##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926776_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 613808 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.234969241195945 30.0 26.0 31.0 10.0 31.0 2 26.84627114667779 31.0 27.0 31.0 10.0 34.0 3 26.44919258139353 31.0 26.0 31.0 10.0 34.0 4 30.12888069233376 35.0 30.0 37.0 10.0 37.0 5 29.685499374397207 35.0 30.0 37.0 10.0 37.0 6 29.517119359799807 35.0 29.0 37.0 8.0 37.0 7 29.60202213069885 35.0 30.0 37.0 2.0 37.0 8 29.291982183353753 35.0 29.0 37.0 2.0 37.0 9 31.4090627688137 37.0 31.0 39.0 2.0 39.0 10 31.59585081980033 37.0 32.0 39.0 2.0 39.0 11 31.820443852149207 37.0 32.0 39.0 2.0 39.0 12 31.68656974167818 37.0 32.0 39.0 2.0 39.0 13 31.791350063863618 37.0 32.0 39.0 2.0 39.0 14 32.683314652138776 38.0 32.0 40.0 2.0 41.0 15 32.268891901050495 38.0 32.0 40.0 2.0 41.0 16 32.32512446888929 38.0 32.0 40.0 2.0 41.0 17 32.5399457159242 38.0 32.0 40.0 2.0 41.0 18 32.58156948101035 38.0 32.0 40.0 2.0 41.0 19 31.940147407658422 38.0 31.0 40.0 2.0 41.0 20 32.235037666501576 38.0 32.0 40.0 2.0 41.0 21 32.23906498449026 38.0 32.0 40.0 2.0 41.0 22 32.253484803065454 38.0 32.0 40.0 2.0 41.0 23 32.23548894768397 39.0 32.0 40.0 2.0 41.0 24 30.914528647394626 38.0 29.0 40.0 2.0 41.0 25 31.47280419935876 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.183895922623531 1101 2 -1.2612687483563292 1101 3 -2.1875781945132786 1101 4 -1.6631918824518088 1101 5 -1.975491470259609 1101 6 -4.093927499009155 1101 7 -2.192206927893139 1101 8 -1.6069520506197286 1101 9 -1.625475929053355 1101 10 -10.1534560149682 1101 11 -5.399621127831786 1101 12 -2.936283213310684 1101 13 -2.4225972735527996 1101 14 -2.142455554091569 1101 15 -3.2840335452791756 1101 16 -2.0164654613603545 1101 17 -1.575862363002532 1101 18 -1.693616113880637 1101 19 -2.495579384391938 1101 20 -1.5857727994867972 1101 21 -1.722724399607106 1101 22 -1.280413775156994 1101 23 -1.604773631990689 1101 24 -1.947985648184826 1101 25 -1.5362222551577709 1102 1 -1.1854867245338205 1102 2 -1.0600561078465631 1102 3 -1.3289115898402848 1102 4 -1.0207160323082292 1102 5 -1.157804896981979 1102 6 -1.9897161360118503 1102 7 -1.039016135724708 1102 8 -0.508348900898369 1102 9 -0.6828532867501984 1102 10 -0.4757898996024039 1102 11 -0.6062050772272372 1102 12 -0.51521665646138 1102 13 -0.7328136093282431 1102 14 -1.2794217166168345 1102 15 -0.76747569964785 1102 16 -0.9515691568474374 1102 17 -0.8771195061127095 1102 18 -1.1471717830912596 1102 19 -0.7691497106779188 1102 20 -0.9011390381757174 1102 21 -1.045002932748261 1102 22 -1.0053777413350176 1102 23 -1.4566818193621351 1102 24 -3.9549145480849717 1102 25 -2.709743650723894 1103 1 -0.5009437396713459 1103 2 -0.4972354843230633 1103 3 -0.11169461862970209 1103 4 -0.3295120487719778 1103 5 -0.7686307633988996 1103 6 0.2771743720927162 1103 7 0.10820887252266331 1103 8 0.12797518430750543 1103 9 -0.15770046127788717 1103 10 -0.03807139958358263 1103 11 -0.12623235444301173 1103 12 -0.2075929846204545 1103 13 -0.03382388477941234 1103 14 9.95589359575888E-4 1103 15 0.2897710285253936 1103 16 -0.001912446807338597 1103 17 -0.2234715106116809 1103 18 -0.3796867999513225 1103 19 -1.9488018376143934 1103 20 -0.6159183296323256 1103 21 -0.28509446197717025 1103 22 -0.38644288118609893 1103 23 -0.3251478523649105 1103 24 1.293178592979416 1103 25 0.6456904267549106 1104 1 -1.218231840137019 1104 2 -0.919218934343899 1104 3 -2.1307488993662282 1104 4 -1.2888311809883035 1104 5 -1.5183063915792019 1104 6 -2.7008557788346295 1104 7 -0.9246935482302234 1104 8 -0.6138863753110613 1104 9 -0.6491394200065628 1104 10 -0.6274425824127086 1104 11 -0.608796154763823 1104 12 -0.6515020266603386 1104 13 -0.8394425875943767 1104 14 -0.8865699056825562 1104 15 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0.0568182121487979 1108 24 -0.9978431208106038 1108 25 -0.28853219954394405 1109 1 0.56286450787184 1109 2 0.37165796890773706 1109 3 -0.9609669679020527 1109 4 0.26383430963462473 1109 5 0.8067915078065013 1109 6 0.7785165390975344 1109 7 0.8328162417083469 1109 8 1.3166988941155537 1109 9 0.7080682267799574 1109 10 0.9135437929954655 1109 11 0.354586972761858 1109 12 0.2055898924178443 1109 13 0.41741349718085985 1109 14 0.6338575987276087 1109 15 -0.9427390533822759 1109 16 0.1695446915172667 1109 17 0.22014495107707432 1109 18 0.5320787654287003 1109 19 -0.9417987733824127 1109 20 0.01062944149375511 1109 21 0.12383856743778665 1109 22 0.4706862590755705 1109 23 0.3291804796742639 1109 24 -0.36141274534686474 1109 25 -0.08254475931159888 1110 1 0.7902496582293672 1110 2 1.0666452350454598 1110 3 1.2087484792524172 1110 4 1.1520579358467877 1110 5 1.0112189737679103 1110 6 1.4778382160492427 1110 7 1.4597630533451351 1110 8 1.5526916456093325 1110 9 1.413818055362583 1110 10 1.3202437636096285 1110 11 1.1516165545034553 1110 12 1.1901133521102736 1110 13 1.1421539222959538 1110 14 1.0985945954951646 1110 15 1.4720175339426333 1110 16 1.2837453967773342 1110 17 0.7278832212308615 1110 18 1.1141142243375057 1110 19 -0.9169554978412222 1110 20 0.7900152775207943 1110 21 0.7052373332326738 1110 22 0.9357538464984358 1110 23 0.8988371551566843 1110 24 0.032137063645201636 1110 25 0.5418797680662024 1111 1 0.7975216995126537 1111 2 0.37721312365847837 1111 3 1.1478154672276872 1111 4 0.933987978201106 1111 5 1.3566135504681682 1111 6 1.1407258554046784 1111 7 1.2505229968727143 1111 8 1.4278681219249947 1111 9 0.900953540489887 1111 10 0.9683378194004852 1111 11 0.5081413098707728 1111 12 0.4387380539261514 1111 13 0.49668266967923813 1111 14 0.8293160793567154 1111 15 0.11608306142425562 1111 16 0.05458785819595846 1111 17 0.4668443234167299 1111 18 0.6300214343078068 1111 19 1.3723500374296975 1111 20 0.6848232226062336 1111 21 0.9331717083848119 1111 22 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2213 22 -0.18058076664588185 2213 23 -0.1342646226320312 2213 24 -0.7694799283067546 2213 25 -0.4681679787281148 2214 1 1.6862291939804592 2214 2 1.2011126083912274 2214 3 1.2623730723393969 2214 4 1.5282561822356904 2214 5 1.7917272265365547 2214 6 1.5800142805664272 2214 7 2.050944287089063 2214 8 -1.0895357022174643 2214 9 0.0956366089605929 2214 10 0.5896324534723796 2214 11 0.8107031881263325 2214 12 -0.21010912093940703 2214 13 0.39983210521460677 2214 14 0.8106351313934823 2214 15 -0.3847040966539552 2214 16 0.8034101331631263 2214 17 1.1292017012164663 2214 18 1.0283435024451748 2214 19 2.2282960930046656 2214 20 0.2847601696377211 2214 21 1.1003805502584072 2214 22 1.6592554250755853 2214 23 1.034502774891351 2214 24 -0.2657968814890168 2214 25 0.8055226006715941 2215 1 1.6148006225518898 2215 2 1.0431649222745243 2215 3 1.2181074787780268 2215 4 1.6097451158373026 2215 5 2.1629545907216645 2215 6 2.4693502966630057 2215 7 1.692795393728904 2215 8 0.622737939633641 2215 9 1.0634434500068721 2215 10 0.9497934192671487 2215 11 0.7986307535186867 2215 12 0.4639351446541511 2215 13 0.8616027289570596 2215 14 1.2221039442707493 2215 15 0.9653965069677781 2215 16 1.2903316623381755 2215 17 0.7630045181178744 2215 18 0.14504370365241925 2215 19 1.2695435779141206 