FastQCFastQC Report
Thu 26 May 2016
SRR1926778_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926778_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909694
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT20520.2255703566254147No Hit
CTGTAGGACGTGGAATATGGCAAGA16300.17918113123753704No Hit
GTCCTAAAGTGTGTATTTCTCATTT16030.1762131002293079No Hit
GTCCTACAGTGTGCATTTCTCATTT13250.1456533735519856No Hit
CTTTAGGACGTGAAATATGGCGAGG11770.12938416654391477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG250.005953031619.0424024
CGCCTTG659.613359E-917.596062
TCGCCTT607.318158E-817.4893321
AGAACCG350.002142720916.3157545
GAACCTA400.005212733514.2762846
TCCAACG759.857285E-713.90837218
CCAACGA551.9887622E-413.79342619
GTCTAAA706.949118E-613.6280521
AGTCCCG500.001478037113.32453255
TAGGACC3750.013.2027334
CCTAAAC751.4280049E-512.7082663
GTCTAAT550.002972577512.14135551
GTCTAGA907.1133236E-611.65955451
AGGACCT6700.011.6483125
GGCGAGG4100.011.56461119
CCTATAC1251.740591E-811.437443
TTAGGAC5250.011.4374393
CTATACC1251.7678758E-811.4254424
TGGCGAG9650.011.3992318
GTCCTAC10750.011.3588221
Produced by FastQC (version 0.11.5)