##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926777_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 955279 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.782934619100807 30.0 27.0 31.0 10.0 31.0 2 27.42680724688808 31.0 28.0 31.0 15.0 34.0 3 27.059146071461846 31.0 27.0 31.0 10.0 34.0 4 30.80932481505403 35.0 32.0 37.0 12.0 37.0 5 30.406544056762474 35.0 30.0 37.0 10.0 37.0 6 30.19339690289434 35.0 30.0 37.0 10.0 37.0 7 30.227951205878075 35.0 30.0 37.0 10.0 37.0 8 29.963638895024385 35.0 30.0 37.0 10.0 37.0 9 32.184033146337356 37.0 32.0 39.0 10.0 39.0 10 32.36959568879877 37.0 32.0 39.0 10.0 39.0 11 32.579122957795576 37.0 33.0 39.0 10.0 39.0 12 32.42901916612843 37.0 32.0 39.0 10.0 39.0 13 32.552638548528755 37.0 33.0 39.0 10.0 39.0 14 33.50428932280517 39.0 33.0 40.0 8.0 41.0 15 33.08144008190278 38.0 32.0 40.0 7.0 41.0 16 33.15640456871762 38.0 32.0 40.0 2.0 41.0 17 33.379780147998645 38.0 33.0 40.0 2.0 41.0 18 33.43369214648286 39.0 33.0 40.0 2.0 41.0 19 32.79500753183102 39.0 32.0 40.0 2.0 41.0 20 33.07001933466558 39.0 32.0 40.0 2.0 41.0 21 33.051779637153125 39.0 33.0 40.0 2.0 41.0 22 33.07852051599585 39.0 33.0 40.0 2.0 41.0 23 33.06324853786171 39.0 33.0 40.0 2.0 41.0 24 31.763321500839023 38.0 31.0 40.0 2.0 41.0 25 32.325714267768895 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.177036436735051 1101 2 -1.1715591336017397 1101 3 -2.2623400274463776 1101 4 -1.3500039698550452 1101 5 -1.7284252046578032 1101 6 -2.93972922913877 1101 7 -1.7641310044673872 1101 8 -1.38295567431474 1101 9 -1.4128675058934625 1101 10 -9.566075825874044 1101 11 -4.764622855263148 1101 12 -2.5608806470680676 1101 13 -2.022712097906851 1101 14 -1.6160773151538557 1101 15 -3.136190223902389 1101 16 -1.488970953998905 1101 17 -1.3512334734304616 1101 18 -1.3472622534188687 1101 19 -1.9953101402851274 1101 20 -1.297572657929983 1101 21 -1.4037018499125473 1101 22 -0.9242851309928497 1101 23 -1.3294326157739995 1101 24 -1.482537770463754 1101 25 -1.3536775317785654 1102 1 -0.8390057740544705 1102 2 -0.7902216579334755 1102 3 -1.1616968122531262 1102 4 -0.721305381032348 1102 5 -0.9438086961611631 1102 6 -1.491819310718892 1102 7 -0.6370621410239146 1102 8 -0.09208634709074204 1102 9 -0.18701318736217587 1102 10 -0.20553034854587082 1102 11 -0.3683832214580036 1102 12 -0.4416522212077645 1102 13 -0.27319213763917816 1102 14 -0.7360722826633435 1102 15 0.06078916571536297 1102 16 -0.2716896250967338 1102 17 -0.4380277008797506 1102 18 -0.45098837635672595 1102 19 -0.08028567030733313 1102 20 -0.160515416901589 1102 21 -0.35599465157407906 1102 22 -0.4013322563094732 1102 23 -0.8309370361980015 1102 24 -4.38783360430228 1102 25 -2.3247209263557522 1103 1 -0.676617054839447 1103 2 -0.500305524341627 1103 3 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25 -2.469541795265254 1106 1 -0.6008594790818691 1106 2 -1.5002330221158076 1106 3 -0.9991159564390095 1106 4 -1.287103898688013 1106 5 -0.7378385293459466 1106 6 -0.7208453345983834 1106 7 -3.552450459742655 1106 8 -1.2533523307298502 1106 9 -1.4231925669111583 1106 10 -0.733548597912705 1106 11 -1.130097693190212 1106 12 -0.723516300096172 1106 13 -0.9509249097250567 1106 14 -1.2695221013168805 1106 15 -0.5881198629755247 1106 16 -0.718864807056022 1106 17 -1.2297087075953073 1106 18 -1.4263754596251061 1106 19 -0.8140922007410829 1106 20 -0.8297681949490112 1106 21 -1.20688265603739 1106 22 -1.6347750152466354 1106 23 -1.5188956113511303 1106 24 -4.282405074615681 1106 25 -2.836942570073436 1107 1 0.2558737166052154 1107 2 0.07623097980255622 1107 3 0.4876122262056697 1107 4 0.1868046789013249 1107 5 0.11095168474211903 1107 6 0.13146533808393457 1107 7 -1.9554490332441468 1107 8 -1.7436406231516592 1107 9 -0.5787786161145014 1107 10 -0.00927867531747495 1107 11 -0.26750839542210514 1107 12 -0.193174176473228 1107 13 -0.464243458831632 1107 14 -0.41935259251007295 1107 15 -1.6173508094020548 1107 16 -0.8404485508263022 1107 17 -0.45552077798182466 1107 18 -0.8482281705859194 1107 19 0.1845077348729518 1107 20 0.28187018248681994 1107 21 0.08173075153806053 1107 22 -0.07093944303758093 1107 23 -0.3146627531711417 1107 24 -4.606392529736681 1107 25 -2.325353567801045 1108 1 0.3866858107732014 1108 2 -0.17135132741788794 1108 3 0.47669590614821544 1108 4 0.42332432341492776 1108 5 0.7280459088361972 1108 6 -2.9034137726016027 1108 7 -1.0556030436873094 1108 8 0.22890914270010398 1108 9 0.1372600707103615 1108 10 0.47608472200140994 1108 11 0.2771317232229791 1108 12 0.44425279246659954 1108 13 0.29036937368879023 1108 14 0.26945233507603206 1108 15 -0.8755361792515401 1108 16 0.25494721954385113 1108 17 0.18367025107350798 1108 18 0.1822288673266499 1108 19 0.2797230315604651 1108 20 -1.3959295908457108 1108 21 -0.25796607137704797 1108 22 -0.011343386153775725 1108 23 -0.04043158976110561 1108 24 -0.8749930077581638 1108 25 -0.2951957608648712 1109 1 0.8416692565503148 1109 2 0.7253726451546498 1109 3 -0.5298173813256923 1109 4 0.8569971928625542 1109 5 1.1912844182579576 1109 6 1.2094378898024871 1109 7 1.2700911864035298 1109 8 1.8182050278457638 1109 9 1.1119524120396918 1109 10 1.1919891778617213 1109 11 0.857165695207577 1109 12 0.5719090929928043 1109 13 0.6506827831605264 1109 14 0.7207115400323758 1109 15 -0.5878597049886736 1109 16 0.7051899290714374 1109 17 0.2559172158264218 1109 18 0.6329878628288981 1109 19 -0.5854620229710932 1109 20 0.4453607015081076 1109 21 0.7097554906360628 1109 22 0.9294826162861796 1109 23 0.9229222112795554 1109 24 -0.46187374018606775 1109 25 0.32882954265983244 1110 1 0.3351908853979175 1110 2 0.3917866022504768 1110 3 0.5984959032669543 1110 4 0.38790639619563194 1110 5 0.41419576693398596 1110 6 0.3490316994933629 1110 7 0.5402471270275342 1110 8 0.9190576538073714 1110 9 0.4786487855229353 1110 10 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21 0.4505756913954144 2210 22 0.5982947034552666 2210 23 0.6326273134795883 2210 24 0.9361212668353112 2210 25 0.7582907170500164 2211 1 0.9080963968464815 2211 2 0.5636006285340187 2211 3 0.5795614540426826 2211 4 0.8556139125653068 2211 5 1.0473687691131346 2211 6 -1.9080935264337313 2211 7 0.37099266689449095 2211 8 