FastQCFastQC Report
Thu 26 May 2016
SRR1926776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926776_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613808
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23100.37633918098167507No Hit
GGTATCAACGCAGAGTACTTTTTTT14780.2407919088705263No Hit
TATCAACGCAGAGTACTTTTTTTTT13250.21586554753277898No Hit
GTCCTACAGTGGACATTTCTAAATT12760.20788259520892527No Hit
GTACATGGGAAGCAGTGGTATCAAC11860.19322002971613272No Hit
CTGTAGGACGTGGAATATGGCAAGA11160.18181581211062744No Hit
GTCCTACAGTGTGCATTTCTCATTT10700.17432161196986679No Hit
CCCATGTACTCTGCGTTGATACCAC9690.1578669551390663No Hit
GTCCTAAAGTGTGTATTTCTCATTT8960.1459739853504679No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8910.14515939837864608No Hit
CTTTAGGACGTGAAATATGGCGAGG7900.12870474154784559No Hit
CTGTAGGACCTGGAATATGGCGAGA7800.12707556760420197No Hit
CACACACACACACACACACACACAC6670.1086659020410291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG250.00606216718.9814218
AAGGCGT400.005194850314.2826226
CTAGGAC1006.348273E-1014.2826223
CCCGTTT400.00532012214.23142612
ATAACAC707.076207E-613.6024973
CCTATAC655.326436E-513.1839593
TAGGACC3000.013.0130564
GTATTAG751.427564E-512.7070921
TATTAGG603.9800958E-412.7060532
AGGACGT5400.012.5193365
GACGTGA2750.012.4201537
TATACTG1155.118636E-912.4196725
GGACGTG5300.012.2165826
CTCTAGG1251.3333192E-912.198811
TTAGGAC3450.012.1436793
TCTTACA550.002994340112.1285042
CTAGACC550.003012746812.1185883
TAGGACG5600.012.0722164
TCTAGGA1353.5106495E-1012.0001612
CTGTAGG6000.011.9128991
Produced by FastQC (version 0.11.5)