.0 36.0 41.0 27.0 41.0 19 36.03694124434704 40.0 36.0 41.0 27.0 41.0 20 35.99622898547863 40.0 36.0 41.0 27.0 41.0 21 35.94413238696006 40.0 36.0 41.0 27.0 41.0 22 35.85389018867138 40.0 36.0 41.0 27.0 41.0 23 35.77983658937074 40.0 36.0 41.0 27.0 41.0 24 35.70719794316276 40.0 36.0 41.0 27.0 41.0 25 35.59076485344267 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13257932583712773 1101 2 0.13171424545090815 1101 3 0.3020574066502668 1101 4 0.22241999946938762 1101 5 0.08599445009056694 1101 6 0.09849478973746528 1101 7 0.06255226353300003 1101 8 0.13013476713205563 1101 9 0.09201964259161599 1101 10 0.09691602811403044 1101 11 0.1671245583549208 1101 12 0.2685030687954182 1101 13 0.2863479201198942 1101 14 0.2160640479818312 1101 15 0.10609689842240044 1101 16 0.20949954283034344 1101 17 0.316025123947945 1101 18 0.22060088143555845 1101 19 0.22109440751760445 1101 20 0.2869375954873803 1101 21 0.32910969034190884 1101 22 0.25392273344768057 1101 23 0.2826654851753858 1101 24 0.3626606532578407 1101 25 0.26958305480611955 1102 1 -0.2430775105075007 1102 2 -0.26471816687554295 1102 3 -0.12997972472460972 1102 4 -0.09111370744179936 1102 5 -0.13492267546232029 1102 6 -0.13381241740417948 1102 7 -0.17598771010631253 1102 8 0.02831296862152044 1102 9 0.0527163410366569 1102 10 -0.03554238911259944 1102 11 0.052036702869713736 1102 12 0.2726237457637808 1102 13 0.113245525825441 1102 14 0.05705249294378234 1102 15 2.5145615311572556E-4 1102 16 -0.0814108712588748 1102 17 -0.02162942165362125 1102 18 0.03470465845324355 1102 19 0.047930558857068206 1102 20 0.10876198155017391 1102 21 0.030941192173408183 1102 22 0.07722040707648148 1102 23 0.03444806609703477 1102 24 0.134255798362922 1102 25 0.048363509083266365 1103 1 -0.7822140810914817 1103 2 -0.7161138150572661 1103 3 -0.4335112034140174 1103 4 -0.5352501401892198 1103 5 -0.6829528849950961 1103 6 -0.8478036764457286 1103 7 -0.7189025928498083 1103 8 -0.7032413188376907 1103 9 -0.6501149279291241 1103 10 -0.47786149983353 1103 11 -0.5699910637751131 1103 12 -0.7532068255369566 1103 13 -0.5535102044644589 1103 14 -0.5543220980527295 1103 15 -0.576490638920184 1103 16 -0.6610591971808475 1103 17 -0.5832222185270624 1103 18 -0.46545120288742226 1103 19 -0.686256406893726 1103 20 -0.6353444452384664 1103 21 -0.5447072228121854 1103 22 -0.4790041859345706 1103 23 -0.5700564326762176 1103 24 -0.5892785218801819 1103 25 -0.5737575623097086 1104 1 -0.12829252876013797 1104 2 -0.10806750608282556 1104 3 -0.03211152463473965 1104 4 0.08567772228010284 1104 5 -0.10254433628527693 1104 6 -0.3021876328627684 1104 7 -0.24964815320959133 1104 8 -0.31179408027624333 1104 9 -0.30510964004596275 1104 10 -0.3671260581342608 1104 11 -0.19758384067215218 1104 12 0.024186798668047516 1104 13 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2214 7 0.5051103659325875 2214 8 0.37727234993674585 2214 9 0.32028525632618 2214 10 0.20618918560383293 2214 11 0.20964491778412508 2214 12 0.28321024625028457 2214 13 0.3718473612335984 2214 14 0.24032745520428733 2214 15 0.1320490616268728 2214 16 0.18503242034472578 2214 17 0.21045561639693489 2214 18 0.40425478807492965 2214 19 0.3033402110315748 2214 20 0.2914464287940959 2214 21 0.4133718394653485 2214 22 0.3751942844418181 2214 23 0.4678034647651472 2214 24 0.39050383605479055 2214 25 0.5006450355378504 2215 1 0.33723157879052934 2215 2 0.13766087299439533 2215 3 0.23023904176907806 2215 4 0.08204545255033935 2215 5 0.23934293406732365 2215 6 0.10918366456246531 2215 7 0.25110585653686 2215 8 0.14543356083399317 2215 9 0.081477479693433 2215 10 0.25210704128933514 2215 11 0.06611195838944184 