0.8140715807307579 2211 9 1.0358538296139983 2211 10 0.9972816206851007 2211 11 0.8726188236275618 2211 12 0.9194819721919387 2211 13 0.7621715419035056 2211 14 0.8781649041682869 2211 15 1.4365373363333944 2211 16 0.3081286686292657 2211 17 0.42220948407823755 2211 18 0.5561586496504276 2211 19 0.8179303368200195 2211 20 0.6561665043733882 2211 21 0.04525900555041318 2211 22 -2.1863097303899117 2211 23 -0.8605762561238564 2211 24 1.3777858186706915 2211 25 0.8198018885738421 2212 1 -0.2900776921402901 2212 2 0.05097750494428155 2212 3 -2.666681503085311 2212 4 -1.202488092542044 2212 5 -0.08699639282225391 2212 6 0.6739618437139505 2212 7 0.5965638657703352 2212 8 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0.7871721042269257 2213 20 -0.9460065519281073 2213 21 0.12104393788246881 2213 22 0.03921037085996204 2213 23 0.10204660891768924 2213 24 -0.12135182433684122 2213 25 0.10724505424887099 2214 1 1.2355949528781807 2214 2 1.05311079194378 2214 3 0.8783982618991786 2214 4 1.1338899207660624 2214 5 1.4052233840031079 2214 6 1.1709565796754404 2214 7 1.5972620909270319 2214 8 -1.9200189973965145 2214 9 -0.3896619493189526 2214 10 -0.08054540745632721 2214 11 0.3939617386927736 2214 12 -0.4952445410295745 2214 13 0.0811183695092339 2214 14 0.3407567168271086 2214 15 -1.323319690351795 2214 16 0.24970072060230564 2214 17 0.4314786970377895 2214 18 0.4803769023915123 2214 19 1.339458145277014 2214 20 -0.691730865068152 2214 21 0.1811122885534786 2214 22 0.5444454036741604 2214 23 0.22067375410254897 2214 24 -1.016294811210372 2214 25 -0.01694133704681633 2215 1 1.2959271794497553 2215 2 0.620206604910944 2215 3 0.8936107136925848 2215 4 1.1724887131541628 2215 5 1.7031761413480702 2215 6 1.9959648251647941 2215 7 1.2934072389611764 2215 8 0.07079518469283386 2215 9 0.7413030814273895 2215 10 0.5905612552799226 2215 11 0.5753388915652877 2215 12 0.2887758148556472 2215 13 0.678139786453734 2215 14 0.696355918252415 2215 15 0.8807677872444657 2215 16 1.0220349519894043 2215 17 0.3246529552674744 2215 18 -0.48844867332645237 2215 19 0.830350402438782 2215 20 -2.8015939175825864 2215 21 -1.3701902702522872 2215 22 -0.6168138977329463 2215 23 -0.3050837835696285 2215 24 -0.15874030528847172 2215 25 -0.011845129514895802 2216 1 0.5982815223847133 2216 2 -0.416900766518264 2216 3 0.5227275925363237 2216 4 0.16740789131042533 2216 5 0.5333292792895605 2216 6 -0.8805166338045645 2216 7 -8.694239104545943 2216 8 -9.866209373819451 2216 9 -5.023840156792168 2216 10 -2.7571109199246386 2216 11 -1.7537958961304483 2216 12 -1.3837406933093703 2216 13 -1.1104099761868689 2216 14 -1.0523514422857545 2216 15 -2.418559691262036 2216 16 -1.3318205707439112 2216 17 -1.6016814555370473 2216 18 -1.3234697977185803 2216 19 -0.8003195995438475 2216 20 -1.6909881573748287 2216 21 -3.98114442857651 2216 22 -2.771124543069032 2216 23 -2.639674443585605 2216 24 -2.817131446120694 2216 25 -2.300306881844296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72662.0 3 16.0 4 544.0 5 2817.0 6 3329.0 7 2770.0 8 2238.0 9 2075.0 10 1998.0 11 2015.0 12 2001.0 13 1945.0 14 1897.0 15 2076.0 16 2186.0 17 2371.0 18 2729.0 19 3315.0 20 4353.0 21 5758.0 22 7033.0 23 8285.0 24 9745.0 25 10996.0 26 12535.0 27 13618.0 28 15250.0 29 17981.0 30 21250.0 31 25943.0 32 32900.0 33 43219.0 34 57590.0 35 79650.0 36 114260.0 37 176448.0 38 189481.