2215 20 -2.6136301724145916 2215 21 -0.8131003350534662 2215 22 0.12002001260074024 2215 23 0.31216877086720274 2215 24 0.605430482696093 2215 25 0.715985377331549 2216 1 0.37900260866209834 2216 2 -0.4208462414822094 2216 3 0.48612980629149405 2216 4 0.20745394948434281 2216 5 0.5853522021969688 2216 6 -1.3787641967490778 2216 7 -8.920048118960135 2216 8 -9.711803502567953 2216 9 -4.723095136349979 2216 10 -2.806391431774582 2216 11 -1.6467849666085286 2216 12 -1.0726456303182594 2216 13 -0.8136669536547387 2216 14 -1.125866458792796 2216 15 -2.093854482842051 2216 16 -0.9331927097005064 2216 17 -1.435253416587134 2216 18 -1.3018181124052184 2216 19 -0.6112054322760514 2216 20 -2.024600510572572 2216 21 -4.743978507312828 2216 22 -2.924318275190526 2216 23 -2.79277443676569 2216 24 -2.690239018180133 2216 25 -2.0687968645065737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55082.0 3 10.0 4 396.0 5 2134.0 6 2660.0 7 2240.0 8 1830.0 9 1626.0 10 1441.0 11 1460.0 12 1401.0 13 1388.0 14 1406.0 15 1486.0 16 1526.0 17 1699.0 18 2043.0 19 2428.0 20 3075.0 21 3948.0 22 4720.0 23 5717.0 24 6540.0 25 7224.0 26 8371.0 27 8857.0 28 10245.0 29 11835.0 30 14217.0 31 17037.0 32 21726.0 33 28508.0 34 37678.0 35 52121.0 36 73639.0 37 109640.0 38 106454.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5667340335572 18.485844790602155 12.586235605632698 25.361185570207944 2 13.712856832939957 20.196487496472653 43.038362854304665 23.052292816282723 3 20.022151139935502 24.954123651643147 28.56355240343131 26.46017280499004 4 11.074844749357233 16.777340661239755 39.03349240172622 33.11432218767679 5 11.829111696302864 38.69544245711008 35.87908040882307 13.596365437763987 6 30.34630087699573 37.55031074523453 19.904804737276066 12.198583640493672 7 27.34795173641158 31.410354792887812 23.25403693242118 17.987656538279424 8 25.300783858490767 37.4896935914956 20.94115265240015 16.268369897613486 9 26.438789427286935 15.81784377530785 20.50292247100749 37.24044432639773 10 13.642586592433714 30.577739241546116 35.34594598118534 20.433728184834834 11 33.75975415218514 23.50593844353957 24.30321665012799 18.4310907541473 12 22.751070143049652 26.059184980356946 32.314002049851474 18.875742826741927 13 28.41993948350787 23.09500958911029 25.76171101664122 22.723339910740616 14 19.763142757979022 23.679858172438458 28.543285595801226 28.013713473781294 15 22.734108821435147 32.269386252059164 23.460660462979106 21.535844463526583 16 20.486502076791588 29.644590809321365 29.441555711624982 20.427351402262065 17 20.52136902550465 29.844938503353475 28.53125020368524 21.102442267456638 18 19.945675729401913 29.004989229651212 30.952613724129165 20.09672131681771 19 22.916123481253585 27.778393387912605 28.226358398081036 21.079124732752778 20 22.425196388504613 28.600618862095427 29.258424622824897 19.71576012657506 21 22.140607407576383 27.675026029658333 27.986078409966712 22.198288152798575 22 21.471193046833502 29.61874252492236 28.43641743766559 20.47364699057855 23 20.943063571570566 29.035101726896766 28.647291356391214 21.374543345141454 24 22.26494904825995 28.112097086935695 29.273868454241196 20.34908541056316 25 21.388802440188815 28.447198491827745 29.009504287765004 21.15449478021844 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 354.0 1 354.0 2 401.5 3 449.0 4 449.0 5 449.0 6 655.0 7 861.0 8 861.0 9 861.0 10 1209.5 11 1558.0 12 1558.0 13 1558.0 14 2434.5 15 3311.0 16 3311.0 17 3311.0 18 5525.0 19 7739.0 20 7739.0 21 7739.0 22 12199.5 23 16660.0 24 16660.0 25 16660.0 26 23776.5 27 30893.0 28 