2215 12 0.09018718746906984 2215 13 0.15110829063942788 2215 14 0.09256520825262271 2215 15 0.10125013087929347 2215 16 0.019120201403879378 2215 17 0.14076574109694207 2215 18 0.14001336249724972 2215 19 0.10500979405079391 2215 20 0.04118361125171788 2215 21 0.17361134924146882 2215 22 0.24318639299929856 2215 23 0.15094406523406434 2215 24 0.1589693620011019 2215 25 0.1820928835463178 2216 1 -0.362497590196881 2216 2 -0.14216883069280684 2216 3 -1.8756635426132355 2216 4 -1.5387911298268477 2216 5 -0.521445051896805 2216 6 -0.18507527494962517 2216 7 -0.10938484191473918 2216 8 -0.22633248015965535 2216 9 -0.2828215623027859 2216 10 -0.40900956225305407 2216 11 -0.27500631608978665 2216 12 -0.1703710446797615 2216 13 -0.1868108360175853 2216 14 -0.5260190905108857 2216 15 -0.4875079453154214 2216 16 -0.5373679070956143 2216 17 -0.442144056162725 2216 18 -0.42279645704157076 2216 19 -0.5054321687430132 2216 20 -0.32082485482826684 2216 21 -0.18115987165637648 2216 22 -0.34245956333354854 2216 23 -0.3766298737210434 2216 24 -0.60266453573756 2216 25 -0.40211537088166693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23718.0 3 19.0 4 365.0 5 2897.0 6 3851.0 7 2699.0 8 2000.0 9 1749.0 10 1600.0 11 1466.0 12 1308.0 13 1256.0 14 1259.0 15 1255.0 16 1186.0 17 1317.0 18 1460.0 19 1725.0 20 2240.0 21 2882.0 22 3621.0 23 4552.0 24 5290.0 25 6266.0 26 7421.0 27 8049.0 28 9416.0 29 11339.0 30 14604.0 31 18662.0 32 24307.0 33 31893.0 34 42464.0 35 60012.0 36 88489.0 37 153388.0 38 365666.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.06136300496662 19.001646024559697 9.719560899065423 29.217430071408256 2 14.001579034636642 20.798785087675952 40.50322669908189 24.696409178605506 3 18.931289000447602 24.0237271921433 28.983969773830314 28.06101403357878 4 11.645063952589199 17.110621910274425 38.391187365017316 32.85312677211906 5 12.529644182061897 38.73444297457566 36.0498482963782 12.686064546984246 6 31.89924515303287 38.47419444655378 17.478135410235502 12.148424990177856 7 28.42432201446647 31.340106880295604 21.53821173532919 18.69735936990873 8 24.544421383344233 39.445122138509944 19.561797654203435 16.448658823942395 9 26.07400256694347 15.501047620092365 19.532684649897433 38.89226516306673 10 13.745626062633686 30.34454011956343 34.912960017550596 20.99687380025229 11 34.80364088692321 22.545044896813017 24.488452754687675 18.162861461576092 12 23.262453821921532 24.935284878160072 32.297672010600124 19.504589289318275 13 29.472647380786803 22.35232305859693 25.413135662936583 22.761893897679684 14 19.73124666142366 23.167279264575388 27.573487069632147 29.527987004368804 15 22.052592148143436 32.90634525923675 22.7157801673217 22.32528242529811 16 21.107729095027338 28.91865063042608 28.908120677646338 21.065499596900246 17 20.701531549614945 29.71664741672343 28.332735543073255 21.249085490588367 18 20.625764381337852 28.375151505179968 30.64731787843389 20.35176623504829 19 22.975792210071404 27.3412015048975 27.378604569535696 22.3044017154954 20 22.810804644601955 28.3524299042868 28.482628811361856 20.354136639749388 21 21.928821859381376 27.35307797957813 27.201471743318134 23.51662841772235 22 22.12021397600722 29.57186151886988 27.295103275122823 21.012821230000075 23 21.190207197621998 29.006131470126906 27.972227401858085 21.831433930393008 24 22.577715475967185 27.48431211890871 29.01783608700755 20.920136318116555 25 21.835473237938015 28.391095217985068 28.365646715552657 21.407784828524264 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 248.0 1 248.0 2 238.5 3 229.0 4 229.0 5 229.0 6 425.0 7 621.0 8 621.0 9 621.0 10 1037.0 11 1453.0 