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.335725572406155 18.24166231237186 12.211436161841268 26.211175953380717 2 14.253502996428605 19.76730900060694 41.87809037819888 24.101097624765586 3 19.89076995649667 23.710362178311232 28.847843178363647 27.551024686828452 4 11.615694999622898 16.229123195146276 38.436051822242334 33.71912998298849 5 12.568019357966367 37.59411722207738 36.040286516330404 13.797576903625849 6 31.612149361466436 37.065729641212926 18.958012897362416 12.364108099958223 7 27.759428366253786 30.620746710229312 23.356374501573757 18.26345042194314 8 25.271016224229047 37.60369795680118 20.711770597338024 16.413515221631744 9 25.995234016890343 15.439398095700774 20.156067100686627 38.409300786722255 10 13.919556996953725 29.727929695885695 35.31342905343494 21.039084253725637 11 34.592777946457545 22.42511490582434 24.496560677604094 18.485546470114016 12 23.176451865532563 24.680876458985086 32.25126581483296 19.891405860649385 13 28.999774900930237 22.249524936265555 25.61533186408204 23.13536829872217 14 20.027598568559828 22.633129589727805 27.70431421326281 29.63495762844956 15 22.74507586822078 31.96578941582569 22.91259005196081 22.37654466399272 16 21.4785738815784 28.47085528575138 28.64968547664318 21.40088535602704 17 21.276658119595478 29.190359573784193 28.191305021552648 21.341677285067682 18 20.996035165267752 28.187388956883076 30.16949223522662 20.647083642622547 19 23.750624308287357 27.06885557615933 27.101000254435075 22.07951986111824 20 23.02599893625486 28.014084153129044 28.311116136678688 20.648800773937406 21 22.377591627641095 27.210585221270534 27.19498546290974 23.21683768817863 22 22.373034677209112 28.929677120119607 27.517293584177118 21.179994618494163 23 21.65372756160253 28.52201043853358 27.83434455536768 21.989917444496214 24 23.195045726520334 27.309228538374157 28.47063504111984 21.025090693985668 25 22.13765073674909 27.97981870758484 28.190782103603446 21.691748452062626 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 264.0 1 264.0 2 333.0 3 402.0 4 402.0 5 402.0 6 602.0 7 802.0 8 802.0 9 802.0 10 1262.5 11 1723.0 12 1723.0 13 1723.0 14 2895.0 15 4067.0 16 4067.0 17 4067.0 18 7293.5 19 10520.0 20 10520.0 21 10520.0 22 16714.5 23 22909.0 24 22909.0 25 22909.0 26 33318.0 27 43727.0 28 43727.0 29 43727.0 30 58244.0 31 72761.0 32 72761.0 33 72761.0 34 84171.5 35 95582.0 36 95582.0 37 95582.0 38 103872.0 39 112162.0 40 112162.0 41 112162.0 42 118036.0 43 123910.0 44 123910.0 45 123910.0 46 126231.5 47 128553.0 48 128553.0 49 128553.0 50 122216.0 51 115879.0 52 115879.0 53 115879.0 54 101926.0 55 87973.0 56 87973.0 57 87973.0 58 74558.0 59 61143.0 60 61143.0 61 61143.0 62 49454.0 63 37765.0 64 37765.0 65 37765.0 66 28895.5 67 20026.0 68 20026.0 69 20026.0 70 14578.5 71 9131.0 72 9131.0 73 9131.0 74 6444.0 75 3757.0 76 3757.0 77 3757.0 78 2612.0 79 1467.0 80 1467.0 81 1467.0 82 983.0 83 499.0 84 499.0 85 499.0 86 339.5 87 180.0 88 180.0 89 180.0 90 112.0 91 44.0 92 44.0 93 44.0 94 33.0 95 22.0 96 22.0 97 22.0 98 16.