FastQCFastQC Report
Thu 26 May 2016
SRR1926778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926778_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909694
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT22440.24667635490615525No Hit
CTGTAGGACGTGGAATATGGCAAGA20680.22732918981547642No Hit
GTCCTAAAGTGTGTATTTCTCATTT16670.18324843298955473No Hit
CTTTAGGACGTGAAATATGGCGAGG15340.16862813209716673No Hit
GTATCAACGCAGAGTACTTTTTTTT15150.16653951768396844No Hit
GTCCTACAGTGTGCATTTCTCATTT14110.15510710194856733No Hit
CTGTAGGACCTGGAATATGGCGAGA10850.11927087570105992No Hit
GGTATCAACGCAGAGTACTTTTTTT9800.10772853289127993No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG400.005196455414.2830175
TAGAACG400.005196455414.2830174
TAGGACC3600.014.2830174
GCTATAG603.971011E-412.710741
CCTATAC1353.5470293E-1011.9920033
CTAAATT6750.011.94849619
TGTAGGA10650.011.90252
TCCAACG1201.0168151E-811.86063918
CCAACGA1052.7578426E-711.7476819
GGACGTG9250.011.7345886
TATGTCG658.0996077E-411.67816716
AGGACGT9300.011.56975655
GACGTGG5250.011.5688547
GTAGGAC11000.011.5142473
AGGACCT7700.011.3769485
GTCCTAC11400.011.3727681
CCCTATA855.1436655E-511.2128822
AATGCCG855.3829397E-511.16295318
TAAGACT1201.2323471E-711.1090144
GTCGAGG600.005925614411.06992919
Produced by FastQC (version 0.11.5)