FastQCFastQC Report
Thu 26 May 2016
SRR1926776_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926776_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613808
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12700.2069050908427391No Hit
GTCCTACAGTGGACATTTCTAAATT12620.20560175168782419No Hit
GTACATGGGAAGCAGTGGTATCAAC9970.1624286421812684No Hit
GTCCTACAGTGTGCATTTCTCATTT9910.16145113781508225No Hit
CTGTAGGACGTGGAATATGGCAAGA9570.15591194640669395No Hit
GTCCTAAAGTGTGTATTTCTCATTT9100.14825482887156896No Hit
CCCATGTACTCTGCGTTGATACCAC7670.12495764147746527No Hit
TATCAACGCAGAGTACTTTTTTTTT6940.11306467168886687No Hit
GGTATCAACGCAGAGTACTTTTTTT6800.1107838281677658No Hit
CTTTAGGACGTGAAATATGGCGAGG6640.10817714985793603No Hit
CTGTAGGACCTGGAATATGGCGAGA6290.10247504105518339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA453.5714278E-516.8677419
GGACGAG350.0021509516.3051786
TCCAACG601.48252E-615.81350618
TCGCCTT400.00516494314.2950531
GTCTAGG1009.591531E-913.342051
TAGGACC2650.013.28760054
GTATAAG655.283116E-513.1954331
GGCGAGG1650.012.65080619
AGGACCT3600.012.1533975
ATAATAC802.7874043E-511.904753
CCTGCAC1209.598807E-911.904753
AGACAGT802.8249755E-511.8891936
GTCCTAA2450.011.6694321
GTCCTAC6450.011.52469351
CTGTAGG6800.011.4921011
GGACCTG3450.011.3033486
TGGCGAG4550.011.26060718
GTCCTGC855.1423376E-511.2118051
TGTAGGA7400.011.1968992
GTAGGAC7000.011.0203973