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22998516663718138 2 0.13713271201397706 3 0.1391216597454775 4 0.06605400097772483 5 0.023029921101583934 6 0.02093629191053085 7 0.29111913901593145 8 0.28818805814845716 9 0.018528618340819803 10 0.03580105916700775 11 0.015597537473345484 12 0.01706307790708264 13 0.015074130175582212 14 0.016434989149766717 15 0.014236678499160977 16 0.019156707098135727 17 0.018423936881267148 18 0.013817952660950362 19 0.023239284020689244 20 0.01706307790708264 21 0.014236678499160977 22 0.016330307690214065 23 0.017167759366635296 24 0.015074130175582212 25 0.014341359958713632 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 955279.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.46868393861492 #Duplication Level Percentage of deduplicated Percentage of total 1 93.92416592554497 81.21499017613972 2 4.614949220399281 7.980971710629254 3 0.7431784595163425 1.9278498997771603 4 0.25161364419358606 0.8702680269767322 5 0.12380522441148004 0.5352637409792779 6 0.07291257066156254 0.37827924166119464 7 0.05059466910185369 0.3062398116082894 8 0.03078286795818578 0.2129403264160386 9 0.027829523869580403 0.2165744073276799 >10 0.13452483290485098 2.1609780275101307 >50 0.013309775162420845 0.8104215152902005 >100 0.010761397990290378 1.9557722767719685 >500 9.673158681392715E-4 0.5746241815873685 >1k 6.045724175870446E-4 0.8548266573250227 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2282 0.23888309069915703 No Hit CTGTAGGACGTGGAATATGGCAAGA 1726 0.18068019918788125 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1550 0.16225626230661408 No Hit GTCCTACAGTGTGCATTTCTCATTT 1414 0.14801958380745311 No Hit CTTTAGGACGTGAAATATGGCGAGG 1204 0.12603647730139572 No Hit CTGTAGGACCTGGAATATGGCGAGA 998 0.10447209663354894 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0468145955265425E-4 0.0 5 0.0 0.0 0.0 1.0468145955265425E-4 0.0 6 0.0 0.0 0.0 1.0468145955265425E-4 0.0 7 0.0 0.0 0.0 2.093629191053085E-4 0.0 8 0.0 0.0 0.0 2.093629191053085E-4 0.0 9 0.0 0.0 0.0 2.093629191053085E-4 0.0 10 0.0 0.0 0.0 2.093629191053085E-4 0.0 11 0.0 0.0 0.0 2.093629191053085E-4 0.0 12 0.0 0.0 0.0 2.093629191053085E-4 0.0 13 0.0 0.0 0.0 2.093629191053085E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCCGTA 40 0.0051635127 14.296815 2 TCGAACT 55 1.9883669E-4 13.793883 19 CGCCTTG 70 7.0137467E-6 13.6160145 2 CCTACAC 195 0.0 13.196365 3 TAGGACC 435 0.0 13.131983 4 TCGCCTT 80 1.9022227E-6 13.119224 1 CTACACT 220 0.0 12.549997 4 GGCGAGG 405 0.0 11.941927 19 TAGGACA 375 0.0 11.67871 4 GTAGGAC 1140 0.0 11.369969 3 AGGACCT 685 0.0 11.255081 5 GTCCTAC 1165 0.0 11.220187 1 TGTAGGA 1215 0.0 11.217803 2 AGGACGT 900 0.0 11.210307 5 TCCAACG 110 5.0740164E-7 11.20753 18 TATACTG 205 0.0 11.143242 5 GACGTGA 500 0.0 11.041096 7 GGACGTG 900 0.0 10.893035 6 CTGTAGG 1220 0.0 10.870772 1 GGACATG 405 0.0 10.810779 6 >>END_MODULE