12 1453.0 13 1453.0 14 2421.0 15 3389.0 16 3389.0 17 3389.0 18 6259.0 19 9129.0 20 9129.0 21 9129.0 22 14939.0 23 20749.0 24 20749.0 25 20749.0 26 31446.0 27 42143.0 28 42143.0 29 42143.0 30 57135.5 31 72128.0 32 72128.0 33 72128.0 34 82861.5 35 93595.0 36 93595.0 37 93595.0 38 101869.0 39 110143.0 40 110143.0 41 110143.0 42 115429.0 43 120715.0 44 120715.0 45 120715.0 46 123034.0 47 125353.0 48 125353.0 49 125353.0 50 118433.0 51 111513.0 52 111513.0 53 111513.0 54 96628.0 55 81743.0 56 81743.0 57 81743.0 58 69057.0 59 56371.0 60 56371.0 61 56371.0 62 44959.0 63 33547.0 64 33547.0 65 33547.0 66 25369.0 67 17191.0 68 17191.0 69 17191.0 70 12261.0 71 7331.0 72 7331.0 73 7331.0 74 5028.0 75 2725.0 76 2725.0 77 2725.0 78 1836.5 79 948.0 80 948.0 81 948.0 82 626.0 83 304.0 84 304.0 85 304.0 86 199.0 87 94.0 88 94.0 89 94.0 90 58.0 91 22.0 92 22.0 93 22.0 94 13.0 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11100252168772096 2 0.1105637765427102 3 0.017878864659188257 4 0.0 5 0.004935882881370991 6 0.33234944734564675 7 0.022376002395548492 8 0.011297687484026934 9 0.011078314911521558 10 0.004497137736360236 11 0.004935882881370991 12 3.290588587580661E-4 13 5.484314312634434E-4 14 0.0 15 0.0037293337325914155 16 6.581177175161322E-4 17 0.0 18 6.581177175161322E-4 19 1.0968628625268869E-4 20 9.871765762741983E-4 21 0.013052668064069954 22 0.0 23 1.0968628625268869E-4 24 0.0 25 0.0050455691676236794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 911691.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.06703054389983 #Duplication Level Percentage of deduplicated Percentage of total 1 92.35488189632544 76.71645795360327 2 5.916969714670441 9.830102080317197 3 0.8679234116758335 2.1628746164232657 4 0.2946098049948693 0.9788944668016472 5 0.1466663674447209 0.6091569812146733 6 0.08573503927274051 0.42730530755707125 7 0.05847166249117976 0.33999471618752014 8 0.04328247113971147 0.28762770817423006 9 0.03167088144499172 0.23677254687090218 >10 0.16594209074999383 2.5817367145979313 >50 0.01705916345375511 0.9698927209848609 >100 0.013615056777207585 2.213611448693577 >500 0.0022471446876596098 1.1828984441211556 >1k 9.252948713892509E-4 1.462674294452605 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2520 0.27640944135677553 No Hit CTGTAGGACGTGGAATATGGCAAGA 2418 0.26522144015900123 No Hit GTCCTACAGTGTGCATTTCTCATTT 1969 0.21597229763154402 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1881 0.20631990444130743 No Hit CTTTAGGACGTGAAATATGGCGAGG 1672 0.1833954706144955 No Hit CTGTAGGACCTGGAATATGGCGAGA 1550 0.17001374369166747 No Hit GTATCAACGCAGAGTACTTTTTTTT 1311 0.14379872127727486 No Hit CTGAAGGACCTGGAATATGGCGAGA 981 0.10760224681388761 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.0968628625268869E-4 0.0 11 0.0 0.0 0.0 1.0968628625268869E-4 0.0 12 0.0 0.0 0.0 1.0968628625268869E-4 1.0968628625268869E-4 13 0.0 0.0 0.0 1.0968628625268869E-4 1.0968628625268869E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 515 0.0 14.236187 4 CGGTTTC 75 9.802716E-7 13.915233 13 TCCAACG 120 5.2750693E-11 13.4415865 18 TGCCGGT 75 1.4960231E-5 12.650905 10 CCGGTTT 75 1.4968671E-5 12.650211 12 CCAACGA 85 3.9934584E-6 12.27882 19 CCCCTAG 55 0.0029865387 12.133771 1 CTAGGAC 165 0.0 12.119742 3 TGTAGGA 1390 0.0 12.070087 2 GTAGGAC 1335 0.0 12.054895 3 GGACGTG 1110 0.0 12.0092325 6 AGGACGT 1120 0.0 11.987021 5 GGCGAGG 510 0.0 11.9067335 19 GACTGTA 80 2.8935216E-5 11.862826 7 TAGGACG 1180 0.0 11.700279 4 GACGTGG 585 0.0 11.680322 7 CTGTAGG 1375 0.0 11.64842 1 AGGACCT 890 0.0 11.554309 5 CTAATAC 100 1.8720675E-6 11.427185 3 GGACCTG 810 0.0 11.402418 6 >>END_MODULE