30893.0 29 30893.0 30 40768.5 31 50644.0 32 50644.0 33 50644.0 34 58157.5 35 65671.0 36 65671.0 37 65671.0 38 70929.5 39 76188.0 40 76188.0 41 76188.0 42 79091.0 43 81994.0 44 81994.0 45 81994.0 46 83556.0 47 85118.0 48 85118.0 49 85118.0 50 79866.5 51 74615.0 52 74615.0 53 74615.0 54 63066.0 55 51517.0 56 51517.0 57 51517.0 58 42138.0 59 32759.0 60 32759.0 61 32759.0 62 25435.0 63 18111.0 64 18111.0 65 18111.0 66 13607.0 67 9103.0 68 9103.0 69 9103.0 70 6452.0 71 3801.0 72 3801.0 73 3801.0 74 2670.0 75 1539.0 76 1539.0 77 1539.0 78 1063.5 79 588.0 80 588.0 81 588.0 82 402.5 83 217.0 84 217.0 85 217.0 86 149.0 87 81.0 88 81.0 89 81.0 90 52.5 91 24.0 92 24.0 93 24.0 94 17.5 95 11.0 96 11.0 97 11.0 98 6.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20234340380053698 2 0.12072178922399186 3 0.12169929359017805 4 0.07249824049214086 5 0.023460104788468054 6 0.019875922112452104 7 0.21798347365951568 8 0.21586554753277898 9 0.018735500351901573 10 0.03975184422490421 11 0.015477152464614343 12 0.017106326408257957 13 0.014825482887156896 14 0.017106326408257957 15 0.01417381330969945 16 0.01971300471808774 17 0.018898417746265935 18 0.014010895915335088 19 0.023460104788468054 20 0.01645465683080051 21 0.013847978520970725 22 0.016617574225164872 23 0.01726924380262232 24 0.014662565492792535 25 0.013847978520970725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 613808.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.51887492486264 #Duplication Level Percentage of deduplicated Percentage of total 1 95.201915406083 83.31964527032333 2 3.6583554655027726 6.403503088320496 3 0.5299083966731962 1.391309600702279 4 0.18946275956987696 0.6632627023086153 5 0.09598601193481952 0.42002938865299216 6 0.06335980864547734 0.3327107500864057 7 0.04136059564568277 0.253388295799263 8 0.030236534847736488 0.21170140092002404 9 0.026836107350782363 0.21137993304329777 >10 0.13299894453293573 2.243463778799828 >50 0.015255154441400843 0.9041309155549243 >100 0.01209237610699547 1.9648767664163014 >500 0.0018603655544937878 1.2683452111676807 >1k 3.720731108987575E-4 0.4122528979045658 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1270 0.2069050908427391 No Hit GTCCTACAGTGGACATTTCTAAATT 1262 0.20560175168782419 No Hit GTACATGGGAAGCAGTGGTATCAAC 997 0.1624286421812684 No Hit GTCCTACAGTGTGCATTTCTCATTT 991 0.16145113781508225 No Hit CTGTAGGACGTGGAATATGGCAAGA 957 0.15591194640669395 No Hit GTCCTAAAGTGTGTATTTCTCATTT 910 0.14825482887156896 No Hit CCCATGTACTCTGCGTTGATACCAC 767 0.12495764147746527 No Hit TATCAACGCAGAGTACTTTTTTTTT 694 0.11306467168886687 No Hit GGTATCAACGCAGAGTACTTTTTTT 680 0.1107838281677658 No Hit CTTTAGGACGTGAAATATGGCGAGG 664 0.10817714985793603 No Hit CTGTAGGACCTGGAATATGGCGAGA 629 0.10247504105518339 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 1.629173943643615E-4 0.0 13 0.0 0.0 0.0 1.629173943643615E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 45 3.5714278E-5 16.86774 19 GGACGAG 35 0.00215095 16.305178 6 TCCAACG 60 1.48252E-6 15.813506 18 TCGCCTT 40 0.005164943 14.295053 1 GTCTAGG 100 9.591531E-9 13.34205 1 TAGGACC 265 0.0 13.2876005 4 GTATAAG 65 5.283116E-5 13.195433 1 GGCGAGG 165 0.0 12.650806 19 AGGACCT 360 0.0 12.153397 5 ATAATAC 80 2.7874043E-5 11.90475 3 CCTGCAC 120 9.598807E-9 11.90475 3 AGACAGT 80 2.8249755E-5 11.889193 6 GTCCTAA 245 0.0 11.669432 1 GTCCTAC 645 0.0 11.5246935 1 CTGTAGG 680 0.0 11.492101 1 GGACCTG 345 0.0 11.303348 6 TGGCGAG 455 0.0 11.260607 18 GTCCTGC 85 5.1423376E-5 11.211805 1 TGTAGGA 740 0.0 11.196899 2 GTAGGAC 700 0.0 11.020397 3 >>END_MODULE