FastQCFastQC Report
Thu 26 May 2016
SRR1926777_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926777_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955279
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT22820.23888309069915703No Hit
CTGTAGGACGTGGAATATGGCAAGA17260.18068019918788125No Hit
GTCCTAAAGTGTGTATTTCTCATTT15500.16225626230661408No Hit
GTCCTACAGTGTGCATTTCTCATTT14140.14801958380745311No Hit
CTTTAGGACGTGAAATATGGCGAGG12040.12603647730139572No Hit
CTGTAGGACCTGGAATATGGCGAGA9980.10447209663354894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTA400.005163512714.2968152
TCGAACT551.9883669E-413.79388319
CGCCTTG707.0137467E-613.61601452
CCTACAC1950.013.1963653
TAGGACC4350.013.1319834
TCGCCTT801.9022227E-613.1192241
CTACACT2200.012.5499974
GGCGAGG4050.011.94192719
TAGGACA3750.011.678714
GTAGGAC11400.011.3699693
AGGACCT6850.011.2550815
GTCCTAC11650.011.2201871
TGTAGGA12150.011.2178032
AGGACGT9000.011.2103075
TCCAACG1105.0740164E-711.2075318
TATACTG2050.011.1432425
GACGTGA5000.011.0410967
GGACGTG9000.010.8930356
CTGTAGG12200.010.8707721
GGACATG4050.010.8107796
Produced by FastQC (version 0.11.5)