FastQCFastQC Report
Thu 26 May 2016
SRR1926777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926777_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955279
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT23380.24474525243410564No Hit
CTGTAGGACGTGGAATATGGCAAGA21730.22747281160791769No Hit
GTCCTAAAGTGTGTATTTCTCATTT16320.17084014198993175No Hit
CTTTAGGACGTGAAATATGGCGAGG15730.16466393587632513No Hit
GTCCTACAGTGTGCATTTCTCATTT15720.16455925441677247No Hit
GTATCAACGCAGAGTACTTTTTTTT14460.15136939051313805No Hit
CTGTAGGACCTGGAATATGGCGAGA12700.13294545363187088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4550.014.6506724
GCGTAGA400.005150045314.30244351
CCAACGA551.9808352E-413.80005819
GGTCTAT801.9163726E-613.1105741
TATTAAG603.9681594E-412.7119462
TGTAGGA11800.012.4426252
AGGACCT7350.012.0494315
GTAGGAC11550.011.7915433
GGACCTG7200.011.7714096
TCCAACG907.5616845E-611.59588218
CTAGGAC1406.511982E-1011.5647823
CTAAATT6700.011.47001219
CTGTAGG12400.011.4573341
TCGAACT752.0976311E-411.38504819
GTCTTAG1604.1836756E-1111.3227681
TCTAGGA1354.458343E-911.2995082
GTCCTAC13850.011.2904981
TAAACCT855.188565E-511.2034564
TCAATAC600.005763830611.111263
TAGGACA3700.011.0671984
Produced by FastQC (version 0.11.5)